Rational design yields molecular insights on leaf binding of the anchor peptide Macaque Histatin

In times of a constantly growing world population and increasing demand for food, sustainable agriculture is crucial. To reduce the amount of applied nutrients, herbicides, and fungicides, the rainfastness of plant protection agents is of pivotal importance. As a result of protective agent wash-off, plant protection is lost, and soils and groundwater are severely polluted. To date, rainfastness of plant protection products is achieved by adding polymeric adjuvants to the agrochemicals. However, polymeric adjuvants will be regarded as microplastics in the future, and environmentally friendly alternatives are needed. Anchor peptides (APs) are promising biobased and biodegradable adhesion promoters. While the adhesion of anchor peptides to artificial surfaces, such as polymers, has already been investigated in theory and experimentally, exploiting the adhesion to biological surfaces remains challenging. The complex nature and composition of biological surfaces such as plant leaf and fruit surfaces complicate the generation of accurate models. Here, we present the first detailed three-layered atomistic model of the surface of apple leaves and use it to compute free energy profiles of the adhesion and desorption of APs to and from that surface. Our model is validated by a novel fluorescence-based MTP assay that mimicks these complex processes and allows quantifying them. For the AP Macaque Histatin, we demonstrate that aromatic and positively charged amino acids are essential for binding to the waxy apple leaf surface. The established protocols should generally be applicable for tailoring the binding properties of APs to biological interfaces.

Linking the effects of plant protection products on biodiversity and ecological processes to potential impairment of ecosystem functions and services—A multidisciplinary conceptual framework.

There is growing interest in using the ecosystem services framework for environmental risk assessments of plant protection products (PPP). However, there is still a broad gap between most of the ecotoxicological endpoints used in PPP risk assessment and the evaluation of the risks and effects of PPP on ecosystem services. Here we propose a conceptual framework to link current and future knowledge on the ecotoxicological effects of PPP on biodiversity and ecological processes to their consequences on ecosystem functions and services. We first describe the main processes governing the relationships between biodiversity, ecological processes and ecosystem functions in response to effects of PPP. We define 12 main categories of ecosystem functions that could be directly linked with the ecological processes used as functional endpoints in investigations on the ecotoxicology of PPP. An exploration of perceptions on the possible links between these categories of ecosystem functions and groups of ecosystem services (by a panel scientific experts in various fields of environmental sciences) then finds that these direct and indirect linkages still need clarification. We illustrate how the proposed framework could be used on terrestrial microalgae and cyanobacteria to assess the potential effects of herbicides on ecosystem services. The framework proposed here uses a set of clearly-defined core categories of ecosystem functions and services, which should help identify which of them are effectively or potentially threatened by PPP. We argue that this framework could help harmonize and extend the scientific knowledge that informs decision-making and policy-making.

Formation of photosynthetic parameters of winter wheat crops under foliar fertilization and herbicide application

Purpose. To study agronomic measures for the care of winter wheat: the use of foliar fertilization and protection against weeds with sulfonylureas. Methods. Field and laboratory. Results. It was investigated that the application of Bioforge anti-stress fertilizer did not significantly affect the growth of photosynthetic potential of crops, as the leaf surface area was not significantly different. In addition to the area of the photosynthetic leaf surface, there is a more accurate indicator, that is net productivity of photosynthesis, which can, in our opinion, more accurately show whether the effects of plant protection products against weeds lead to changes in physiological processes in the plant. the mechanism of action of the anti-stress agent is somewhat different. After all, the organization of the photosynthetic apparatus of plants can be such that for a relatively small area of leaves you can get better rates of dry matter accumulation. The best indicators of photosynthetic potential for the use of plant protection products in autumn were identified with the use of such products as Granstar Pro 75, WG, Logran 75, WG, Harmony 75, WG, for introduction into the phenophase BBCH 10–13, and in spring for use in BBCH 27–29 in combination with Bioforge and separately. However, the introduction of PIC 75, WG, to form the best photosynthetic potential of winter wheat crops should be carried out in the phase of BBCH 7–9 in autumn or spring in BBCH 25–26. We also investigated that the use of Bioforge anti-stress fertilizer did not significantly affect the growth of photosynthetic potential of crops. Conclusions. It was determined that the use of the anti-stress agent Bioforge had a positive effect on the condition of plants and their accumulation of dry matter. Accordingly, the best indicators of net productivity of wheat photosynthesis were obtained with the use in autumn of such products as Granstar Pro 75, WG, Logran 75, WG, Harmony 75, WG, for introduction into the phenophase BBCH 10–13, and spring for use in BBCH 27–29 in combination with Bioforge. However, the introduction of PIC 75, WG, to form the best photosynthetic potential of winter wheat crops should be carried out in the phase of BBCH 7–9 in autumn or spring in BBCH 25–26 in combination with Bioforge.

Prevalence of pesticides in Krško-Brežice polje aquifer

Groundwater pollution with pesticides is a problem that occurs all over the world as well as in Slovenia. Considering the past high loads of groundwater with pesticides, the purpose of the presented research was to determine the presence of pesticides in the groundwater of Krško-Brežiško polje in the period 2018-2019 and to check the applicability of the passive sampling method. A total of 21 groundwater samples were taken at 11 locations and 2 samples each in the Sava and Krka rivers. We identified 15 pesticides and their degradation products. Atrazine and its degradation product desethylatrazine were most frequently determined in groundwater samples. They are followed by desethylterbutylazine, terbutylazine, metolachlor and simazine. Atrazine, desethylatrazine, chlortoluron, metolachlor and terbuthylazine were detected in surface water. A total of 24 samples were taken in groundwater and surface water using the qualitative passive sampling method. We singled out 8 pesticides that appear in two campaigns. The frequency and occurrence of individual pesticides by both methods are comparable. Passive sampling has proven to be an appropriate method of identifying the presence of pesticides. The highest loads in the Krško-Brežiško field arise from the agricultural land areas. Groundwater is more contaminated with pesticides in the central part of the field in the direction of groundwater flow from west to east. In the groundwater of the Krško-Brežice field, atrazine and desethylatrazine are still the most frequently detected pesticides with higher concentrations, despite a 20 years long ban on the use of atrazine-based plant protection products.

Considering variance in pollinator responses to stressors can reveal potential for resilience

Environmental stressors have sublethal consequences on animals, often affecting the mean of phenotypic traits in a population. However, potential effects on variance are poorly understood. Since phenotypic variance is the basis for adaptation, any influence of stressors may have important implications for population resilience. Here we explored this possibility in insect pollinators by analysing raw datasets from 24 studies (6,913 bees) in which individuals were first exposed to stressors and then tested for cognitive tasks. While all types of stressors decreased the mean cognitive performance of bees, their effect on variance was complex. Focusing on 15 pesticide studies, we found that the dose and the mode of exposure to stressors were critical. At low pesticide doses, cognitive variance decreased following chronic exposures but not for acute exposures. Acute exposure to low doses thus seems less damaging at the population level. In all cases however, the variance decreased with increasing doses. Policy implications: Current guidelines for the authorization of plant protection products on the European market prioritize acute over chronic toxicity assessments on non-target organisms. By overlooking the consequences of a chronic exposure, regulatory authorities may register new products that are harmful to bee populations. Our findings thus call for more research on stress-induced phenotypic variance and its incorporation to policy guidelines to help identify levels and modes of exposure animals can cope with.

RISK ASSESSMENT AND PREDICTION OF THE POSSIBILITY OF ACUTE TOXIC EFFECTS ON WORKERS WHEN APPLYING OBERON RAPID 240 SC

the issue of uncontrolled pesticides application is quite acute in the system of state control over the use of chemical plant protection products and is of a global nature. Indeed, the dangerous effect of chemicals is a potential toxic effect on professional and non-professional contingents. Therefore, the study and forecasting of possible risks of negative effects of pesticides is an integral part of their implementation in the practical activities of agricultural and private farms. The aim was to predict the occurrence of acute toxic effects on workers involved in the treatment of crops with formulation based on the new insecticide spiromesifen and a representative of avermectins, abamectin. Materials and methods. To calculate the coefficients of possible inhalation poisoning, the physic-chemical properties and consumption rates of the studied active ingredients of the Oberon Rapid 240 SC formulation, the methodical approaches from State Standard 8.8.1.002-98 wase used, and to determine the possibility of acute toxic effects, taking into account the selectivity of their action, we used the Sergeev’s S.G. method, taking into account two ways of potential entry of chemicals into the body – inhalation and dermal. Results. According to the "Hygienic classification of pesticides by the degree of hazard", spiromesifen and abamectin, as well as formulation based on them, belong to the Ⅳth hazard class according to the coefficient of possible inhalation poisoning, which was less than 0.5. In terms of inhalation and dermal coefficients of selectivity of action, the studied compounds were classified as substances with a high selectivity of action, except for the value of the coefficient of inhalation action of abamectin, which was less than 100 and made it possible to classify the compound as substances with a relatively low selectivity of action. Conclusions. The obtained data indicate a low possibility of acute poisoning when using formulations based on insecticides – spiromesifene and abamectin, as well as their relative safety in the occurrence of acute toxic effects when inhaled and in contact with the skin.

Electrochemically activated water (ECHAW): history of discovery, specificity of the process, current state and prospects of its application under irrigation conditions

The history of the discovery, the specificity of the process and the current state of the use of electrochemically activated water (ECHAW) in various sectors of the economy are analyzed and the possibilities of its use in irrigation are determined. It has been established that the most promising area of ​​application of ECHAW in irrigated agriculture are drip irrigation systems. The adding an anolyte to water during drip irrigation can significantly reduce (or even eliminate) the need to use plant protection products and the adding an catholyte significantly accelerates the development and increases the yield of agricultural crops against the background of improving product quality. The ecological consequences of this are the reduction of anthropogenic load on irrigated lands through the complete or partial replacement of chemical plant protection products with anolyte, which is an environmentally safe liquid; economic - increasing profitability and reducing the payback period of land irrigation projects. It has been determined that the prospect of using ECHAW in drip irrigation systems is due to the fact that the water supply technology allows supplying catholyte and anolyte to the field with minimal losses of activation potential. A predisposing factor for the use of ECHAW in drip irrigation systems is also their design according to a modular principle, which contributes to the possibility of equipping water treatment units of systems with ECHAW modules. At the same time, the design of systems should be carried out taking into account not only the need to ensure uniform distribution of water by droppers over the field, but also with the preservation of the activation potential of electrochemically activated components of irrigation water. Based on the foregoing, it can be considered that the development of means of electrochemical activation of water for irrigation needs, as well as technologies for the use of such water in growing crops is relevant and timely. The deployment of fundamental and applied research will contribute to the development of domestic equipment for the industrial production of ECHAW and will allow adapting the world technologies of their use to the conditions and needs of the Ukrainian manufacturer and consumer. The use of electrochemically activated water in drip irrigation systems can be especially effective.

Efficiency and Persistence of Movento® Treatment against Myzus persicae and the Transmission of Aphid-Borne Viruses

Neonicotinoids are widely used to protect fields against aphid-borne viral diseases. The recent ban of these chemical compounds in the European Union has strongly impacted rapeseed and sugar beet growing practices. The poor sustainability of other insecticide families and the low efficiency of prophylactic methods to control aphid populations and pathogen introduction strengthen the need to characterize the efficiency of new plant protection products targeting aphids. In this study, the impact of Movento® (Bayer S.A.S., Leverkusen, Germany), a tetrameric acid derivative of spirotetramat, on Myzus persicae and on viral transmission was analyzed under different growing temperatures. The results show (i) the high efficiency of Movento® to protect rapeseed and sugar beet plants against the establishment of aphid colonies, (ii) the impact of temperature on the persistence of the Movento® aphicid properties and (iii) a decrease of approximately 10% of the viral transmission on treated plants. These observations suggest a beneficial effect of Movento® on the sanitary quality of treated crops by directly reducing primary infections and indirectly altering, through aphid mortality, secondary infections on which the spread of disease within field depends. These data constitute important elements for the future development of management strategies to protect crops against aphid-transmitted viruses.

Copper use in organic agriculture in twelve European countries.

Reduction of copper-based fungicides with the final aim of phasing out has a high priority in European policy as well as in organic agriculture. Our survey aims at providing an overview of the current use of copper-based plant protection products in European organic agriculture and the need for alternatives to allow policy makers to develop strategies for a complete phasing out. Due to a lack of centralized databases on pesticide use, our survey combines expert knowledge on permitted and real copper use per crop and country with statistics on organic area. In the 12 surveyed countries, covering together 83% of the European organically managed horticultural area, we calculated approximately 3258 t copper metal per year are consumed by organic agriculture, equalling to 53% of the permitted annual dosage. This amount is split between olives (1263 t y-1, 39%), grapevine (990t y-1, 30%), and almonds (317 t y-1, 10%), followed by other crops with much smaller annual uses (<80 t y-1). Potato, usually considered a highly demanding plant for copper inputs, only uses 39 t y-1 of copper per year. In 56% of the allowed cases (countries × crops), farmers use less than half of the allowed amount, and in 27% less than a quarter, with some variability between countries. Considering the large volumes of copper used annually, replacement of copper seems only feasible if all preventive strategies from the crop protection pyramid are fully implemented and several affordable alternative plant protection products are successfully brought to the market.