Transcript abundance of heat shock protein genes confer heat tolerance in cotton (Gossypium hirsutum L.)

Abstract Monoterpenes are common constituents of essential oils produced by plants. Although it has been reported that monoterpenes enhanced the heat tolerance of plants, the mechanism has not been elucidated. Here, we tested whether 13 monoterpenes promoted the heat shock response (HSR) in Arabidopsis. To assess the HSR-inducing activity of monoterpenes, we produced transgenic Arabidopsis, which has the β-glucuronidase gene driven by the promoter of a small heat shock protein (HSP17.6C-CI) gene. Results indicated that two monocyclic and four bicyclic monoterpenes showed HSR-inducing activities using the reporter gene system. In particular, (−)-perillaldehyde, which is a monocyclic monoterpene, demonstrated the most potent HSR-inducing activity. (−)-Perillaldehyde significantly inhibited the reduction of chlorophyll content by heat shock in Arabidopsis seedlings. Our previous study indicated that chemical HSR inducers such as geldanamycin and sanguinarine inhibited the activity of plant chaperones in vitro. (−)-Perillaldehyde also inhibited chaperone activity, indicating that it might promote the expression of heat shock protein genes by inhibiting chaperones in the plant cell.

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Using double-stranded RNA to explore the role of heat shock protein genes in heat tolerance in Bemisia tabaci (Gennadius)

Journal of Experimental Biology ◽

10.1242/jeb.047415 ◽

2011 ◽

Vol 214 (5) ◽

pp. 764-769 ◽

Cited By ~ 48

Author(s):

Z.-C. Lu ◽

F.-H. Wan

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Bemisia Tabaci ◽

Heat Tolerance ◽

Double Stranded Rna ◽

Heat Shock Protein Genes

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Cloning, Expression, and Mapping of Six Low-Molecular-Weight Heat- Shock Protein Genes in Cotton

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2008.01574 ◽

2009 ◽

Vol 34 (9) ◽

pp. 1574-1580

Author(s):

Ya-Jun HE

Keyword(s):

Molecular Weight ◽

Heat Shock ◽

Heat Shock Protein ◽

Low Molecular Weight ◽

Heat Shock Protein Genes

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Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

Molecular and Cellular Biology ◽

10.1128/mcb.9.1.332 ◽

1989 ◽

Vol 9 (1) ◽

pp. 332-335 ◽

Cited By ~ 7

Author(s):

S E Kelly ◽

I L Cartwright

Keyword(s):

Drosophila Melanogaster ◽

Heat Shock ◽

Heat Shock Protein ◽

Intergenic Region ◽

Small Heat Shock Protein ◽

Dnase I ◽

Developmentally Regulated ◽

Regulatory Interactions ◽

Chromatin Architecture ◽

Heat Shock Protein Genes

Alterations in the pattern of DNase I hypersensitivity were observed on ecdysterone-stimulated transcription of Drosophila melanogaster small heat shock protein genes. Perturbations were induced near hsp27 and hsp22, coupled with an extensive domain of chromatin unfolding in the intergenic region between hsp23 and the developmentally regulated gene 1. These regions represent candidates for ecdysterone regulatory interactions.

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