Axons are the primary targets of nitric oxide mediated injury in an in vitro model of inflammatory neuropathy

2007 ◽  
pp. 87-88
Keyword(s):  
Nitric Oxide ◽  
In Vitro Model ◽  
Inflammatory Neuropathy ◽  
Vitro Model

scholarly journals In Vitro Model of Mycobacterial Growth Arrest Using Nitric Oxide with Limited Air

2008 ◽  
Vol 53 (1) ◽  
pp. 157-161 ◽  
Author(s):  
Syed Hussain ◽  
Muhammad Malik ◽  
Lanbo Shi ◽  
Maria Laura Gennaro ◽  
Karl Drlica
Keyword(s):  
Nitric Oxide ◽  
In Vitro Model ◽  
Transition Period ◽  
Time Lag ◽  
Growth Arrest ◽  
Ring Structure ◽  
Time Lags ◽  
Vitro Model ◽  
Mycobacterial Growth

ABSTRACT An in vitro model of mycobacterial growth arrest was developed using Mycobacterium bovis BCG. When an exponentially growing culture was transferred to an evacuated tube, growth continued; treatment with a source of nitric oxide (diethylenetriamine-nitric oxide adduct [DETA-NO] at 50 μM) halted growth immediately, and aeration restored growth. When the period of growth arrest exceeded 4 h, a time lag occurred before aeration could restore growth. The lag time was maximal (24 h) after 16 h of growth arrest. These time lags indicated that one transition period was required for cells to achieve full arrest of growth and another for them to recover fully from growth arrest. DETA-NO-induced growth arrest failed to protect from the lethal effects of anaerobic shock, which caused rapid lysis of both growing and growth-arrested cells. While growth arrest had little effect on the lethal action of rifampin, it eliminated isoniazid lethality. Growth arrest reduced but did not eliminate fluoroquinolone lethality. Two fluoroquinolones, moxifloxacin and gatifloxacin, were equally lethal to exponentially growing cells, but moxifloxacin was more active during growth arrest. This difference is attributed to the fluoroquinolone C-7 ring structure, the only difference between the compounds. Collectively these data characterize a new system for halting mycobacterial growth that may be useful for evaluating new antituberculosis agents.

Induction of Arginase II mRNA by Nitric Oxide Using an In Vitro Model of Gyrate Atrophy of Choroid and Retina

2011 ◽  
Vol 52 (3) ◽  
pp. 1493 ◽  
Author(s):  
Masayuki Ohnaka ◽  
Emiko Okuda-Ashitaka ◽  
Shiho Kaneko ◽  
Akira Ando ◽  
Masahide Maeda ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Vitro Model ◽  
Gyrate Atrophy ◽  
Arginase Ii ◽  
Vitro Model

scholarly journals Impaired functions of neural stem cells by abnormal nitric oxide-mediated signaling in an in vitro model of Niemann-Pick type C disease

Cell Research ◽  
2008 ◽  
Vol 18 (6) ◽  
pp. 686-694 ◽  
Author(s):  
Sun-Jung Kim ◽  
Myung-Sin Lim ◽  
Soo-Kyung Kang ◽  
Yong-Soon Lee ◽  
Kyung-Sun Kang
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vitro Model ◽  
Type C ◽  
Vitro Model ◽  
Niemann Pick

scholarly journals Biological activity of interferon gamma and lipopolysaccharide on the nitric oxide production in C6 astroglioma cells and some unexpected effects of potentization

2021 ◽  
Vol 14 (2) ◽  
pp. 23-27
Author(s):  
Clara Bonafini ◽  
Marta Marzotto ◽  
Debora Olioso ◽  
Paolo Bellavite
Keyword(s):  
Nitric Oxide ◽  
Interferon Gamma ◽  
In Vitro Model ◽  
Oxygen Free Radicals ◽  
Cell Model ◽  
Vitro Model ◽  
Oxide Synthase ◽  
Stimulation Of ◽  
Astroglioma Cells

Background: A proinflammatory environment is a hallmark of several neurodegenerative diseases where astrocyte involvement is also well established. Astrocytes and microglia in central nervous system are mainly involved in the release of cytokines, oxygen free radicals and nitric oxide (NO). Several studies on C6 astroglioma cells, a widely used in vitro model for these events, demonstrated that co-stimulation of this cell line with bacterial lipopolysaccharide (LPS) and interferon gamma (IFN-) induces a synergistic nitric oxide synthase (iNOS) expression.1 In our laboratory we are using this versatile cell model in order to carefully investigate dose-response effects of various putative agonists or inhibitors and to assess the possible changes provoked in those agents by different procedures of dilution and succussion (agitation) (potentization according to the homeopathic terminology).

The cerebrovascular response to elevated potassium – role of nitric oxide in the in vitro model of isolated rat middle cerebral arteries

2001 ◽  
Vol 306 (1-2) ◽  
pp. 61-64 ◽  
Author(s):  
Sigrid Schuh-Hofer ◽  
Elmar Lobsien ◽  
Renate Brodowsky ◽  
Johannes Vogt ◽  
Jens Peter Dreier ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Vitro Model ◽  
Cerebral Arteries ◽  
Middle Cerebral Arteries ◽  
Vitro Model ◽  
Isolated Rat

Nitric oxide donors attenuate increase in pressure drop across membrane oxygenators

Perfusion ◽  
1999 ◽  
Vol 14 (5) ◽  
pp. 331-336 ◽  
Author(s):  
F De Somer ◽  
L Foubert ◽  
E Schacht ◽  
G Van Nooten
Keyword(s):  
Nitric Oxide ◽  
Pressure Drop ◽  
In Vitro Model ◽  
Membrane Oxygenator ◽  
In Vivo Models ◽  
Initial Resistance ◽  
Membrane Oxygenators ◽  
Vitro Model

We have evaluated the effect of nitric oxide (NO) on the pressure drop across a membrane oxygenator in one in vitro model and two in vivo models (using four dogs and five pigs). In all the experiments sodium nitroprusside (SNP) was used as a NO source, whereas gaseous NO was only used in the in vitro model. The drugs were given when the pressure drop or resistance across the device increased to at least twice the baseline values. In the in vitro model, both SNP and gaseous NO decreased the pressure drop to 75% of its peak value after 10 min and to 67% after 20 min. In the dog model, resistance decreased from 390 to 153 mmHg/l/min after 5 min and to 85 mmHg/l/min after 20 min for a baseline value of 75 mmHg/l/min. The initial resistance across the membrane oxygenator in the pig model increased from 6.6 ± 1.3 to 74 ± 38 mmHg/l/min. An infusion of 10 μg/kg/min SNP reduced the resistance to 16 ± 5 mmHg/l/min.

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