Anticoagulant Citrate Phosphate Dextrose Solution

Transfusion ◽  
1961 ◽  
Vol 1 (5) ◽  
pp. 280-287 ◽  
Author(s):  
John G. Gibson ◽  
Clinton B. Gregory ◽  
Lawrence N. Button

2003 ◽  
Vol 13 (5) ◽  
pp. 614-616
Author(s):  
T. Yamada ◽  
H. Mori ◽  
M. Ueki

For the surgery of gynecological malignant tumors, a predeposit type of autologous blood transfusion has been widely used. However, using molecular biologic techniques, malignant cells have been found in the peripheral blood of cancer patients. Therefore, to evaluate the utility of presurgical blood deposits, we studied the survival of gynecological malignant cultured cells after 4°C storage in citrate-phosphate-dextrose solution. Ten cultured cell lines derived from gynecological malignant tumors were used. Mixtures of 1 × 105 cells, culture medium, and citrate-phosphate-dextrose (CPD) solution were stored at 4°C. After 0, 1, 4, 8, 15, or 22 days of storage, cells were placed in 96-well microtiter plates in culture medium with 1 × 104 cells/100μl/well. After 24 h culture in the incubator, the survival rate was calculated from the optical density by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay. More than 10% of surviving cells were seen in nine cell lines after 4 days of storage, in seven cell lines after 8 days, in three cell lines after 15 days, and in two cell lines after 22 days. Cancer cells in presurgical blood deposits may survive a 3-week storage period at 4°C in CPD solution.


Transfusion ◽  
1965 ◽  
Vol 5 (5) ◽  
pp. 427-433 ◽  
Author(s):  
Sherwin V. Kevy ◽  
John G. Gibson ◽  
Lawrence Button

1957 ◽  
Vol 28 (6) ◽  
pp. 569-578 ◽  
Author(s):  
John G. Gibson ◽  
Searle B. Rees ◽  
Thomas J. McManus ◽  
Walter A. Scheitlin

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Isabella Oliveira Barros ◽  
Rejane Santos Sousa ◽  
Marcondes Dias Tavares ◽  
Renato Otaviano Rêgo ◽  
Paulo Ricardo Firmino ◽  
...  

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.


1976 ◽  
Vol 86 (8) ◽  
pp. 1272-1279 ◽  
Author(s):  
Robert N. Miller ◽  
Robert Engelhardt ◽  
John A. Collins ◽  
Eduardo Slatopolsky ◽  
Jack H. Ladenson

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