mitochondrial redox state
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PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258833
Author(s):  
Reinier J. de Vries ◽  
Stephanie E. J. Cronin ◽  
Padraic Romfh ◽  
Casie A. Pendexter ◽  
Rohil Jain ◽  
...  

Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.



Author(s):  
Vahid Ghanbarinejad ◽  
Mohammad M. Ommati ◽  
Zhipeng Jia ◽  
Omid Farshad ◽  
Akram Jamshidzadeh ◽  
...  


2021 ◽  
Author(s):  
Nayara C. Leite ◽  
Flávia de Paula ◽  
Camila Lubaczeuski ◽  
Patricia C. Borck ◽  
Jonàs Juan-Mateu ◽  
...  

ABSTRACTChronic exercise protects pancreatic beta cells from diabetogenic stress, reducing insulin secretion through unknown mechanisms. We tested the hypothesis that the IL-6/mitochondrial STAT3 (pS-STAT3) axis plays a role in this protective effect. C57BL/6N mice were subjected to endurance training ahead of pancreatic islet isolation and functional analysis. Similar in vitro experiments were performed using insulin-producing INS-1E cells and islets from untrained mice, cultured with serum from trained animals and treated with or without an IL-6 receptor (IL-6R) inhibitor. Then, IL-6R/pS-STAT3 pathway activation and its effects on mitochondrial function and insulin secretion were assessed. Exercise-induced down-regulation of insulin secretion was prevented by inhibition of IL-6R signaling and following STAT3 knockdown. IL-6R activation promoted STAT3 phosphorylation and translocation to the mitochondria, increasing oxygen consumption. Accordingly, lower H2O2 content was reported in islets from trained mice and beta cells exposed to exercise-conditioned serum, while exposure to exogenous H2O2 blocked the down-regulatory effect of training on insulin secretion. Similar findings were observed in islets from obese-trained mice. Together, these findings suggest that the IL-6R/pS-STAT3 axis mediates exercise-induced down-regulation of insulin secretion through modulation of the mitochondrial redox state.



2021 ◽  
Vol 11 (1) ◽  
pp. 107-118
Author(s):  
Shima Mehrvar ◽  
Soudeh Mostaghimi ◽  
Farnaz H. Foomani ◽  
Betsy Abroe ◽  
Janis T. Eells ◽  
...  




2020 ◽  
Vol 1 (3) ◽  
pp. 100160
Author(s):  
Pin-Chao Liao ◽  
Emily J. Yang ◽  
Liza A. Pon


2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Allen W. Cowley ◽  
Chun Yang ◽  
Satoshi Shimada ◽  
Nadya Zheleznova ◽  
Shima Mehrvar ◽  
...  


Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 252 ◽  
Author(s):  
Matthias L. Riess ◽  
Reem Elorbany ◽  
Dorothee Weihrauch ◽  
David F. Stowe ◽  
Amadou K.S. Camara

The effect of anti-diabetic thiazolidinediones (TZDs) on contributing to heart failure and cardiac ischemia/reperfusion (IR) injury is controversial. In this study we investigated the effect of select TZDs on myocardial and mitochondrial function in Brown Norway rat isolated hearts. In a first set of experiments, the TZD rosiglitazone was given acutely before global myocardial IR, and pre- and post-IR function and infarct size were assessed. In a second set of experiments, different concentrations of rosiglitazone and pioglitazone were administered in the presence or absence of the specific PPARγ antagonist GW9662, and their effects on the mitochondrial redox state were measured by online NADH and FAD autofluorescence. The administration of rosiglitazone did not significantly affect myocardial function except for transiently increasing coronary flow, but it increased IR injury compared to the control hearts. Both TZDs resulted in dose-dependent, reversible increases in mitochondrial oxidation which was not attenuated by GW9662. Taken together, these data suggest that TZDs cause excessive mitochondrial uncoupling by a PPARγ-independent mechanism. Acute rosiglitazone administration before IR was associated with enhanced cardiac injury. If translated clinically, susceptible patients on PPARγ agonists may experience enhanced myocardial IR injury by mitochondrial dysfunction.



Author(s):  
Pin-Chao Liao ◽  
Sandra Franco-Iborra ◽  
Yi Yang ◽  
Liza A. Pon


Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 552 ◽  
Author(s):  
Reetta J. Holmila ◽  
Stephen A. Vance ◽  
S. Bruce King ◽  
Allen W. Tsang ◽  
Ravi Singh ◽  
...  

Silver nanoparticles (AgNPs) are widely used nanomaterials in both commercial and clinical biomedical applications, due to their antibacterial properties. AgNPs are also being explored for the treatment of cancer in particular in combination with ionizing radiation. In this work, we studied the effects of AgNPs and ionizing radiation on mitochondrial redox state and function in a panel of lung cell lines (A549, BEAS-2B, Calu-1 and NCI-H358). The exposure to AgNPs caused cell cycle arrest and decreased cell proliferation in A549, BEAS-2B and Calu-1, but not in NCI-H358. The mitochondrial reactive oxygen species (ROS) and protein oxidation increased in a time- and dose-dependent manner in the more sensitive cell lines with the AgNP exposure, but not in NCI-H358. While ionizing radiation also induced changes in the mitochondrial redox profiles, in general, these were not synergistic with the effects of AgNPs with the exception of NCI-H358 and only at a higher dose of radiation.



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