scholarly journals OBSERVATIONS ON THE BIOCHEMICAL EFFECTS OF TRANSFUSION OF CITRATE-PHOSPHATE-DEXTROSE STORED BLOOD IN MAN

1976 ◽  
Vol 86 (8) ◽  
pp. 1272-1279 ◽  
Author(s):  
Robert N. Miller ◽  
Robert Engelhardt ◽  
John A. Collins ◽  
Eduardo Slatopolsky ◽  
Jack H. Ladenson
Keyword(s):  
Biochemical Effects ◽  
Citrate Phosphate Dextrose ◽  
Stored Blood ◽  
Citrate Phosphate

scholarly journals Time Dependent Erythrocyte Morphometric Changes of Prolonged Stored Blood and its Effect on Target Post-transfusion Haematocrit of Splenectomised Mongrel Dogs

2021 ◽  
Author(s):  
N.H. Okereke ◽  
R.I. Udegbunam ◽  
S.O. Udegbunam ◽  
T.H. Ezeobialu ◽  
K.E. Ezenwaka
Keyword(s):  
Morphometric Parameters ◽  
Time Dependent ◽  
Intraoperative Blood Loss ◽  
Blood Storage ◽  
Mean Values ◽  
Group I ◽  
Citrate Phosphate Dextrose ◽  
Healthy Dogs ◽  
Stored Blood ◽  
Citrate Phosphate

Background: Mean values of erythrocytic morphometric parameters of very old blood and its effect on the target post-transfusion haematocrit changes of splenectomised dogs was studied. Methods: Two hundred and fifty milliliters of blood each were drawn from healthy dogs (n=6) into citrate phosphate dextrose adenine-1 anti-coagulated blood bags, preserved for 35 days for the evaluation of erythrocyte morphometric and viability parameters. Thereafter, twenty adult male splenectomised dogs were randomly assigned into 5 groups (n=3). Post-splenectomy, 4, 14, 21 and 28 day old blood (DOB) were transfused to groups II-V while group I animals were not transfused. Intraoperative blood loss was determined during the surgery while post-transfusion, animals haematocrit were assayed and used to calculate the targeted haematocrit. Result: Findings revealed irreversible progressive time dependent morphometric changes by day 14 of blood storage. Hence, it is recommended that for transfusion purposes, 4 DOB should be the hallmark as it achieved the desired haematocrit and no morphometric changes were observed from it.

scholarly journals Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1777-1781 ◽  
Author(s):  
GM Wagner ◽  
DT Chiu ◽  
JH Qju ◽  
RH Heath ◽  
BH Lubin
Keyword(s):  
Shelf Life ◽  
Strong Correlation ◽  
Exchange Chromatography ◽  
Blood Bank ◽  
Disulfide Exchange ◽  
Citrate Phosphate Dextrose ◽  
Membrane Vesiculation ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284 ◽  
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

Abstract The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals The storage of hard-packed red blood cells in citrate-phosphate- dextrose (CPD) and CPD-adenine (CPDA-1)

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 280-284
Author(s):  
E Beutler ◽  
C West
Keyword(s):  
Shelf Life ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Donor ◽  
Red Cell ◽  
Packed Red Blood Cells ◽  
Citrate Phosphate Dextrose ◽  
Storage Cells ◽  
Stored Blood ◽  
Citrate Phosphate

The preservation of red cells “hard packed” to a hematocrit of over 80% from blood collected in citrate-phosphate-dextrose (CPD) or CPD-adenine (CPDA-1) has been investigated. After 21 days of storage, cells that had been collected in CPD solution had consumed most or all of the available glucose and manifested markedly impaired viability after reinfusion into the normal donor. In contrast, red cells prepared from blood collected in CPDA-1, a medium containing supplementary adenine and an increased amount of glucose, maintained higher glucose and adenosine triphosphate levels and, in most instances, manifested satisfactory posttransfusion viability. We emphasize that in addition to providing longer shelf life of stored blood, CPDA-1 provides a better hard-packed red cell concentrate for transfusion at 21 days.

scholarly journals Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1777-1781
Author(s):  
GM Wagner ◽  
DT Chiu ◽  
JH Qju ◽  
RH Heath ◽  
BH Lubin
Keyword(s):  
Shelf Life ◽  
Strong Correlation ◽  
Exchange Chromatography ◽  
Blood Bank ◽  
Disulfide Exchange ◽  
Citrate Phosphate Dextrose ◽  
Membrane Vesiculation ◽  
Stored Blood ◽  
Citrate Phosphate

An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.

scholarly journals Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Isabella Oliveira Barros ◽  
Rejane Santos Sousa ◽  
Marcondes Dias Tavares ◽  
Renato Otaviano Rêgo ◽  
Paulo Ricardo Firmino ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Collection ◽  
Blood Gas ◽  
Blood Cell Count ◽  
Animal Blood ◽  
Equus Asinus ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

scholarly journals Anticoagulants on yield of bone marrow-derived mononuclear cells harvested from dogs

2015 ◽  
Vol 45 (11) ◽  
pp. 2044-2049
Author(s):  
Harald Fernando Vicente de Brito ◽  
Tobias Fernandes-Filho ◽  
Fernanda de Lima Brandão ◽  
Patrícia Yukiko Montaño ◽  
Luciane Maria Laskoski ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Iliac Crest ◽  
Mononuclear Cells ◽  
Cd44 Expression ◽  
Citrate Phosphate Dextrose ◽  
Healthy Dogs ◽  
Citrate Phosphate ◽  
Paired T Test

Cell therapy with bone marrow-derived mononuclear cells is an alternative to therapy with mesenchymal stem cell cultures. The aim of the present research was the comparison of the yield of bone marrow-derived mononuclear cells harvested from dogs with two different anticoagulants. Bone marrow was harvested from the iliac crest of five healthy dogs aged between 15 and 30 months, and the effect of two anticoagulant solutions, CPDA-1 (citrate phosphate dextrose adenine-1) and heparin, on the isolation of mononuclear cells was compared. Mononuclear cells were isolated in a density gradient and stained for CD9 and CD44 for characterization by flow cytometry. Means were compared using Student's paired t-test. Samples harvested with CPDA-1 yielded an average of 5.16x106 (±1.76x106) to 20.20x106 (±1.55x106) mononuclear cells/mL, whereas the yield of samples harvested with heparin varied between 4.56x106 (±0.69x106) and 24.30x106 (±2.12x106) mononuclear cells mL-1. By flow cytometry, mean percentage of double-stained cells varied from 1.96% (±0.64%) to 5.01% (±0.73%) for CPDA-1 and from 2.23% (±0.70%) to 7.27% (±0.97%) for heparin. No significant statistical differences were observed on yield or CD9 and CD44 expression. Further studies are recommended to assess efficacy of CPDA on mononuclear cell isolation.

Flow-dependent influence of high-O2-affinity erythrocytes on peak VO2 in exercising muscle in situ

1996 ◽  
Vol 80 (3) ◽  
pp. 832-838 ◽  
Author(s):  
H. Kohzuki ◽  
Y. Enoki ◽  
K. Matsumura ◽  
S. Sakata ◽  
S. Shimizu
Keyword(s):  
O2 Uptake ◽  
High Affinity ◽  
Staircase Effect ◽  
Citrate Phosphate Dextrose ◽  
High O2 ◽  
Rate Of Decline ◽  
O2 Delivery ◽  
Citrate Phosphate ◽  
Peak Value

To evaluate the influence of a high-O2 affinity of the erythrocyte and of flow rate on muscle's ability to extract O2 and develop force, we perfused dog gastrocnemius contracting isometrically at 4 Hz with normal-O2-affinity perfusate or high-O2-affinity perfusate at high and moderate flows (200 and 100 ml . min-1 . 100g-1, respectively). High-O2-affinity perfusate was prepared by incubating human citrate-phosphate-dextrose-stored erythrocytes with buffered saline containing cyanate (4 degrees C, 18 h) and normal-affinity perfusate by storing 2,3-diphosphoglycerate-rejuvenated erythrocytes in the same solution without cyanate. PO2 when blood is half oxygenated was 30.6 Torr for normal perfusate and 18.1 Torr for high-affinity perfusate. During 4-Hz stimulation, the tension developed by the muscle increased incrementally (positive staircase) to reach a peak value after 1.2-1.6 min for the normal perfusate and 0.6-0.7 min for the high-affinity perfusate (P < 0.05). The rate of decline during the early fatigue (measured from the onset of tension decline to 3 min) with high-affinity perfusate was significantly faster than it was with normal perfusate (P < 0.05). These findings suggest that both the staircase effect and the early fatigue are related to O2 availability, which is restricted when erythrocytes have a high O2 affinity. The peak O2 uptake values measured at 3 and 5 min were significantly lower (by 14-24%) with high-affinity perfusate than with normal perfusate at a given level of O2 delivery (arterial O2 content x flow) (P < 0.05). PO2 of venous effluent was proportionally related to peak O2 uptake. The present results indicate that neither blood flow nor O2 delivery is the sole determinant of the muscle's ability to extract O2.

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