scholarly journals Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Isabella Oliveira Barros ◽  
Rejane Santos Sousa ◽  
Marcondes Dias Tavares ◽  
Renato Otaviano Rêgo ◽  
Paulo Ricardo Firmino ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Collection ◽  
Blood Gas ◽  
Blood Cell Count ◽  
Animal Blood ◽  
Equus Asinus ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

scholarly journals Hematological, biochemical, and blood gas alterations of goat whole blood stored in CPDA-1 and CPD/SAG-M plastic bags

2019 ◽  
Vol 49 (10) ◽  
Author(s):  
Marcondes Dias Tavares ◽  
Isabella de Oliveira Barros ◽  
Rejane dos Santos Sousa ◽  
Paulo Ricardo Firmino ◽  
Jucelio da Silva Gameleira ◽  
...  
Keyword(s):  
Cell Count ◽  
Whole Blood ◽  
Hemoglobin Concentration ◽  
Blood Gas ◽  
Male Adult ◽  
Plasma Hemoglobin ◽  
Total Hemoglobin ◽  
Plastic Bags ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

ABSTRACT: The aim of this study was to evaluate the hematological, biochemical, and blood gas alterations of goat whole blood stored in different blood bags. Seven male, adult, crossbreed goats were used, weighing 62±1.8 kg. Nine hundred milliliters of whole blood from each animal was collected and stored in blood bags (450 ml in each), CPDA-1 (citrate phosphate dextrose-adenine) and CPD/SAG-M (citrate phosphate dextrose with saline-glucose-mannitol) as additive solutions, and kept refrigerated (2-4 ºC) for 42 days. Blood samples were collected from the plastic bags at baseline (T0) and after seven, 14, 21,28, 35, and 42 days for hematological, biochemical, blood gas, and microbiological evaluations. Free hemoglobin, degree of hemolysis, lactate, and pO2were increased in both bags, whereas hydrogen potential (pH) and the total hemoglobin concentration decreased overtime(P<0.05). The red blood cell count, glucose, sodium, and potassium remained stable, compared to the baseline. The CPD/SAG-M bag presented a lower red cell count, globular volume, total hemoglobin, and sodium, and a higher degree of hemolysis and plasma hemoglobin, compared with the CPDA-1 bag. The whole goat blood remained viable for therapeutic use; although, there were some important changes in the variables of the 42-day stored blood in relation to fresh blood (T0). We concluded that the CPDA-1 bag is more suitable for use in the storage of goat blood because of its lower commercial value.

scholarly journals PSIV-27 Peripheral complete blood cell count results in pigs are impacted by gender, sow parity, and farrowing season

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 291-292
Author(s):  
Elle Rottman ◽  
Alisun N Watson ◽  
Catherine Buck ◽  
Tsungcheng Tsai ◽  
Jeffery J Chewning ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Mean Corpuscular Hemoglobin ◽  
Complete Blood Cell Count ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Cell Counts

Abstract Complete blood cell counts have been used as a diagnostic tool across many animal species including swine. To investigate the factors that cause variation in complete blood cell count results, a total of 2,284 whole blood samples were collected from 2012 to 2019 in preweaning piglets (n = 518), nursery pigs (n = 1,704), and grower pigs (n = 60). Whole blood was collected into K2EDTA blood collection tubes and assayed using an automatic hematologic analyzer within 6 hours of collection. Data were analyzed by Mixed procedure of SAS with gender, parity group, and farrowing season as fixed effects. Body weight and age of pigs served as covariances. Farrowing season was grouped into summer (born during May to October) or winter (or November to April). Pigs that were born from first, second, and third parity, and four and above parity sows were assorted into parity group 1, 2 to 3, and 4+, respectively. Barrows had a greater concentration of total white blood cells (P &lt; 0.01), lymphocytes (P &lt; 0.01), and neutrophils (P &lt; 0.01) compared to gilts. Barrows had lower mean corpuscular volume (P = 0.03), mean corpuscular hemoglobin (P &lt; 0.01), and mean corpuscular hemoglobin concentration (P = 0.02) compared to gilts. Pigs that were farrowed in the winter season had a greater concentration of white blood cells (P = 0.01), neutrophils (P = 0.01), and the percentage of neutrophils (P = 0.03), but were lower in the percentage of lymphocytes (P = 0.03) compared to pigs farrowed during summer. Pigs born to parity four and above sows obtained a greater lymphocyte count (P = 0.01), percentage of neutrophils (P = 0.02), and percentage of lymphocytes (P = 0.01). We concluded that peripheral complete blood cells count results were affected by gender, farrowing season, and sow parity.

Myeloproliferative Neoplasm Resembling Polycythemia Vera In a Patient with t(2;11)(p21;q23) Translocation and Persistently Elevated MiR-125b-1

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 5067-5067
Author(s):  
Stanley R. McCormick ◽  
Craig W. S. Howe ◽  
Pierre Brousset ◽  
Anil K. Tadavarthy ◽  
Cathy Quelen ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Polycythemia Vera ◽  
White Blood Cell Count ◽  
White Blood Cell ◽  
Whole Blood ◽  
Myeloid Leukemia ◽  
Myeloproliferative Neoplasm ◽  
Blood Cell Count ◽  
Qrt Pcr

Abstract Abstract 5067 Background: Hematologic neoplasms associated with the chromosomal translocation t(2;11)(p21;q23) form a distinct genetic entity with diverse manifestations, and have been strongly linked with up-regulation of the microRNA miR-125b-1 (Bousquet et al, J Exp Med, 2008). Of 41 patients with myeloid malignancies and t(2;11)(p21;23) reported in the world's literature, all but 2 were cases of acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS), the latter cases frequently high-grade MDS transforming to AML. In 20 of 41 cases deletion of 5q was detected as a secondary cytogenetic abnormality. Only two cases of chronic myeloproliferative disorders associated with t(2;11)(p21;23) have been reported: one of chronic myeloid leukemia with a secondary t(9;22) (Ph) translocation (Royer-Pokora et al, Leukemia, 2003) and one of polycythemia vera (Acar et al, Amer J Hematol, 2006). We encountered a patient with a mixed myelodysplastic/myeloproliferative neoplasm clinically resembling polycythemia vera that was associated with t(2;11)(p21;q23) who was found to have persistent elevation in the blood of the microRNA miR-125b-1. Case Report: A 52 year-old male presented with upper extremity pain and headache. A CBC revealed the white blood cell count was 12.5 ×103/uL (55% neutrophils, 12% lymphocytes, 8% monocytes, 5% eosinophils, 21% basophils), red blood cell count 6.62 × 106/uL, hemoglobin 22.4 g/dL, hematocrit 68.1%, MCV 103 fL, and platelets 112 × 103/uL. Bone marrow aspiration and biopsy revealed a markedly hypercellular marrow (100%) with 1+ reticulin fibrosis, mild trilineal dysplastic morphology, and a blast count of 0.8%. Cytogenetic studies disclosed 46, XY, t(2;11)(p21;q23) [4]/46, idem, del (5)(q15q31) [4]/46, XY, del (5) (q13q31) [2]/46, XY[10]. FISH studies were negative for BCR/ABL1 fusion and MLL rearrangement. Molecular analysis for the JAK2V617F allele was negative. A diagnosis of mixed myelodysplastic/myeloproliferative neoplasm, unclassified was made (WHO 2008). The patient was begun on hydroxyurea, 500 mg daily. Four months after diagnosis the blood counts were within normal range, at which time quantitative real-time PCR (qRT-PCR; Bousquet et al, J Exp Med, 2008) performed on a sample of whole blood revealed a twenty-fold elevation of the microRNA species miR-125b-1, compared to healthy donors (n=3), and JAK2V617F-negative (n=3) and JAK2V617F-positive (n=2) polycythemia vera controls. Repeat qRT-PCR performed on whole blood 15 months after diagnosis confirmed persistently elevated miR-125b-1, nearly 200-fold above healthy donor controls (n=3). 18 months after diagnosis the patient remains healthy, with normal blood counts on 500 mg of hydroxyurea daily (white blood cell count 5.54 ×103/uL, red cell count 5.35 × 106/uL, hemoglobin 13.7 g/dL, hematocrit 47.2%, MCV 88.2, platelets 132.0 × 103/uL). Conclusion: This case expands the clinical-pathologic spectrum of hematologic malignancies associated with t(2;11)(p21;23), and confirms the link between t(2;11)(p21;23) and up-regulation of miR-125b-1. Translocation t(2;11)(p21;23) with miR-125b-1 up-regulation should be considered as a rare possibility in the differential diagnosis of JAK2V617F-negative polycythemia vera. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Stability of Hematological Parameters in Woodland Caribou (Rangifer tarandus caribou) Blood Stored at 4°C

Rangifer ◽  
1996 ◽  
Vol 16 (4) ◽  
pp. 297
Author(s):  
G. Piat ◽  
S. Semalulu ◽  
Z. Florence ◽  
J. Nolan
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Rangifer Tarandus ◽  
Hematological Parameters ◽  
Blood Collection ◽  
Woodland Caribou ◽  
Blood Cell Count ◽  
Mean Cell Volume ◽  
Distribution Width ◽  
Complete Blood Counts

Eighteen free-ranging female woodland caribou were captured in northern Alberta in January and February 1993. Blood was collected into ethylenediaminetetraacetate (EDTA) tubes which were packaged in coolers containing ice packs, and transported to the laboratory where they arrived within 48 hrs of collection. Complete blood counts (CBC) were performed on five consecutive days to assess the stability of hematological parameters. Average values of hematocrit (HCT), mean cell hemoglobin (MCH), mean cell volume (MCV), red cell distribution width (P-J3W), white blood cell count (WBC), and red blood cell count (RJ3C) remained stable with no statistically significant changes occurring during 5 days of post-collection storage at 4&deg;C. Mean PvBC values exhibited significant differences (p&lt;0.05) between geographic locations. Mean platelet volume (MPV) increased significantly (p&lt;0.001) with storage time, while platelet (PLT) values decreased (p&lt;0.001) over time and were significantly different (p&lt;0.01) between locations. For optimal hematological results, it is recommended that sample analysis be performed within 24 hours of blood collection; however, if caribou blood samples are properly stored at 4&deg;C, useful information may be obtained from stable parameters up to 5 days following collection.

scholarly journals AIDeveloper: deep learning image classification in life science and beyond

2020 ◽  
Author(s):  
Martin Kräter ◽  
Shada Abuhattum ◽  
Despina Soteriou ◽  
Angela Jacobi ◽  
Thomas Krüger ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Image Classification ◽  
Whole Blood ◽  
Classification Performance ◽  
Neural Nets ◽  
Neural Net ◽  
Label Free ◽  
Blood Cell Count ◽  
Free Classification

AbstractPublications on artificial intelligence (AI)-based image analysis have increased drastically in recent years. However, all applications use individual solutions highly specialized for a particular task. Here, we present an easy-to-use, adaptable, open source software, called AIDeveloper (AID) to train neural nets (NN) for image classification without the need for programming. The software provides a variety of NN-architectures that can be simply selected for training. AID allows the user to apply trained models on new data, obtain metrics for classification performance, and export final models to different formats. The working principles of AID are first illustrated by training a convolutional neural net (CNN) on a large dataset consisting of images of different objects (CIFAR-10). We further explore the potential of AID by training a model to distinguish areas of differentiated and non-differentiated mesenchymal stem cells (MSCs) in culture. Additionally, we compare a conventional clinical whole blood cell count with a whole blood cell count performed by an NN-trained, using a dataset of more than 1.2 million images obtained by real-time deformability cytometry, delivering comparable results. Finally, we demonstrate how AID can be used for label-free classification of B- and T-cells derived from human blood, which currently requires costly and time-consuming sample preparation. Thus, AID can empower anyone to develop, train, and apply NNs for image classification. Moreover, models can be generated by non-programmers, exported, and used on different devices, which allows for an interdisciplinary use.

scholarly journals Erythrocyte alterations of the laying hens subjected to thermal stress

2021 ◽  
pp. 47911-47913
Keyword(s):  
Heat Stress ◽  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Laying Hens ◽  
Control Group ◽  
Blood Collection ◽  
Blood Cell Count ◽  
Significant Difference ◽  
Red Blood Cell Count

The aim of this study was to identify erythrocyte changes resulting from heat stress in relation to different times of blood collection throughout the day. The experiment was carried out with 39 Rhode Island Red hens, in the initial laying phase, receiving standardized ad libitum feeding. The birds were placed in an environment of 20m2, with controlled temperature, divided into 2 distinct groups: Control Group with 12 animals at 16°C and Experimental Group with 27 animals at 30°C. Venipuncture took place at 7:00 am, 11:00 am and 3:00 pm. Blood was stored in an EDTA tube, aiming at cell preservation for further analysis. Total red blood cell count, hematimetry and erythroid morphometry were performed. After evaluating the erythroid and hematimetric parameters of the 39 birds, a negative correlation of heat stress was observed when comparing the different times of blood collection throughout the day. For morphometric correlation, there was a significant difference (P>0.01) at 3:00 pm. When evaluating the results between the times of blood collection from the same animal, it was observed that 55.5% reduced the hematocrit, while the total red blood cell count was reduced by 51.8%. It is essential that there is a systematization of time in the collection of blood from laying hens.

Cigarette Smoking Increases Thromboxane A2 Formation without Affecting Platelet Survival in Young Healthy Females

1992 ◽  
Vol 68 (05) ◽  
pp. 583-588 ◽  
Author(s):  
Annika Dotevall ◽  
Christina Rångemark ◽  
Elsa Eriksson ◽  
Jack Kutti ◽  
Hans Wadenvik ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Cigarette Smoking ◽  
Blood Cell ◽  
Urinary Excretion ◽  
Life Span ◽  
Cell Count ◽  
Thromboxane A2 ◽  
Blood Cell Count ◽  
Platelet Activity ◽  
Pai 1

SummarySmoking is a risk factor for the development of atherosclerotic cardiovascular disease, in men as well as in women. An increased urinary excretion of the thromboxane metabolite 2,3-dinor-thromboxane B2 (Tx-M) has been observed in smokers of both genders, suggesting that cigarette smoking may facilitate cardiovascular disease via an action on the platelets. The present study addressed the hypothesis that the increased Tx-M excretion in female smokers reflects a true facilitation of platelet reactivity in vivo, rather than an increased destruction of the platelets. In healthy female volunteers (aged 20–46 years, 18 smokers and 17 non-smokers) platelet life-span and indices of platelet activity were determined, together with plasma levels of plasminogen activator inhibitor-1 (PAI-1), fibrinogen, peripheral blood cell counts and hematocrit. The urinary excretion of Tx-M was higher in smokers than in non-smokers (361 vs. 204 pg/mg creatinine, respectively, p <0.05), while plasma and urinary β-thromboglobulin, plasma platelet factor 4, platelet mean life-span and platelet production rate did not differ between the groups. PAI-1 activity, white blood cell count and hematocrit were higher in smokers than in non-smokers (p <0.05). These data indicate that smoking facilitates platelet formation of thromboxane A2 without affecting platelet survival; i.e. it increases the activity of platelets without affecting their viability to a measurable extent. Such an increase in platelet activity, operating in parallel to a reduced fibrinolytic activity and a higher hematocrit and white blood cell count, may play an etiological role in smoking-induced cardiovascular disease in women.

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