Comparison of the Modifying Action of Astaxanthin on the Development of Radiation-Induced Chromosome Instability in Human Blood Lymphocytes Irradiated in vitro at Different Cell Cycle Stages

2018 ◽  
Vol 52 (5) ◽  
pp. 368-373 ◽  
Author(s):  
D. A. Kurinnyi ◽  
S. R. Rushkovskyi ◽  
O. M. Demchenko ◽  
O. B. Dybska ◽  
M. A. Pilinska
2005 ◽  
Vol 20 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Nagarajan Rajendra Prasad ◽  
Thirunavukkarasu Mahesh ◽  
Venugopal Padmanabhan Menon ◽  
R.K Jeevanram ◽  
Kodukkur Viswanathan Pugalendi

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Mona A.M. Abo-Zeid ◽  
Thomas Liehr ◽  
Amira M. Gamal-Eldeen ◽  
Mahmoud Zawrah ◽  
Mostafa Ali ◽  
...  

AbstractGold nanoparticles (GNPs) are intended to be used in nanomedicine. Due to nanotechnology innovation GNPs of variable sizes and in different shapes including rods, spheres, cubes, etc., can easily be produced. The aim of the present studies was to evaluate the cyto-and genotoxicity inducible by different shaped GNPs on normal human peripheral blood lymphocytes.Four different shapes of GNPs including big rod GNPs (BR-GNPs, 50 nm), small rod GNPs (SR-GNPs, 30 nm), sphere GNPs (S-GNPs, 15 nm) and semi-cube GNPs (SC-GNPs, 15 nm) were studied. Cultured human blood lymphocytes were treated with different concentrations of these GNPs for 24 h in vitro. Cytotoxicity was evaluated based on the mitotic index (MI), while genotoxicity was studied by an interphase-fluorescence in situ hybridization (I-FISH) assay. The following genes were studied in I-FISH:The lowest concentration of BR-GNPs neither had an effect mitotic activity nor enhanced gain or loss of examined gene signals in a significant manner with I-FSH. Other concentrations of BR-GNPs, SR-GNPs, S-GNPs and SC-GNPs with all concentrations inhibited the mitotic activity of the cells and reduced the cell proliferation highly significantly. The different types of GNPs initiated the duplication ofGNPs at high concentration can reduce the cell proliferation and induce DNA damage. Low concentration of rod-shaped GNPs at 50 nm was safe on human lymphocytes. Further research studies are required to optimize the concentration, shape and size of GNPs before using them in nanomedicine.


Sign in / Sign up

Export Citation Format

Share Document