Mechanisms of Resistance to Clinically Significant Antibiotics of Bacterial Strains of the Genus Bacillus Isolated from Samples from the International Space Station

Background: While significant attention has been paid to the potential risk of pathogenic microbes aboard crewed spacecraft, much less has focused on the non-pathogenic microbes in these habitats. Preliminary work has demonstrated that the interior of the International Space Station (ISS) has a microbial community resembling those of built environments on earth. Here we report results of sending 48 bacterial strains, collected from built environments on earth, for a growth experiment on the ISS. This project was a component of Project MERCCURI (Microbial Ecology Research Combining Citizen and University Researchers on ISS). Results: Of the 48 strains sent to the ISS, 45 of them showed similar growth in space and on earth. The vast majority of species tested in this experiment have also been found in culture-independent surveys of the ISS. Only one bacterial strain that avoided contamination showed significantly different growth in space. Bacillus safensis JPL-MERTA-8-2 grew 60% better in space than on earth. Conclusions: The majority of bacteria tested were not affected by conditions aboard the ISS in this experiment (e.g., microgravity, cosmic radiation). Further work on Bacillus safensis could lead to interesting insights on why this bacteria grew so much better in space.

Download Full-text

Growth of 48 built environment bacterial isolates on board the International Space Station (ISS)

PeerJ ◽

10.7717/peerj.1842 ◽

2016 ◽

Vol 4 ◽

pp. e1842 ◽

Cited By ~ 20

Author(s):

David A. Coil ◽

Russell Y. Neches ◽

Jenna M. Lang ◽

Wendy E. Brown ◽

Mark Severance ◽

...

Keyword(s):

International Space Station ◽

Space Station ◽

Built Environments ◽

Bacterial Strains ◽

International Space ◽

Growth Measurement ◽

Bacillus Safensis ◽

Culture Independent ◽

Pathogenic Microbes ◽

Similar Growth

Background.While significant attention has been paid to the potential risk of pathogenic microbes aboard crewed spacecraft, the non-pathogenic microbes in these habitats have received less consideration. Preliminary work has demonstrated that the interior of the International Space Station (ISS) has a microbial community resembling those of built environments on Earth. Here we report the results of sending 48 bacterial strains, collected from built environments on Earth, for a growth experiment on the ISS. This project was a component of Project MERCCURI (Microbial Ecology Research Combining Citizen and University Researchers on ISS).Results.Of the 48 strains sent to the ISS, 45 of them showed similar growth in space and on Earth using a relative growth measurement adapted for microgravity. The vast majority of species tested in this experiment have also been found in culture-independent surveys of the ISS. Only one bacterial strain showed significantly different growth in space.Bacillus safensisJPL-MERTA-8-2 grew 60% better in space than on Earth.Conclusions.The majority of bacteria tested were not affected by conditions aboard the ISS in this experiment (e.g., microgravity, cosmic radiation). Further work onBacillus safensiscould lead to interesting insights on why this strain grew so much better in space.

Download Full-text

Draft Genome Sequences of Various Bacterial Phyla Isolated from the International Space Station

Microbiology Resource Announcements ◽

10.1128/mra.00214-21 ◽

2021 ◽

Vol 10 (17) ◽

Author(s):

Anna C. Simpson ◽

Camilla Urbaniak ◽

Nitin K. Singh ◽

Jason M. Wood ◽

Marilyne Debieu ◽

...

Keyword(s):

International Space Station ◽

Draft Genome ◽

Space Station ◽

Whole Genome ◽

Bacterial Strains ◽

Genome Sequences ◽

International Space ◽

Bacterial Phyla

ABSTRACT Whole-genome sequences were generated from 96 bacterial strains of 14 species that were isolated from International Space Station surfaces during the Microbial Tracking 2 study. Continued characterization of this closed habitat's microbiome enables tracking of the spread and evolution of secondary pathogens, which is vital for astronaut health.

Download Full-text

Draft Genome Sequences of Klebsiella Species Isolated from the International Space Station

Microbiology Resource Announcements ◽

10.1128/mra.00923-20 ◽

2020 ◽

Vol 9 (42) ◽

Author(s):

Samuel A. Solomon ◽

Achintya R. Bharadwaj ◽

Nitin K. Singh ◽

Jason M. Wood ◽

Marilyne Debieu ◽

...

Keyword(s):

International Space Station ◽

Draft Genome ◽

Space Station ◽

Comparative Genomic ◽

Whole Genome ◽

Sequencing Analysis ◽

Bacterial Strains ◽

Genome Sequences ◽

Klebsiella Species ◽

International Space

ABSTRACT Isolated across four locations aboard the International Space Station (ISS), 10 bacterial strains were compared using whole-genome sequencing analysis and were phylogenetically identified as Klebsiella. The whole-genome sequences will aid in comparative genomic studies of ISS Klebsiella strains with Earth counterparts to gain insight into their adaptation to space conditions.

Download Full-text

Diversity of bacteria of the genus Bacillus on board of international space station

Doklady Biochemistry and Biophysics ◽

10.1134/s1607672915060010 ◽

2015 ◽

Vol 465 (1) ◽

pp. 347-350 ◽

Cited By ~ 4

Author(s):

T. A. Alekhova ◽

L. M. Zakharchuk ◽

N. Yu. Tatarinova ◽

V. V. Kadnikov ◽

A. V. Mardanov ◽

...

Keyword(s):

International Space Station ◽

Space Station ◽

Genus Bacillus ◽

International Space

Download Full-text

Growth of 48 built environment bacterial isolates on board the International Space Station (ISS)

10.7287/peerj.preprints.1654v1 ◽

2016 ◽

Cited By ~ 2

Author(s):

David A Coil ◽

Russell Y Neches ◽

Jenna M Lang ◽

Wendy E Brown ◽

Mark Severance ◽

...

Keyword(s):

International Space Station ◽

Space Station ◽

Built Environments ◽

Bacterial Strains ◽

International Space ◽

Bacillus Safensis ◽

Culture Independent ◽

Pathogenic Microbes ◽

Similar Growth ◽

Significant Attention

Background: While significant attention has been paid to the potential risk of pathogenic microbes aboard crewed spacecraft, much less has focused on the non-pathogenic microbes in these habitats. Preliminary work has demonstrated that the interior of the International Space Station (ISS) has a microbial community resembling those of built environments on earth. Here we report results of sending 48 bacterial strains, collected from built environments on earth, for a growth experiment on the ISS. This project was a component of Project MERCCURI (Microbial Ecology Research Combining Citizen and University Researchers on ISS). Results: Of the 48 strains sent to the ISS, 45 of them showed similar growth in space and on earth. The vast majority of species tested in this experiment have also been found in culture-independent surveys of the ISS. Only one bacterial strain that avoided contamination showed significantly different growth in space. Bacillus safensis JPL-MERTA-8-2 grew 60% better in space than on earth. Conclusions: The majority of bacteria tested were not affected by conditions aboard the ISS in this experiment (e.g., microgravity, cosmic radiation). Further work on Bacillus safensis could lead to interesting insights on why this bacteria grew so much better in space.

Download Full-text