Determination of a Microbial Cells in Their Interaction with Phage Mini-Antibodies by the Sensor Based on a PZT Resonator with a Lateral Electric Field

2021 ◽  
Vol 85 (6) ◽  
pp. 599-602
Author(s):  
I. A. Borodina ◽  
B. D. Zaitsev ◽  
A. A. Teplykh ◽  
A. K. M. Alsowaidi ◽  
O. S. Larionova ◽  
...  
Microbiology ◽  
2013 ◽  
Vol 82 (2) ◽  
pp. 215-223 ◽  
Author(s):  
O. I. Guliy ◽  
B. D. Zaitsev ◽  
I. E. Kuznetsova ◽  
A. M. Shikhabudinov ◽  
L. Yu. Matora ◽  
...  

Sensors ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 3003
Author(s):  
Irina Borodina ◽  
Boris Zaitsev ◽  
Andrey Teplykh ◽  
Gennady Burygin ◽  
Olga Guliy

A biological sensor for detection and identification of bacterial cells, including a resonator with a lateral electric field based on PZT ceramics was experimentally investigated. For bacterial immunodetection the frequency dependencies of the electric impedance of the sensor with a suspension of microbial cells were measured before and after adding the specific antibodies. It was found that the addition of specific antibodies to a suspension of microbial cells led to a significant change in these frequency dependencies due to the increase in the conductivity of suspension. The analysis of microbial cells was carried out in aqueous solutions with a conductivity of 4.5–1000 μS/cm, as well as in the tap and drinking water. The detection limit of microbial cells was found to be 103 cells/mL and the analysis time did not exceed 4 min. Experiments with non-specific antibodies were also carried out and it was shown that their addition to the cell suspension did not lead to a change in the analytical signal of the sensor. This confirms the ability to not only detect, but also identify bacterial cells in suspensions.


2015 ◽  
Vol 74 ◽  
pp. 421-425 ◽  
Author(s):  
M.G. Barseghyan ◽  
H.M. Baghramyan ◽  
D. Laroze ◽  
J. Bragard ◽  
A.A. Kirakosyan

1995 ◽  
Vol 244 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
G. Bazalgette ◽  
R. White ◽  
J.C. Loison ◽  
G. Trénec ◽  
J. Vigué

1997 ◽  
Vol 488 ◽  
Author(s):  
S. Grossmann ◽  
T. Weyrauch ◽  
W. Haase

AbstractWe report on a method to investigate the inhomogeneous distribution of an electric dc field in multilayer polymer stacks. In situ electroabsorption (EA) measurements are applied in order to estimate the local electric fields in double layer polymer films. The observed time dependent behaviour is compared with a model equivalent circuit. The results indicate that besides the relation of ohmic resistivities and capacities of the different polymer layers in the investigated systems also the influence of the electric properties of polymer/electrode and polymer/polymer interfaces must be considered.


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