Phylogenetic analysis of a section of the provirus pol gene of bovine leukemia virus isolates from animals in various regions of the Russian Federation

2011 ◽  
Vol 37 (6) ◽  
pp. 520-523
Author(s):  
N. G. Kozyreva ◽  
M. I. Gulyukin ◽  
L. A. Ivanova ◽  
A. S. Malogolovkin ◽  
A. I. Klimenko ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Russian Federation ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
The Russian Federation ◽  
Virus Isolates

scholarly journals Revisiting the Issue of the Molecular-genetic Structure of the Causative Agent of the Bovine Leukemia Virus in the Russian Federation

2016 ◽  
Vol 9 (42) ◽  
Author(s):  
Irina Mikhailovna Donnik ◽  
Maksim Valeryevich Petropavlovsky ◽  
Anna Sergeevna Krivonogova ◽  
Irina Alekseevna Shkuratova ◽  
Marzena Rola-Łuszczak ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Russian Federation ◽  
Causative Agent ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
The Russian Federation

scholarly journals Comparative molecular and genetic characterization of rabies viruses (Rabies lyssavirus, Lyssavirus, Rhabdoviridae) circulated in the Russian Federation in 1985–2016

2020 ◽  
Vol 65 (1) ◽  
pp. 41-48
Author(s):  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
M. A. Losich ◽  
A. L. Elakov ◽  
A. M. Gulyukin ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Epidemiology ◽  
Rabies Virus ◽  
Russian Federation ◽  
Amino Acid Sequences ◽  
Phylogenetic Study ◽  
Human Populations ◽  
Human Beings ◽  
The Russian Federation ◽  
Virus Isolates

Introduction. Rabies caused by the neurotropic virus of the genus Lyssavirus, Rhabdoviridae family, which infects all warm-blooded vertebrates including human beings. The homology level of the amino acid sequences for Lyssaviruses nucleoprotein reaches 78–93%.Aim – study the genetic diversity and molecular epidemiology of Lyssaviruses circulated in the Russian Federation in 1985–2016.Material and methods. 54 isolates of rabies virus isolated from animals, and 2 isolates from humans, 4 vaccine strains of rabies virus: RV-97, ERA, Shchelkovo 51, ERAG333 used in phylogenetic study. Phylogenetic analysis was performed using Genbank data on genome fragments of 73 rabies virus isolates and 9 EBLV-1 isolates. DNASTAR V.3.12, Bio Edit 7.0.4.1 and MEGA v.10.0.5, Primer Premier 5 programs have been used.Results. Comparative molecular genetic analysis of genomes fragments of 130 Lissaviruses, isolated on the territory of the RF, Ukraine in 1985-2016, vaccine strains of rabies virus, showed their distribution by geographical feature. Comparison of the nucleoprotein fragments of the rabies virus isolates with vaccine strains revealed 4 marker mutations: V56I (Eurasian group), L/V95W (Central group), D101N/S/T, and N/G106D. Phylogenetic analysis of the isolate «Juli», isolated from a human bitten by a bat proved his belonging to the European Bat lyssavirus-1a.Discussion. Study of the molecular epidemiology of rabies within the Russian Federation allows for the genotyping of the viruses and helps to study the hidden mechanisms of rabies infection in animal and human populations, and to characterize vaccine strains, including during oral vaccination.Conclusion. Further study of the molecular epidemiology of rabies within the Russian Federation and the countries bordering it is important.

scholarly journals Sequencing and phylogenetic analysis of the gp51 gene from Korean bovine leukemia virus isolates

2015 ◽  
Vol 12 (1) ◽  
Author(s):  
EunJung Lee ◽  
Eun-Ju Kim ◽  
Ha-Kyung Joung ◽  
Bo-Hye Kim ◽  
Jae-Young Song ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Isolates

scholarly journals Genetic analysis of nucleotide sequences of neuraminidase gene of highly pathogenic avian influenza A/H5N8 virus isolates recovered in the Russian Federation in 2020

2021 ◽  
pp. 301-307
Author(s):  
P. B. Akshalova ◽  
N. G. Zinyakov ◽  
A. V. Andriyasov ◽  
P. D. Zhestkov ◽  
Z. B. Nikonova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Russian Federation ◽  
Highly Pathogenic Avian Influenza ◽  
Nucleotide Sequences ◽  
Highly Pathogenic ◽  
Pathogenic Avian Influenza ◽  
The Russian Federation ◽  
Virus Isolates

Avian influenza is a highly dangerous viral disease that causes huge economic damage to poultry farming. Currently, highly virulent influenza virus with N8 neur- aminidase subtype is quite often detected in populations of domestic and wild birds in various countries of the world. The article provides data on complete nucleotide sequences of the neuraminidase gene of highly pathogenic avian influenza virus isolates recovered in the second half of 2020 from pathological material received from four regions of the Russian Federation. The conducted research showed that the subtype of the isolated virus was N8. According to the phylogenetic analysis, isolates of N8 virus belong to group 8C.4. During the phylogenetic analysis of the neuraminidase, we also took into account data on hemagglutinin classification, according to which H5N8 virus isolates belong to a widespread clade 2.3.4.4. Viruses of the clade were first registered in 2010 in China and they have been circulating up to now. The paper also provides data of a comparative analysis of nucleotide sequences of the studied isolates and the isolates from the international GenBank and GISAID databases, recovered in other countries from 2007 to 2020. During the analysis of the amino acid sequence of the studied isolates, no substitutions were found in the positions that affect resistance to neuraminidase inhibitors. The complete nucleotide sequences of the neuraminidase gene of the avian influenza virus subtype N8 (isolates A/domestic goose/OMSK/1521-1/2020, A/duck/Chelyabinsk/1207-1/2020, A/duck/Saratov/1578-2/2020, A/goose/Tatarstan/1730-2/2020) are published in the international GenBank and GISAID databases. Based on the analysis of the nucleotide sequences of the studied isolates, the article shows gradual evolution of the N8 subtype virus.

Genetic polymorphism of the bovine leukemia virus in the Russian federation

2016 ◽  
Vol 42 (6) ◽  
pp. 472-475
Author(s):  
M. I. Gulyukin ◽  
N. G. Kozyreva ◽  
L. A. Ivanova ◽  
T. V. Stepanova ◽  
I. A. Gulyukina
Keyword(s):  
Genetic Polymorphism ◽  
Russian Federation ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
The Russian Federation

scholarly journals Genetic identification of bovine leukaemia virus

2018 ◽  
Vol 6 (2) ◽  
pp. 314-324 ◽  
Author(s):  
Irina Donnik ◽  
Irina Donnik ◽  
Ramil Vafin ◽  
Ramil Vafin ◽  
Aram Galstyan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Research Methods ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Genetic Identification ◽  
Gene Fragment ◽  
Phylogenetic Classification ◽  
Pcr Rflp

Molecular genetic research methods make it possible to evaluate the genetic diversity of bovine leukemia virus (BLV) and are the most informative approaches to its genetic identification. Molecular genetic research methods work well for the phylogenetic analysis of sequenced nucleotide DNA sequences of the provirus, as well as for the polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) according to the phylogenetic classification of the pathogen. The purpose of the research was to study the scientific and methodological approaches to the genetic identification of bovine leukemia virus, integrated into the molecular monitoring of infection of cattle with BLV genotypes. The authors used PCR-RFLP-genotyping and comparative phylogenetic analysis of aligned nucleotide sequences of the env gene fragment of the BLV provirus isolates to detect the genotypic affiliation of the cattle from twenty-one livestock farms of the Republic of Tatarstan. As a result, isolates of four out of ten BLV genotypes were found in the Tatarstani cattle, namely genotypes 1, 4, 7, and 8. The research involved a comparative analysis of 505 nucleotide sequences of a fragment of the BLV env gene, including those deposited in GenBank NCBI. The analysis confirms the inconsistency of several earlier PCR-RFLP typing strategies with the current approach in assessing the genotypic diversity by phylogenetic analysis. The improved strategy of PCR-RFLP genotyping of BLV corresponds with its modern phylogenetic classification. The strategy makes it possible to identify all the known genotypes of the viral pathogen. Its validity has been proved by in silico modelling of restrictogrammes and a phylogenetic analysis of the env gene fragment of 57 reference isolates of ten BLV genotypes that generate 57 genotype-associated combinations of diagnostically significant PCR-RFLP profiles.

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