The course of disease in pigs infected with ASF virus isolates, obtained in different regions of the Russian Federation

2019 ◽  
Vol 22 (4) ◽  
pp. 15-19
Author(s):  
M.E. Vlasov ◽  
◽  
A.K. Sibgatullova ◽  
V.M. Balyshev ◽  
◽  
...  
Keyword(s):  
Russian Federation ◽  
Course Of Disease ◽  
The Russian Federation ◽  
Virus Isolates

scholarly journals Comparative molecular and genetic characterization of rabies viruses (Rabies lyssavirus, Lyssavirus, Rhabdoviridae) circulated in the Russian Federation in 1985–2016

2020 ◽  
Vol 65 (1) ◽  
pp. 41-48
Author(s):  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
M. A. Losich ◽  
A. L. Elakov ◽  
A. M. Gulyukin ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Epidemiology ◽  
Rabies Virus ◽  
Russian Federation ◽  
Amino Acid Sequences ◽  
Phylogenetic Study ◽  
Human Populations ◽  
Human Beings ◽  
The Russian Federation ◽  
Virus Isolates

Introduction. Rabies caused by the neurotropic virus of the genus Lyssavirus, Rhabdoviridae family, which infects all warm-blooded vertebrates including human beings. The homology level of the amino acid sequences for Lyssaviruses nucleoprotein reaches 78–93%.Aim – study the genetic diversity and molecular epidemiology of Lyssaviruses circulated in the Russian Federation in 1985–2016.Material and methods. 54 isolates of rabies virus isolated from animals, and 2 isolates from humans, 4 vaccine strains of rabies virus: RV-97, ERA, Shchelkovo 51, ERAG333 used in phylogenetic study. Phylogenetic analysis was performed using Genbank data on genome fragments of 73 rabies virus isolates and 9 EBLV-1 isolates. DNASTAR V.3.12, Bio Edit 7.0.4.1 and MEGA v.10.0.5, Primer Premier 5 programs have been used.Results. Comparative molecular genetic analysis of genomes fragments of 130 Lissaviruses, isolated on the territory of the RF, Ukraine in 1985-2016, vaccine strains of rabies virus, showed their distribution by geographical feature. Comparison of the nucleoprotein fragments of the rabies virus isolates with vaccine strains revealed 4 marker mutations: V56I (Eurasian group), L/V95W (Central group), D101N/S/T, and N/G106D. Phylogenetic analysis of the isolate «Juli», isolated from a human bitten by a bat proved his belonging to the European Bat lyssavirus-1a.Discussion. Study of the molecular epidemiology of rabies within the Russian Federation allows for the genotyping of the viruses and helps to study the hidden mechanisms of rabies infection in animal and human populations, and to characterize vaccine strains, including during oral vaccination.Conclusion. Further study of the molecular epidemiology of rabies within the Russian Federation and the countries bordering it is important.

scholarly journals Genetic analysis of nucleotide sequences of neuraminidase gene of highly pathogenic avian influenza A/H5N8 virus isolates recovered in the Russian Federation in 2020

2021 ◽  
pp. 301-307
Author(s):  
P. B. Akshalova ◽  
N. G. Zinyakov ◽  
A. V. Andriyasov ◽  
P. D. Zhestkov ◽  
Z. B. Nikonova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Russian Federation ◽  
Highly Pathogenic Avian Influenza ◽  
Nucleotide Sequences ◽  
Highly Pathogenic ◽  
Pathogenic Avian Influenza ◽  
The Russian Federation ◽  
Virus Isolates

Avian influenza is a highly dangerous viral disease that causes huge economic damage to poultry farming. Currently, highly virulent influenza virus with N8 neur- aminidase subtype is quite often detected in populations of domestic and wild birds in various countries of the world. The article provides data on complete nucleotide sequences of the neuraminidase gene of highly pathogenic avian influenza virus isolates recovered in the second half of 2020 from pathological material received from four regions of the Russian Federation. The conducted research showed that the subtype of the isolated virus was N8. According to the phylogenetic analysis, isolates of N8 virus belong to group 8C.4. During the phylogenetic analysis of the neuraminidase, we also took into account data on hemagglutinin classification, according to which H5N8 virus isolates belong to a widespread clade 2.3.4.4. Viruses of the clade were first registered in 2010 in China and they have been circulating up to now. The paper also provides data of a comparative analysis of nucleotide sequences of the studied isolates and the isolates from the international GenBank and GISAID databases, recovered in other countries from 2007 to 2020. During the analysis of the amino acid sequence of the studied isolates, no substitutions were found in the positions that affect resistance to neuraminidase inhibitors. The complete nucleotide sequences of the neuraminidase gene of the avian influenza virus subtype N8 (isolates A/domestic goose/OMSK/1521-1/2020, A/duck/Chelyabinsk/1207-1/2020, A/duck/Saratov/1578-2/2020, A/goose/Tatarstan/1730-2/2020) are published in the international GenBank and GISAID databases. Based on the analysis of the nucleotide sequences of the studied isolates, the article shows gradual evolution of the N8 subtype virus.

scholarly journals A Long-Term Study of the Biological Properties of ASF Virus Isolates Originating from Various Regions of the Russian Federation in 2013–2018

2019 ◽  
Vol 6 (4) ◽  
pp. 99 ◽  
Author(s):  
Andrei Pershin ◽  
Ivan Shevchenko ◽  
Alexey Igolkin ◽  
Ivan Zhukov ◽  
Ali Mazloum ◽  
...  
Keyword(s):  
Russian Federation ◽  
Case Fatality ◽  
African Swine Fever ◽  
Biological Properties ◽  
Long Term Study ◽  
Key Characteristics ◽  
Incubation Periods ◽  
The Russian Federation ◽  
Virus Isolates

Biological properties of the African swine fever (ASF) virus isolates originating from various regions of the Russian Federation (2013–2018) were studied in a series of experimental infections. Comparative analysis allowed us to establish the differences in the key characteristics of the infection, such us the duration of the incubation periods, disease, and the onset of death. The incubation period averaged 4.1 days, varying from 1 to 13 days. An average duration of the disease was 6.3 days and varied from 0 to 18 days. Overall case fatality was 94.5%, and antibodies were detected only in 19.3% of the animals. The biological properties of isolates Odintsovo 02/14 and Lipetsk 12/16 were significantly different from others. For this two, the presence of antibodies to the virus was detected in 71.4% and 75% of animals respectively and mortality levels were of 87.5% and 50%.

Prospects of development of the reference base of the Russian Federation in the field of length measurements

2020 ◽  
pp. 3-5
Author(s):  
Y. G. Zakharenko ◽  
N. A. Kononova ◽  
V. L. Fedorin ◽  
Z. V. Fomkina ◽  
K. V. Chekirda
Keyword(s):  
High Precision ◽  
Russian Federation ◽  
Reference Base ◽  
Length Measurements ◽  
Laser Sources ◽  
The Russian Federation ◽  
Unit Of Length ◽  
Further Development ◽  
532 Nm

The results of the work to create a complex of high-precision hardware for the unit of length reproduction and transferring carried out at “D. I. Mendeleyev Institute for Metrology (VNIIM)” are represented. This complex will serve as the basis for the further development of the reference base of the Russian Federation in the field of length measurements and will allow reproduction of the unit of length at two wavelengths of 633 nm and 532 nm, as well as measurements of the wavelength of laser sources in vacuum in the range from 500 to 1050 nm.

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