Use of electrophoretic spectra of reserve seed proteins to improve typicalness of sunflower parental lines

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

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Heritability and Genetic Advance Estimation From Parental Lines of Hybrid Rice

International Journal of Scientific Research ◽

10.15373/22778179/july2014/5 ◽

2012 ◽

Vol 3 (7) ◽

pp. 11-13

Author(s):

Arpita Shrivastava ◽

◽

D. K. Mishra D. K. Mishra ◽

G. K. Koutu G. K. Koutu ◽

S. K. Singh S. K. Singh

Keyword(s):

Hybrid Rice ◽

Genetic Advance ◽

Parental Lines

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ACTIVITY OF DIGESTIVE ENZYMES IN DUODENAL CHYMUS AND BLOOD IN BROILERS OF PARENTAL LINES AND THE MEAT CROSS DEPENDING ON DIETARY BIOACTIVE ADDITIVES

Sel skokhozyaistvennaya Biologiya ◽

10.15389/agrobiology.2017.6.1226eng ◽

2017 ◽

Vol 52 (6) ◽

pp. 1226-1233

Author(s):

V.I. Fisinin ◽

◽

I.A. Egorov ◽

V.G. Vertiprakhov ◽

A.A. Grozina ◽

...

Keyword(s):

Digestive Enzymes ◽

Parental Lines

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Combining ability and heterosis studies across environments in linseed (Linum usitatissimum L.)

Acta Agronomica Hungarica ◽

10.1556/aagr.59.2011.1.9 ◽

2011 ◽

Vol 59 (1) ◽

pp. 87-102 ◽

Cited By ~ 3

Author(s):

S. Sood ◽

N. Kalia ◽

S. Bhateria

Keyword(s):

Seed Yield ◽

Linum Usitatissimum ◽

Combining Ability ◽

Yield Components ◽

Block Design ◽

Linum Usitatissimum L ◽

Randomized Block Design ◽

Component Traits ◽

Parental Lines ◽

Fibre Yield

Combining ability and heterosis were calculated for fourteen lines of linseed in a line × tester mating design using twelve lines and two diverse testers in two different environments. The hybrids and parental lines were raised in a completely randomized block design with three replications to investigate seed and fibre yield and their component traits. Genetic variation was significant for most of the traits over environments. Combining ability studies revealed that the lines KL-221 and LCK-9826 were good general combiners for seed yield and most of its components, whereas LMH-62 and LC-2323 were good general combiners for yield components only. Moreover, KL-221 was also a good general combiner for fibre yield. Similarly, B-509 and Ariane were good general combiners for fibre yield and most of its components. Among the specific cross combinations, B-509 × Flak-1 was outstanding for seed yield per plant and B-509 × KL-187 and LC-2323 × LCK-9826 for fibre yield per plant, with high SCA effects. In general, the hybrids excelled their respective parents and the standard checks for most of the characters studied. Based on the comparison of mean performance, SCA effects and the extent of heterosis, the hybrids LC-2323 × LCK-9826 and B-509 × KL-221 appeared to be the most promising for both seed and fibre yield. Other promising combinations were LC-2323 × KL-210 and B-509 × Ariane for seed and fibre yield, respectively. The superiority of LC-2323, LCK-9826, KL-221, B-509 and Ariane as good general combiners was further confirmed by the involvement of these parents in the desirable cross combinations.

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Study on floral traits and seed setting in parental lines of hybrid rice during early ahu and kharif seasons

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.78.3.2 ◽

2018 ◽

Vol 78 (3) ◽

Author(s):

Aradhana Phukan ◽

P. K. Barua ◽

D. Sarma ◽

S. D. Deka

Keyword(s):

Seed Set ◽

Flag Leaf ◽

Pollen Dispersal ◽

Pollen Sterility ◽

Leaf Length ◽

Floral Traits ◽

Opening Angle ◽

Leaf Angle ◽

Seed Setting ◽

Parental Lines

Two CMS lines, IR 58025A and IR 68888A along with their maintainers and two fertility restorers, LuitR and IR 36R, were evaluated for flower and plant characters during early ahu (February-June) and kharif (July-November) seasons. IR 58025A showed longer stigmata and styles, and higher spikelet Length/Breadth (L/B) ratio while IR 68888A showed broader stigmata and wider glume opening angle in both the seasons. IR 68888A also exhibited higher pollen sterility during early ahu. IR 36R was characterized with broad anthers. LuitR showed longer and broader anthers with more pollen than others. Plant height, flag leaf length, flag leaf width and area were higher in IR 36R. Panicle exsertion was complete in pollen parents whereas it was 78-80% in CMS lines. The widest flag leaf angle was found in IR 58025B during early Ahu and in IR 36R during kharif. Kharif season was more favourable for growth of the plants with higher seed set percentage while floral traits of the CMS lines were better expressed in early Ahu. Manipulation of the seeding sequence of the parental lines in early Ahu is warranted for better seed set provided the seed crop escapes heavy premonsoon showers during reproductive stage. IR 68888A/LuitR was a good combination for pollen dispersal and seed setting.

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