GENETIC DIVERSITY IN BAMBARA GROUNDNUT (Vigna subterranean) AS REVEALED BY MOLECULAR WEIGHTS OF THE SEEDS’ PROTEINS

2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Proteins ◽  
Electrophoretic Analysis ◽  
Protein Characterization ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Phylogenetic Relatedness ◽  
Molecular Weights ◽  
National Centre ◽  
Standard Marker

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

Genetic diversity in Bambara groundnut (Vigna subterranea (L.) Verdc.) as revealed by phenotypic descriptors and DArT marker analysis

2011 ◽  
Vol 59 (3) ◽  
pp. 347-358 ◽  
Author(s):  
Bode A. Olukolu ◽  
Sean Mayes ◽  
Florian Stadler ◽  
N. Quat Ng ◽  
Iyiwole Fawole ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dart Marker ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Marker Analysis

Genetic diversity in bambara groundnut (Vigna subterranea (L.) Verdc) landraces revealed by AFLP markers

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1175-1180 ◽  
Author(s):  
F J Massawe ◽  
M Dickinson ◽  
J A Roberts ◽  
S N Azam-Ali
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
Aflp Markers ◽  
Group Method ◽  
Aflp Analysis ◽  
Vigna Subterranea ◽  
Marker Assisted Breeding ◽  
Pair Group

Bambara groundnut (Vigna subterranea (L.) Verdc), an African indigenous legume, is popular in most parts of Africa. The present study was undertaken to establish genetic relationships among 16 cultivated bambara groundnut landraces using fluorescence-based amplified fragment length polymorphism (AFLP) markers. Seven selective primer combinations generated 504 amplification products, ranging from 50 to 400 bp. Several landrace-specific products were identified that could be effectively used to produce landrace-specific markers for identification purposes. On average, each primer combination generated 72 amplified products that were detectable by an ABI Prism 310 DNA sequencer. The polymorphisms obtained ranged from 68.0 to 98.0%, with an average of 84.0%. The primer pairs M-ACA + P-GCC and M-ACA + P-GGA produced more polymorphic fragments than any other primer pairs and were better at differentiating landraces. The dendrogram generated by the UPGMA (unweighted pair-group method with arithmetic averaging) grouped 16 landraces into 3 clusters, mainly according to their place of collection or geographic origin. DipC1995 and Malawi5 were the most genetically related landraces. AFLP analysis provided sufficient polymorphism to determine the amount of genetic diversity and to establish genetic relationships in bambara groundnut landraces. The results will help in the formulation of marker-assisted breeding in bambara groundnut.Key words: under-utilized, African legume, molecular markers.

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H.I. Amadou ◽  
P.J. Bebeli ◽  
P.J. Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Bambara Groundnut ◽  
Vigna Subterranea

scholarly journals DNA fingerprinting, fixation-index (Fst), and admixture mapping of selected Bambara groundnut (Vigna subterranea [L.] Verdc.) accessions using ISSR markers system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Md Mahmudul Hasan Khan ◽  
Mohd Y. Rafii ◽  
Shairul Izan Ramlee ◽  
Mashitah Jusoh ◽  
Md Al Mamun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Environmental Sustainability ◽  
Crop Improvement ◽  
Principal Component ◽  
Issr Markers ◽  
Bambara Groundnut ◽  
Fixation Index ◽  
Vigna Subterranea ◽  
Information Index ◽  
Issr Primers

AbstractAs a new crop in Malaysia, forty-four Bambara groundnut (Vigna subterranea L. verdc.) genotypes were sampled from eleven distinct populations of different origins to explore the genetic structure, genetic inconsistency, and fixation index. The Bambara groundnut, an African underutilized legume, has the capacity to boost food and nutrition security while simultaneously addressing environmental sustainability, food availability, and economic inequalities. A set of 32 ISSRs were screened out of 96 primers based on very sharp, clear, and reproducible bands which detected a total of 510 loci with an average of 97.64% polymorphism. The average calculated value of PIC = 0.243, RP = 5.30, H = 0.285, and MI = 0.675 representing the efficiency of primer set for genetic differentiation among the genotypes. The ISSR primers revealed the number of alleles (Na = 1.97), the effective number of alleles (Ne = 1.38), Nei's genetic diversity (h = 0.248), and a moderate level of gene flow (Nm = 2.26) across the genotypes studied. The estimated Shannon’s information index (I = 0.395) indicates a high level of genetic variation exists among the accessions. Based on Nei’s genetic dissimilarity a UPMGA phylogenetic tree was constructed and grouped the entire genotypes into 3 major clusters and 6 subclusters. PCA analysis revealed that first principal component extracted maximum variation (PC1 = 13.92%) than second principal component (PC2 = 12.59%). Bayesian model-based STRUCTURE analysis assembled the genotypes into 3 (best ΔK = 3) genetic groups. The fixation-index (Fst) analysis narrated a very great genetic diversity (Fst = 0.19 to 0.40) exists within the accessions of these 3 clusters. This investigation specifies the effectiveness of the ISSR primers system for the molecular portrayal of V. subterranea genotypes that could be used for genetic diversity valuation, detection, and tagging of potential genotypes with quick, precise, and authentic measures for this crop improvement through effective breeding schemes.

scholarly journals Analisis Kesesuaian Marka SSR ( Simple Sequence Repeats ) untuk Identifikasi Keragaman Genetik pada Kacang Bambara Asal Jawa Barat

Agrikultura ◽  
2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Safarinda Nurdianawati ◽  
Noladhi Wicaksana ◽  
Anas Anas
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Polymorphic Information Content ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Suitability Analysis ◽  
West Java ◽  
Crop Development ◽  
Alternative Sources ◽  
Simple Sequence

ABSTRACT Suitability Analysis of SSR Marker ( Simple Sequence Repeats ) for Genetic Diversity Identification in Bambara Groundnut West Java Origin Bambara groundnut ( Vigna subterranea ) is one of legume crops that has not been widely cultivated. It becomes food alternative sources in Indonesia as it has high of protein and carbohydrate content and low fat. Furthermore, bambara groundnut should be good production in marginal land. Molecular marker has been used for any crop development such as genetic diversity, matting system, clones identification, etc. This research used neutral genetic markers (SSR) to study genetic diversity in Bambara groundut population from West Java. Fifteen primers has used and the results showed that primer 1 and primer 19 were polymorphic and informative because the locus has high HE (heterozygosity) and PIC (Polymorphic Information Content) value compare to others. The higher HE and PIC value the more informative to distinguish each individual between genotype in one population. Keywords: Bambara groundnut ( Vigna subterranea ), Genetic diversity, Simple sequence repeats (SSR)  ABSTRAK Kacang bambara ( Vigna subterranea ) merupakan salah satu tanaman legume yang belum terlalu banyak dibudidayakan dan dapat menjadi sumber pangan alternative di Indonesia karena kandungan protein dan karbohidratnya  cukup tinggi dan rendah lemak. Selain itu, tanaman ini juga dilaporkan dapat berproduksi dengan baik pada lahan-lahan marginal. Penggunaan marka molekuler telah banyak digunakan pada tanaman untuk berbagai tujuan antara lain untuk mengevaluasi keragaman genetik suatu populasi, menduga system perkawinan, identifikasi klon dan lain-lain. Dalam penelitian ini digunakan marka netral (SSR) untuk mempelajari keragaman genetik populasi Kacang bambara ( Vigna subterranea ) asal Jawa Barat. Dari lima belas primer, primer 1 dan primer 19 merupakan primer yang bersifat polimorfik dan informative karena lokus tersebut memiliki nilai HE (heterozigositas) dan PIC ( P o l y m o r p h i c I n f o r m a t i o n Con tent ) yang tinggi dibandingkan dengan primer-primer lainnya. Semakin tinggi nilai HE dan PIC yang dihasilkan maka primer tersebut semakin informatif dalam membedakan individu antar genotip dalam populasi. Kata Kunci : Kacang bambara ( Vigna subterranea ), Keragaman genetik, Simple Sequence Repeats (SSR)

scholarly journals Genetic diversity of Bambara groundnut (Vigna subterranea (L.) verdc.) landraces in Kenya using microsatellite markers

2015 ◽  
Vol 14 (4) ◽  
pp. 283-291 ◽  
Author(s):  
O. Odongo Forrester ◽  
E.Oyoo Maurice ◽  
Wasike Victor ◽  
O. Owuoche James ◽  
Karanja Laura ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Bambara Groundnut ◽  
Vigna Subterranea

scholarly journals Assessment of genetic diversity and structure of Bambara groundnut [Vigna subterranea (L.) verdc.] landraces in South Africa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adré Minnaar-Ontong ◽  
Abe S. Gerrano ◽  
Maryke T. Labuschagne
Keyword(s):  
South Africa ◽  
Genetic Diversity ◽  
Ssr Markers ◽  
Southern Africa ◽  
Crop Improvement ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Legume Crop ◽  
Population Structure Analysis ◽  
Genetic Diversity And Structure

AbstractWith its drought tolerant and protein-rich properties, Bambara groundnut [Vigna subterranea (L.) Verdc.], an indigenous African legume crop can contribute immensely to food security. This miracle crop is used as food and for the enhancement of soil fertility in South Africa. Knowledge on the genetic diversity and structure among the Bambara groundnut landraces can pave the way for the effective use and cultivation of this crop in southern Africa, especially South Africa. The aim of this study was to assess the genetic diversity and structure among Bambara groundnut landraces collected across South Africa and compared to a limited number of accessions from southern Africa using SSR markers. Seventy-eight Bambara groundnut accessions were genotyped using 19 Bambara specific SSR markers. SSR loci explored in this study, were all polymorphic. A total of 127 alleles were detected with a mean of 6.7 alleles per locus. Allele diversity and frequency among genotypes varied from 0.21 to 0.85 with an average of 0.62 per locus. Genetic variation as described by the analysis of molecular variance indicated higher genetic diversity (92%) within landraces than between (8%) different landraces. Population structure analysis showed that three subpopulations existed, and most of the South African accessions were restricted to one subpopulation, indicating that Bambara landraces has the ability to form unique haplotypes in different environments. Information harnessed in this study is helpful for further use in breeding programs for crop improvement.

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