THE BACTERICIDAL PROPERTIES OF CHITOSAN DERIVATIVES TO PROTECT IMPLANTS

2019 ◽  
Vol 0 (2) ◽  
pp. 21-25
Author(s):  
M.A. Guseinova ◽  
◽  
E.V. Salomatina ◽  
L.A. Smirnova ◽  
O.N. Smirnova ◽  
...  
Author(s):  
Serhii Zinoviev ◽  
Andrii Kurman ◽  
Dmytro Bindiug ◽  
Pavlo Grubich ◽  
Liubov Lepeta

The introduction of the newest technologies of organic pig breeding has the basic principle of minimizing the use in pigs of chemicals, trace amounts of which in pig production adversely affect the quality of products and the health of consumers. The transition to herbal remedies, the maximum possible rejection of chemical synthesis products, is inhibited, in the main, by the relatively small spectrum of plant compounds with the investigated activity. According to the results of previous studies, six working solutions of Juglans regia L phytosupply extracts - potential phytosine disinfectants were developed: To investigate the bactericidal activity of the experimental preparations of Juglans regia, flushes were made from the internal structures and technological equipment of the housing of the pig stock of the experimental base v. Takhtaulove. Obtained samples of typical banal microflora of the pig complex were mixed to obtain a bacterial-containing suspension averaged over the concentration of microbial bodies and the spectrum of types of microflora in the microbiota. When cultured on nutrient media, with the superficial sowing of a suspension of banal microflora of a pig in the presence of a potential phytodisinfectant, processes of development of individual colonies of the microflora are observed. The amount and intensity of their development, the degree of inhibition of life, assess the level of bactericidal activity of the extracts. The highest bactericidal and fungicidal activity, and consequently, the potential efficacy as a possible disinfectant in the technology of organic pig breeding was revealed by the extracts of phytosupply Juglans regia L No. 2 (extractant - acetic acid to 5 %, nut of wax ripeness) and 4 (extractant, alcohol 20 % nutrient wax ripeness (proven after obtaining the primary extract of up to 5 % alcohol), which have bactericidal properties against gram-positive cocci and gram-negative sticks, but have a weak effect on the spore-forming bacilli. Key words: organic pig breeding, bactericidal activity, phyto-disinfectant, commonplace typical microflora of a pig complex, phyto row materials, walnut extract.


2018 ◽  
Author(s):  
E. E. Said-Galiev ◽  
M. S. Rubina ◽  
A. Yu. Vasil’kov ◽  
A. V. Naumkin ◽  
A. R. Khokhlov ◽  
...  

2021 ◽  
Vol 263 ◽  
pp. 124432
Author(s):  
Vahid Zarghami ◽  
Mohammad Ghorbani ◽  
Kamran Pooshang Bagheri ◽  
Mohammad Ali Shokrgozar

2021 ◽  
Vol 22 (5) ◽  
pp. 2511
Author(s):  
Anna Maria Piras ◽  
Semih Esin ◽  
Arianna Benedetti ◽  
Giuseppantonio Maisetta ◽  
Angela Fabiano ◽  
...  

The authors wish to make the following corrections to this paper [...]


2021 ◽  
Vol 105 (3) ◽  
pp. 1215-1226
Author(s):  
Wojciech Makowski ◽  
Aleksandra Królicka ◽  
Anna Nowicka ◽  
Jana Zwyrtková ◽  
Barbara Tokarz ◽  
...  

Abstract The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg × g−1 DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria. Key points • Rhizobium-mediated transformation created Dionaea muscipula teratomas. • Transformed plants had highly increased synthesis of phenolic compounds. • The MBC value was connected with plumbagin and phenolic acid concentrations.


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