scholarly journals Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

2021 ◽  
Vol 105 (3) ◽  
pp. 1215-1226
Author(s):  
Wojciech Makowski ◽  
Aleksandra Królicka ◽  
Anna Nowicka ◽  
Jana Zwyrtková ◽  
Barbara Tokarz ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Antibacterial Properties ◽  
Biologically Active ◽  
Plant Genome ◽  
Resistant Bacteria ◽  
Shoot Cultures ◽  
Transformed Plants ◽  
Dionaea Muscipula ◽  
Bactericidal Properties

Abstract The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg × g−1 DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria. Key points • Rhizobium-mediated transformation created Dionaea muscipula teratomas. • Transformed plants had highly increased synthesis of phenolic compounds. • The MBC value was connected with plumbagin and phenolic acid concentrations.

scholarly journals Assessment of the potential of Artemisia annua L. as a source of biologically active substances in Belarus

2019 ◽  
Vol 24 ◽  
pp. 290-295
Author(s):  
H. G. Shutava ◽  
S. N. Shysh ◽  
P. S. Shabunya ◽  
S. A. Fatykhava ◽  
E. D. Skakovski ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Essential Oils ◽  
Phenolic Acids ◽  
Artemisia Annua ◽  
Hydroxycinnamic Acids ◽  
Biologically Active ◽  
Artemisia Annua L ◽  
Agroclimatic Zone ◽  
Ground Mass

Aim. The aim of the work was to assess the composition of biologically active compounds in plants of Artemisia annua L. Methods. The plants were grown on the experimental plot of the Central Botanical Garden of the National Academy of Sciences of Belarus. Essential oil from the above-ground mass was isolated by the method of water-steam distillation. To analyze the content of flavonoids and hydroxycinnamic acids, we used the method of quantitative extraction and spectrophotometric determination of the total content of hydroxycinnamic acids in the presence of flavonoids in plant extractive substances, and the method of Folin-Chocalteu was used to determine the content of phenolic compounds. Analysis of the essential oils was performed by GC and NMR. The analysis of the content of phenolic acids in extracts was performed by HPLC. Results. The genetic potential of A. annua was evaluated under conditions of the central agroclimatic zone of Belarus. The yield and composition of essential oils, the content of phenolic compounds and flavonoids, the composition of phenolic acids were studied. Conclusions. A. annua in the conditions of the central agroclimatic zone of Belarus contains essential oil, which is dominated by isoartemisia ketone, β-selinene, β-myrcene and camphor. In the above-ground mass phenolic acids including chlorogenic acid and isomers of caffeoylquinic acid were predominant in the composition of phenolic compounds. Keywords: annual wormwood, essential oil, phenolic compounds, phenolic acids.

Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp.

10.5219/1360 ◽  
2020 ◽  
Vol 14 ◽  
pp. 393-401
Author(s):  
Yulia Vinogradova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Eva Ivanišová ◽  
Ján Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Biologically Active ◽  
Polyphenol Compounds ◽  
Dpph Method ◽  
Ethanol Extracts

Chokeberry (Aronia Medik.) is a non-traditional fruit plant known as a rich source of biologically active compounds and inhibits the numerous biological activities. We compared the antioxidant activity and phenolic compounds of fruits between widely cultivated Aronia mitschurinii (AM-TCH, from Tchekhov district; AM-D, from Dmitrov district; AM-OZ, from Orekhovo-Zuevsky district of Moscow region, Russia) and introduced North American Aronia species (Aronia arbutifolia (AA-M), A. melanocarpa (AML-M), A. × prunifolia (AP-M), which have not been planted yet in the arboretum of Main Botanical Garden of the Russian Academy of Sciences (Moscow). Studying samples were collected in their secondary distribution range. Ethanolic extracts were determined for antioxidant capacity (antioxidant activity by DPPH and phosphomolybdenum methods, the total content of polyphenols, flavonoids, phenolic acids) and measured spectrophotometrically. As standards were used Trolox (TE) for antioxidant activities, gallic acid (GAE) for polyphenol content, quercetin (QE) for flavonoid content, caffeic acid (CAE) for phenolic acid content. The antioxidant activity by DPPH method in ethanol extracts of investigated plants was from 6.96 (AM-D) to 8.89 (AM-OZ) mg TE.g-1 DW. Reducing the power of investigated extracts exhibited activity from 151.47 (AM-OZ) to 297.8 (AA-M) mg TE.g-1 DW. The content of polyphenol compounds determined from 25.98 (AM-TCH) to 54.39 (AA-M) mg GAE.g-1 DW, phenolic acids content was from 7.76 (AP-M) to 11.87 (AM-D) mg CAE.g-1 DW and the content of flavonoids detected from 8.12 (AM-OZ) to 16.62 (AM-D) mg QE.-1 DW. Obtained data showed a strong correlation between the content of polyphenol compounds and reducing the power of extracts (r = 0.700), between flavonoids and phenolic acids (r = 0.771) and also between phenolic acids and reducing power (r = 0.753) in Aronia ethanol extracts. Fruits of investigated species of Aronia can be propagated as a source of polyphenol compounds with antioxidant activity and obtained results may use for farther pharmacological study.

scholarly journals Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1794 ◽  
Author(s):  
Wojciech Makowski ◽  
Krzysztof Michał Tokarz ◽  
Barbara Tokarz ◽  
Rafał Banasiuk ◽  
Karolina Witek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Total Phenolic ◽  
Tissue Cultures ◽  
Venus Flytrap ◽  
Dionaea Muscipula ◽  
Plant Culture

The carnivorous plant Dionaea muscipula J. Ellis (Venus flytrap) is a widely known medical herb, capable of producing various phenolic compounds known for their strong antioxidant and antibacterial properties. In the pharmaceutical industry, Venus flytrap is grown in tissue cultures, as the natural population of D. muscipula is very limited. Here, we describe an improved method to increase the quantity and quality of phenolic compounds produced in D. muscipula. This is achieved by combining biotic elicitation (using Cronobacter sakazakii bacteria lysate) of D. muscipula cultured with rotary shaking (hydromechanical stress), which we describe here for the first time. The antibacterial activity and the antioxidant properties of the obtained compounds were studied on two antibiotic-resistant human pathogenic bacteria. The proposed plant culture conditions resulted in an increase in fresh weight, as well as a higher total phenolic content, in comparison to traditional tissue cultures on agar-solidified medium. With the use of high-performance liquid chromatography, we demonstrated that the described elicitation strategy leads to an increased synthesis of myricetin, caffeic acid, ellagic acid and plumbagin in D. muscipula tissue. We also found that a higher level of antioxidant activity, exhibited by the plant extract, corresponded with its higher phenylpropanoid content. The bactericidal activity of the extract against Staphylococcus aureus was dependent on the duration of plant culture under described elicitation conditions, whereas neither elicitation condition (duration or elicitor concentration) seemed relevant for the bactericidal activity of the extract towards Escherichia coli. This suggest that Gram-negative bacteria are less sensitive to compounds derived from Venus flytrap tissue.

Quantitative analysis of polyphenols and antioxidant activity in four Daphne L. species

2012 ◽  
Vol 7 (6) ◽  
pp. 1092-1100 ◽  
Author(s):  
Renata Grubešić ◽  
Dario Kremer ◽  
Marijana Končić ◽  
Jadranka Rodríguez ◽  
Marko Randić
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Total Polyphenols ◽  
The Family

AbstractThe content of biologically active phenolic compounds (total polyphenols, tannins, flavonoids, and phenolic acids) were determined using spectrophotometry in four wild Croatian species of Daphne L. in the family Thymelaeaceae (Daphne alpina, D. cneorum, D. laureola, and D. mezereum). The concentration of total flavonoids (TF) was highest in the leaves of these Daphne species (0.12–0.51% dry herb weight, DW) whereas the content of other phenolic compounds analyzed were highest in the roots, including total polyphenols (TP; 2.71–19.03% DW), tannins (T; 1.14–7.39% DW), and total phenolic acids (TPA; 0.12–0.87% DW). D. alpina contained the highest amount of polyphenols, with the exception of flavonoids, where maximum concentrations were found in D. laureola. We also examined the antioxidant activity of leaf, stem, and root extracts. All extracts analyzed demonstrated high free radical scavenging activity with the highest concentration in the leaf extracts of D. alpina. Leaf extracts of D. cneorum showed the highest antioxidant activity in a β-carotene bleaching assay.

Accumulation of biomass and phenolic compounds in Polish and Mongolian great burnet (Sanguisorba officinalis L.) populations

2014 ◽  
Vol 60 (3) ◽  
pp. 44-55 ◽  
Author(s):  
Katarzyna Bączek
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Raw Materials ◽  
Epigallocatechin Gallate ◽  
Intraspecific Variability ◽  
Total Content ◽  
Biologically Active ◽  
Ex Situ ◽  
Epicatechin Gallate ◽  
Sanguisorba Officinalis

Summary Eight Polish and six Mongolian great burnet populations were evaluated ex situ in respect of the mass of underground organs and accumulation of biologically active compounds. In the raw materials collected in autumn of the second year of plant vegetation, total content of tannins and phenolic acids as well as the content of phenolic compounds identified by HPLC were determined. Distinct differences between Polish and Mongolian populations and their high intraspecific variability, concerning analysed parameters, was observed. The mass of underground organs of Polish populations was higher than in Mongolian ones (595.0 and 523.5 g × plant-1, respectively, for fresh mass). Polish populations were characterised by significantly higher total content of tannins and phenolic acids (6.02 and 1.60%, respectively) in comparison with Mongolian ones (2.89 and 0.97%, respectively). In the investigated raw materials eight phenolic compounds were identified, namely: /-/-epigallocatechin, /+/-catechin, /-/-epicatechin, /-/-epicatechin gallate, /-/-epigallocatechin gallate, astragalin, ellagic and gallic acids. In all populations, the dominating compound, was /-/-epigallocatechin. The contents of /-/-epigallocatechin, /-/-epicatechin gallate and gallic acid were distinctly higher in Polish populations.

scholarly journals Innovative Method for Obtaining Biologically Active Compounds from Brewery Mash

2021 ◽  
Vol 51 (1) ◽  
pp. 113-124
Author(s):  
Konstantin Kobelev ◽  
Marina Gernet ◽  
Irina Gribkova
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Raw Materials ◽  
Ethanol Solution ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Cellulolytic Enzyme ◽  
Enzyme Preparations ◽  
Naoh Solution ◽  
Active Substances

Introduction. Brewery mash, or brewer’s spent grain (BSG), is a by-product of brewing industry. It is known to contain valuable biologically active substances. However, their extraction is complicated by the presence of various polymers. The research featured various physicochemical methods for obtaining valuable biological compounds from brewery waste. The new method modified complex non-starch polysaccharides, lignin, arabinoxylans, and other high-molecular compounds associated with phenolic compounds. The research objective was to solve the problem of recycling industrial by-products that accumulate in large quantities and require expensive processing or disposal. The paper introduces new technological approaches for deep processing of BSG as a source of secondary raw materials in order to obtain extracts fortified with polyphenolic compounds. Study objects and methods. The research featured BSG from malt subjected to treatment with ECA-activated water (catholyte with pH 9.6 ± 0.1), followed by enzymatic hydrolysis of cellulolytic enzyme preparations and extraction with a polar solvent of the resulting free polyphenolic substances. The experiments were based on standard methods for assessing the content of various biologically active substances. Results and discussion. A 70% water-ethanol solution proved to be optimal at the BSG:extractant ratio of 2:1, process temperature = 50 ± 2°C, and extraction time = 60 ± 5 min. Under the same conditions, 70 %vol. of beer distillate made it possible to extract phenolic acids, flavonoid rutin, irreplaceable and nonessential amino acids, and non-starch polysaccharide β-glucan from the BSG matrix. The BSG treatment with 1M NaOH solution delivered viscous hydrolysates fortified with flavonoids rutin and quercetin, which did not happen when acid hydrolysis was used. The combined use of ECA-treated water (catholyte with pH 9.6 ± 0.1) for 24 ± 0.05 h, combined with biocatalysis with the enzyme preparation Viskoflo MG for 2 ± 0.05 h, made it possible to obtain BSG extracts with a high content of phenolic acids and aldehydes, as well as flavonoid rutin. Conclusion. The study revealed the mechanism of hydrolytic decomposition of BSG non-starch polysaccharides, considering the compounds contained in the extracts. The BSG hydrolysates fortified with various phenolic compounds can be used in various food technologies, e.g., in fermented drinks.

Silver in biology and medicine: Opportunities for metallomics researchers

Metallomics ◽  
2020 ◽  
Author(s):  
Harley D Betts ◽  
Carole Whitehead ◽  
Hugh H Harris
Keyword(s):  
Antibacterial Properties ◽  
Pseudomonas Stutzeri ◽  
The Body ◽  
Blue Staining ◽  
Resistant Bacteria ◽  
Oxygen Species ◽  
Antibiotic Resistant ◽  
Bactericidal Properties ◽  
Soft Metal ◽  
The Brain

Abstract The antibacterial properties of silver have been known for centuries and the threat of antibiotic resistant bacteria has led to renewed focus on the noble metal. Silver is now commonly included in a range of household and medical items to imbue them with bactericidal properties. Despite this, the chemical fate of the metal in biological systems is poorly understood. Silver(I) is a soft metal with high affinity for soft donor atoms (S and N), and displays much similarity to the chemistry of Cu(I). In bacteria, interaction of silver with the cell wall/membrane, DNA, and proteins and enzymes can lead to cell death. Additionally, the intracellular generation of reactive oxygen species by silver is posited to be a significant antimicrobial action. While the antibacterial action of silver is well known, bacteria found in silver mines (Pseudomonas stutzeri) display resistance against it through use of a protein ensemble throught to have been specifically developed for the metal, highlighting the need for judicious use. In mammals, approximately 10–20% of ingested silver is retained by the body and thought to predominantly localise in the liver or kidneys. Chronic exposure can result in argyria, a condition characterised by blue staining of the skin, resulting from subdermal deposition of silver (as Ag(0) and sulfides). However, more insidious side effects, such as inclusions in the brain, seizures, liver/kidney damage, and immunosuppression have also been reported. Here we hope to highlight the current understanding of the biological chemistry of silver and the necessity for continued study of these systems to fill existing gaps in knowledge.

scholarly journals A Critical Review of Phenolic Compounds Extracted from the Bark of Woody Vascular Plants and Their Potential Biological Activity

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1182 ◽  
Author(s):  
Corneliu Tanase ◽  
Sanda Coșarcă ◽  
Daniela-Lucia Muntean
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Vascular Plants ◽  
Fruits And Vegetables ◽  
Biological Effects ◽  
Vascular Plant ◽  
Antibacterial Properties ◽  
Extraction Methods ◽  
Biologically Active ◽  
Future Research

Polyphenols are one of the largest and most widespread groups of secondary metabolites in the plants world. These compounds are of particular interest due to their occurrence and the properties they possess. The main sources of phenolic compounds are fruits and vegetables, but lately, more and more studies refer to woody vascular plants, especially to bark, as an important source of phenolic compounds with a potential biological effect. This study aims to bring together information on the phenolic compounds present in the bark of woody vascular plants by discussing extraction methods, the chemical composition of the extracts and potential biological effects. The literature data used in this paper were collected via PubMed (2004–2019). Search terms were: bark, rhytidome, woody vascular plant, polyphenols, phenolic compounds, biologic activity, antioxidant, immunostimulatory, antimutagenic, antibacterial, anti-inflammatory, and antitumoral. This paper intends to highlight the fact that the polyphenolic extracts obtained from the bark of woody vascular plants represent sources of bioactive compounds with antioxidant, immunostimulatory, antimutagenic, antibacterial properties, etc. Future research directions should be directed towards identification and isolation of bioactive compounds. Consequently, biologically active compounds obtained from the bark of woody plants could be exploited on an industrial scale.

scholarly journals Effect of Elicitation with (+)-Usnic Acid on Accumulation of Phenolic Acids and Flavonoids in Agitated Microshoots of Eryngium alpinum L.

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5532
Author(s):  
Małgorzata Kikowska ◽  
Barbara Thiem ◽  
Karolina Jafernik ◽  
Marta Klimek-Szczykutowicz ◽  
Elżbieta Studzińska-Sroka ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Usnic Acid ◽  
Shoot Cultures ◽  
Biomass Growth ◽  
Effective Technique ◽  
In Vitro Shoot Cultures

The present work was aimed at studying the potential of elicitation on the accumulation of phenolic compounds in in vitro shoot cultures of Eryngium alpinum L., a protected plant from the Apiaceae family. The study examined the influence of (+)-usnic acid on the biomass growth as well as on the biosynthesis of the desired flavonoids and phenolic acids in the cultured microshoots. The phenolic compound content was determined by HPLC-DAD. The flavonoid of the highest concentration was isoquercetin, and the phenolic acids of the highest amount were rosmarinic acid, caffeic acid and 3,4-dihydroxyphenylacetic acid, both in the non-elicited and elicited biomass. Isoquercetin accumulation was efficiently increased by a longer elicitation with a lower concentration of lichenic compound (107.17 ± 4.67 mg/100 g DW) or a shorter elicitation with a higher concentration of acid (127.54 ± 11.34 and 108.37 ± 12.1 mg/100 g DW). Rosmarinic acid production generally remained high in all elicited and non-elicited microshoots. The highest content of this acid was recorded at 24 h of elicitation with 3.125 µM usnic acid (512.69 ± 4.89 mg/100 g DW). The process of elicitation with (+)-usnic acid, a well-known lichenic compound with allelopathic nature, may therefore be an effective technique of enhancing phenolic compound accumulation in alpine eryngo microshoot biomass.

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