Histological Structure of Vocal Fold Lamina Propria in Foetal Larynx

1984 ◽  
Vol 97 (5-6) ◽  
pp. 403-406 ◽  
Author(s):  
Radovan Suboti ◽  
Santa Večerina ◽  
Zvonimir Krajina ◽  
Minoru Hirano ◽  
Shigejiro Kurita
Keyword(s):  
Lamina Propria ◽  
Vocal Fold ◽  
Histological Structure

Reinke's Edema: Phonatory Mechanisms and Management Strategies

1997 ◽  
Vol 106 (7) ◽  
pp. 533-543 ◽  
Author(s):  
Steven M. Zeitels ◽  
Glenn W. Bunting ◽  
Robert E. Hillman ◽  
Traci Vaughn
Keyword(s):  
Lamina Propria ◽  
Fundamental Frequency ◽  
Vocal Fold ◽  
Management Strategies ◽  
Vocal Folds ◽  
Vocal Fold Vibration ◽  
Reinke’S Edema ◽  
Subglottal Pressure ◽  
Almost All ◽  
Superficial Lamina

Reinke's edema (RE) has been associated typically with smoking and sometimes with vocal abuse, but aspects of the pathophysiology of RE remain unclear. To gain new insights into phonatory mechanisms associated with RE pathophysiology, weused an integrated battery of objective vocal function tests to analyze 20 patients (19 women) who underwent phonomicrosurgical resection. Preoperative stroboscopic examinations demonstrated that the superficial lamina propria is distended primarily on the superior vocal fold surface. Acoustically, these individuals have an abnormally low average speaking fundamental frequency (123 Hz), and they generate abnormally high average subglottal pressures (9.7 cm H20). The presence of elevated aerodynamic driving pressures reflects difficulties in producing vocal fold vibration that are most likely the result of mass loading associated with RE, and possibly vocal hyperfunction. Furthermore, it is hypothesized that in the environment of chronic glottal mucositis secondary to smoking and reflux, the cephalad force on the vocal folds by the subglottal driving pressure contributes to the superior distention of the superficial lamina propria. Surgical reduction of the volume of the superficial lamina propria resulted in a significant elevation in fundamental frequency (154 Hz) and improvement in perturbation measures. In almost all instances, both the clinician and the patient perceived the voice as improved. However, these patients continued to generate elevated subglottal pressure (probably a sign of persistent hyperfunction) that was accompanied by visually observed supraglottal strain despite the normalsized vocal folds. This finding suggests that persistent hyperfunctional vocal behaviors may contribute to postsurgical RE recurrence if therapeutic strategies are not instituted to modify such behavior.

Immunocytochemical Study of Proteoglycans in Vocal Folds

1996 ◽  
Vol 105 (1) ◽  
pp. 6-11 ◽  
Author(s):  
Agnieszka S. Pawlak ◽  
Elizabeth Hammond ◽  
Thomas Hammond ◽  
Steven D. Gray
Keyword(s):  
Chondroitin Sulfate ◽  
Lamina Propria ◽  
Vocal Fold ◽  
Keratan Sulfate ◽  
Vocal Folds ◽  
Basement Membrane Zone ◽  
Immunocytochemical Study ◽  
Frozen Sections ◽  
Intense Staining ◽  
Immunocytochemical Techniques

We evaluated the proteoglycan composition of normal vocal folds using immunocytochemical techniques. Frozen sections of 14 normal cadaveric vocal folds were obtained within 12 hours of death and sectioned immediately. Vocal fold sections were stained with antibodies against keratan sulfate, chondroitin sulfate, heparan sulfate proteoglycan (HSPG), decorin, and hyaluronate receptor. We found that the lamina propria has diffuse staining of fibrillar components with keratan sulfate and decorin. Intense staining was observed in the vocal ligament area with keratan sulfate. The HSPG was localized to the basement membrane zone. Chondroitin sulfate, HSPG, and hyaluronate receptor were detected in the cytoplasm of interstitial cells with immunocytochemical characteristics of macrophages. The keratan sulfate distribution suggests that fibromodulin may be significant in normal vocal folds. Production of HSPG and probably versican occurs in macrophages and fibroblasts in the lamina propria.

Collagen composite hydrogels for vocal fold lamina propria restoration

Biomaterials ◽  
2006 ◽  
Vol 27 (7) ◽  
pp. 1104-1109 ◽  
Author(s):  
Mariah S. Hahn ◽  
Benjamin A. Teply ◽  
Molly M. Stevens ◽  
Steven M. Zeitels ◽  
Robert Langer
Keyword(s):  
Lamina Propria ◽  
Vocal Fold ◽  
Composite Hydrogels

scholarly journals A method for identification of vocal fold lamina propria fibroblasts in culture

2008 ◽  
Vol 139 (6) ◽  
pp. 816-822 ◽  
Author(s):  
Susan L. Thibeault ◽  
Wenhua Li ◽  
Stephanie Bartley
Keyword(s):  
Lamina Propria ◽  
Cell Lines ◽  
Von Willebrand Factor ◽  
Vocal Fold ◽  
Fibroblast Cell ◽  
Cytokeratin 19 ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Area Of Study

Objective Vocal fold biology research is emerging as a vital area of study in laryngology. One impediment is the lack of both commercially available vocal fold lamina propria fibroblasts and a constitutively expressed specific marker for fibroblasts. We present an in vitro technique that allows for identification of fibroblasts by ruling out the possibility of the cells belonging to other lineages that are found in vocal fold tissue. Study Design An in vitro study. Methods Two primary vocal fold fibroblast cell lines and one immortalized vocal fold fibroblast cell line were cultured. Immunohistologic staining for α-actinin, cytokeratin 19, and von Willebrand factor was completed for the three fibroblast lines in addition to skeletal, endothelial, and epithelial cell lines. Cell type was differentiated by positive staining for α-actinin, cytokeratin 19, and von Willebrand factor. Results Fibroblast cultures did not express α-actinin, cytokeratin 19, and von Willebrand factor, whereas skeletal muscle, endothelial, and epithelial cultured cells expressed each respectively. Conclusions This simple rule-out methodology for fibroblast confirmation is an important step when establishing cell culture, and it establishes sound internal validity particularly in the early stages of this emerging area of study.

Vocal Fold Submucosal Infusion Technique in Phonomicrosurgery

1996 ◽  
Vol 105 (5) ◽  
pp. 341-347 ◽  
Author(s):  
Erik S. Kass ◽  
Robert E. Hillman ◽  
Steven M. Zeitels
Keyword(s):  
Lamina Propria ◽  
Retrospective Review ◽  
Vocal Fold ◽  
Malignant Lesions ◽  
Sulcus Vocalis ◽  
Infusion Technique ◽  
Superficial Lamina

Phonomicrosurgery is optimized by maximally preserving the vocal fold's layered microstructure (laminae propriae). The technique of submucosal infusion of saline and epinephrine into the superficial lamina propria (SLP) was examined to delineate how, when, and why it was helpful toward this surgical goal. A retrospective review revealed that the submucosal infusion technique was used to enhance the surgery in 75 of 152 vocal fold procedures that were performed over the last 2 years. The vocal fold epithelium was noted to be adherent to the vocal ligament in 29 of the 75 cases: 19 from previous surgical scarring, 4 from cancer, 3 from sulcus vocalis, 2 from chronic hemorrhage, and 1 from radiotherapy. The submucosal infusion technique was most helpful when the vocal fold epithelium required resection and/or when extensive dissection in the SLP was necessary. The infusion enhanced the surgery by vasoconstriction of the microvasculature in the SLP, which improved visualization during cold-instrument tangential dissection. Improved visualization facilitated maximal preservation of the SLP, which is necessary for optimal pliability of the overlying epithelium. The infusion also improved the placement of incisions at the perimeter of benign, premalignant, and malignant lesions, and thereby helped preserve epithelium uninvolved by the disorder.

scholarly journals Empirical measurements of biomechanical anisotropy of the human vocal fold lamina propria

2012 ◽  
Vol 12 (3) ◽  
pp. 555-567 ◽  
Author(s):  
Jordan E. Kelleher ◽  
Thomas Siegmund ◽  
Mindy Du ◽  
Elhum Naseri ◽  
Roger W. Chan
Keyword(s):  
Lamina Propria ◽  
Vocal Fold

scholarly journals Permeability of canine vocal fold lamina propria

2014 ◽  
Vol 125 (4) ◽  
pp. 941-945 ◽  
Author(s):  
Jacob P. Meyer ◽  
Anton A. Kvit ◽  
Erin E. Devine ◽  
Jack Jiang
Keyword(s):  
Lamina Propria ◽  
Vocal Fold

Posterior Glottis

1986 ◽  
Vol 95 (6) ◽  
pp. 576-581 ◽  
Author(s):  
Minoru Hirano ◽  
Kensuke Kiyokawa ◽  
Shigejiro Kurita ◽  
Kiminori Sato
Keyword(s):  
Lamina Propria ◽  
Vocal Fold ◽  
Posterior Part ◽  
Lateral Wall ◽  
Posterior Wall ◽  
Ciliated Epithelium ◽  
Posterior Glottis

Two sets of investigations were conducted with excised human larynges. 1) The glottis was closely observed and photographed from above and below in three conditions: neutral, adduction, and abduction. 2) The structure surrounding the posterior glottis was histologically investigated in the same three conditions. The results are as follows. 1) The structure surrounding the posterior glottis consists of three portions: the posterior wall of the glottis, the lateral wall of the posterior glottis, and the cartilaginous portion of the vocal fold. 2) During vocal fold adduction, the posterior part of the larynx closes completely not at the glottis but at the supraglottis, resulting in formation of a conic space in the posterior glottis that can be viewed only from below. 3) The posterior glottis accounts for approximately 35% to 45% of the entire glottic length and 50% to 65% of the entire glottic area. 4) The mucosa of the posterior glottis has ciliated epithelium. The lamina propria consists of two layers. 5) The posterior glottis can be regarded as a respiratory glottis.

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