Evaluation of Th17-related cytokines and IFNγproduction from blood mononuclear cells of moderate and severe asthmatic children reveals methylprednisolone does not decrease IL-22 levels

2014 ◽  
Vol 52 (3) ◽  
pp. 227-231 ◽  
Author(s):  
Emanuel Sávio Cavalcanti Sarinho ◽  
Adriana Azoubel-Antunes ◽  
Moacyr Jesus Barreto de Melo Rêgo ◽  
Mariana Brayner-Cavalcanti ◽  
Thiago Ubiratan Lins e Lins ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Asthmatic Children ◽  
Severe Asthmatic ◽  
Blood Mononuclear Cells

scholarly journals Mitochondrial Function in Peripheral Blood Mononuclear Cells (PBMC) Is Enhanced, Together with Increased Reactive Oxygen Species, in Severe Asthmatic Patients in Exacerbation

2019 ◽  
Vol 8 (10) ◽  
pp. 1613 ◽  
Author(s):  
Ederlé ◽  
Charles ◽  
Khayath ◽  
Poirot ◽  
Meyer ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Mitochondrial Respiratory Chain ◽  
Ros Production ◽  
Oxygen Species ◽  
Respiratory Chain Complexes ◽  
Peripheral Blood Mononuclear ◽  
Asthmatic Patients ◽  
Chain Complexes ◽  
Severe Asthmatic ◽  
Blood Mononuclear Cells

asthma is a chronic inflammatory lung syndrome with an increasing prevalence and a rare but significant risk of death. Its pathophysiology is complex, and therefore we investigated at the systemic level a potential implication of oxidative stress and of peripheral blood mononuclear cells’ (PBMC) mitochondrial function. Twenty severe asthmatic patients with severe exacerbation (GINA 4–5) and 20 healthy volunteers participated at the study. Mitochondrial respiratory chain complexes activities using different substrates and reactive oxygen species (ROS) production were determined in both groups by high-resolution respirometry and electronic paramagnetic resonance, respectively. Healthy PBMC were also incubated with a pool of plasma of severe asthmatics or healthy controls. Mitochondrial respiratory chain complexes activity (+52.45%, p = 0.015 for VADP) and ROS production (+34.3%, p = 0.02) were increased in asthmatic patients. Increased ROS did not originate mainly from mitochondria. Plasma of severe asthmatics significantly increased healthy PBMC mitochondrial dioxygen consumption (+56.8%, p = 0.031). In conclusion, such asthma endotype, characterized by increased PMBCs mitochondrial oxidative capacity and ROS production likely related to a plasma constituent, may reflect activation of the immune system. Further studies are needed to determine whether increased PBMC mitochondrial respiration might have protective effects, opening thus new therapeutic approaches.

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