Enzyme Immuno Assay of the Estrogen Receptor in Breast Cancer Biopsy Samples a Comparison with Isoelectric Focusing

e11609 Background: Impact of the mode of estrogen receptor determination on breast cancer prognosis Abstract Background: We examined if the mode of estrogen receptor (ER) determination and expression in primary breast cancer impact on patient's prognosis. Methods: We conducted an analysis on a prospective cohort of 1012 patients treated for stage I-III Breast Cancer at Conception Hospital, Marseille, France between 1984 to 2003. ER was determined according to enzyme immuno-assay (EIA) and immunohistochemistry (IHC). Disease-free and overall survivals were evaluated according to the mode of ER quantification and in function of qualitative or quantitative ER expression. Results: ER was a strong predictor of overall survival in the whole population whatever method used for the status determination (p<0.001); however when stratifying the analysis on ER quantitative expression, ER was still a prognostic indicator in the EIA group for OS (p= 0.004) and DFS (p<0.001), group but not in the IHC group for OS (p=0.58) and DFS (p= 0.195). Conclusions: ER determination in breast cancer is strongly associated with a better prognosis, but the level of influence of ER varies with the assay and ER expression. No significant financial relationships to disclose.

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High-Affinity Cytosol Binding Site(s) for Antiestrogen in Cell Lines Devoid of Estrogen Receptor and in Human Breast Cancer Biopsy Specimens

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1986.tb16046.x ◽

1986 ◽

Vol 464 (1 Endocrinology) ◽

pp. 496-498

Author(s):

CLAIRE CHOUVET ◽

SIMONE SAEZ

Keyword(s):

Breast Cancer ◽

Estrogen Receptor ◽

Binding Site ◽

Cell Lines ◽

Human Breast Cancer ◽

Human Breast ◽

High Affinity ◽

Biopsy Specimens ◽

Cancer Biopsy

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Application of EM to differentiate herpes virus from pox virus in genital specimens

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169043 ◽

1994 ◽

Vol 52 ◽

pp. 262-263

Author(s):

K. Rekrut ◽

K. Schleuter

Keyword(s):

Tissue Culture ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Fresh Tissue ◽

Culture Cells ◽

Viral Transport ◽

Carbon Coated ◽

Immuno Assay ◽

Simplex Virus ◽

Enzyme Immuno Assay

Confirmation of herpes simplex virus (HSV) from genital lesions of obstetrical (OB) patients may affect both the management of the delivery and of the neonate.(l,2) During 1992 and 1993, 4,450 genital specimens from OB patients were submitted in viral transport media for herpes culture. The specimens were inoculated into MRC-5, Vero, and A-549 tissue culture tubes, incubated, and examined daily for 7 days for cytopathic effect (CPE). The original specimens were frozen at −70° C until final reports were issued. Culture tubes with CPE were tested by the Dupont Herpchek enzyme immuno assay (EIA) to confirm the presence of herpes simplex virus (HSV). (3,4) 170 OB patient specimens were positive by culture and confirmed by EIA.There were also 63 cultures exhibiting CPE ressembling HSV which were negative by EIA testing, which failed to pass in fresh tissue culture cells or progress to more enhanced CPE in culture. These original specimens were screened by electron microscopy after direct ultracentrifugation employing the Beckman airfuge with the EM 90 rotor on to formvar carbon-coated 300 mesh copper grids and negatively stained with 2% PTA.

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