Microwave Methods for Reducing Staining Time to Seconds

1987 ◽  
Vol 62 (5) ◽  
pp. 357-360 ◽  
Author(s):  
Hendrik E. Moorlag ◽  
Mathilde E. Boon ◽  
L. P. Kok
Keyword(s):  
Staining Time

scholarly journals Agarose beads as matrices for proteins in cytophotometric investigations of immunohistoperoxidase procedures;.

1975 ◽  
Vol 23 (4) ◽  
pp. 243-250 ◽  
Author(s):  
J G Streefkerk ◽  
M van der Ploeg ◽  
P van Duijn
Keyword(s):  
Model System ◽  
Suitable Model ◽  
Protein Marker ◽  
Agarose Beads ◽  
Immunologic Reactions ◽  
Final Amount ◽  
Staining Time

Quantitative aspects of direct immunohistoperoxidase procedures were studied in a model system consisting of agarose beads to which antigens or antibodies had been coupled. It could be proven that the final amount of reaction product resulting from the histoperoxidase reaction with 3,3-diaminobenzidine-tetra HCl in a bead was linearly related to the volume of the beads and to the staining time. This implies that protein-coupled agarose beads are a suitable model for the study of stoichiometric aspects of immunologic reactions in immunohistochemistry as well as in general immunologic methods when peroxidase is used as the protein marker.

Rapid Lipid-Staining Procedure for Paper Electrophoretograms

1973 ◽  
Vol 19 (1) ◽  
pp. 106-108 ◽  
Author(s):  
Herbert K Naito ◽  
Lena A Lewis
Keyword(s):  
Staining Technique ◽  
Staining Procedure ◽  
Acetone Water ◽  
Oil Red O ◽  
Staining Time

Abstract We describe a rapid, reliable staining procedure for lipoproteins on paper electrophoretograms. Staining time, is 2.5 h in an acetone-water (1:1, by vol) solution of Oil Red O dye (0.4 g/liter). Results agree well with those obtained by the conventional overnight staining technique

scholarly journals DIAZO-SAFRANIN FOR STAINING ENTEROCHROMAFFIN

1953 ◽  
Vol 1 (3) ◽  
pp. 154-159 ◽  
Author(s):  
R. D. LILLIE ◽  
H. J. BURTNER ◽  
J. P. GRECO HENSON
Keyword(s):  
Sodium Nitrite ◽  
Coupling Reactions ◽  
Enterochromaffin Cell ◽  
Sodium Nitrite Solution ◽  
Black Background ◽  
M 10 ◽  
Background Staining ◽  
Safranin O ◽  
Staining Time

Freshly diazotized safranin O colors enterochromaffin cell granules black. Background staining can be kept down to a fairly light red. Fairly stable acid aqueous safranin solution and normal sodium nitrite solution can be kept on hand and mixed ex tempore to prepare the diazo solution. A diazotization time of 15 minutes at 3°C is adequate, and the coupling (staining) time of 5 minutes should not he exceeded. Both the specific and the background stains are acid fast. A 1:40 dilution in M/10 Na2HPO4 produces the desired grade of alkalinity and a proper dilution for best. contrast. In vitro alkaline coupling reactions with diazo-safranin produced colored precipitates with phenol, cresols, naphthols, resorcinol, hydroquinone, pyrogallol and tyrosine. Definite colors were produced on gelatin paper impregnated with resorcinol and the naphthols, less definite with phenol and the cresols. Gelatin paper models gave positive reactions with diazotized α-naphthylamine for catechol, pyrogallol and phloroglucinol as well as for resorcinol and the naphthols. The positive coupling with diazo-safranin speaks against a catechol or hydroquinone structure and supports the thesis of a resorcinol structure.

Mechanism of connective tissue techniques I. The effect of dye concentration and staining time on anionic dye procedures

1993 ◽  
Vol 25 (11) ◽  
pp. 821-829 ◽  
Author(s):  
P. E. Reid ◽  
M. Iagallo ◽  
S. Nehr ◽  
M. Jankunis ◽  
L. Morrow ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Anionic Dye ◽  
Staining Time

scholarly journals The Use of Natural Dyes from Beetroot Skin Extract (Beta Vulgaris) as Teaching Material on Cell Division for Senior High School Students

2019 ◽  
Vol 2 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Adilla Nur Azizah ◽  
Putri Agustina ◽  
Suparti Suparti ◽  
Alanindra Saputra ◽  
Yasir Sidiq
Keyword(s):  
High School ◽  
Beta Vulgaris ◽  
Allium Cepa ◽  
Plant Tissue ◽  
Senior High School ◽  
Natural Dyes ◽  
Skin Extract ◽  
Synthetic Dyes ◽  
Staining Time

One of the Basic Competencies (KD) in Biology for Indonesian Senior High School is KD 4.12 which reads "Presents observations of the process of mitosis in the onion root and determines the phases it finds". Based on the basic competency test, students are required to have skills in making and observing mitotic preparations of plant tissue with at least using onion. Observation of mitotic division in plant tissues requires skill in making preparations. Making preparations in observing cells and tissues of plants or animals needs staining. Dyes often used in lab work are synthetic dyes. One of the synthetic dyes commonly used is safranin. However, safranin is quite expensive. This limitation requires teachers to look for alternatives to synthetic dyes that are easily found at a more affordable price, namely with natural dyes. One of the plants that has the potential to overcome this issue is beetroot (Beta vulgaris). The purpose of this study was to determine the quality of plant tissue preparations (Allium cepa) stained with beetroot skin (Beta vulgaris) as a natural coloring agent. The research method used was an experimental method with a Completely Randomized Design consisting of two factors, namely the staining time (1 hour, 2 hours, and 3 hours) and the type of solvent (aquades and citric acid 10%). The results of the study were analyzed with qualitative descriptive methods which included contrast and clarity of the preparations. Based on the results of the study, the quality of Allium cepa root preparations using beetroot (Beta vulgaris) skin extract with variations in the type of solvent and duration of staining showed good results on the staining time of 1 hour and 2 hours. The staining time of the preparations can affect the contrast and clarity of the preparations.

scholarly journals SIMULTANEOUS DIFFERENTIAL STAINING BY A CATIONIC CARBOCYANINE DYE OF NUCLEIC ACIDS, PROTEINS AND CONJUGATED PROTEINS II. CARBOHYDRATE AND SULFATED CARBOHYDRATE-CONTAINING PROTEINS

1974 ◽  
Vol 22 (8) ◽  
pp. 774-781 ◽  
Author(s):  
MARIE R. GREEN ◽  
JULLIA V. PASTEWKA
Keyword(s):  
Mast Cells ◽  
Nucleic Acids ◽  
Hydrogen Ion ◽  
Differential Staining ◽  
Ion Concentrations ◽  
Pathologic Diagnosis ◽  
Carbocyanine Dye ◽  
Sulfated Carbohydrate ◽  
Color Reactions ◽  
Staining Time

The cationic carbocyanine dye l-ethyl-2-[3-(l-ethylnaphtho[l,2d]thiazolin-2-ylidene)-2-methylprolpenyl]-naphtho[1, 2d]thiazolium bromide stains several classes of macromolecules differentially in histologic sections. Most proteins are red at pH 4.3 and pink or unstained at pH 2.8. The caseins are blue at pH 4.3 and unstained at pH 2.8. Nuclei stain purple, mast cells stain red-purple, cartilage stains purple and mucoproteins stain blue-green at both hydrogen ion concentrations. The nature of some of the macromolecules involved in these color reactions has been determined by the use of chemical and enzymatic procedures and by staining films of glycosaminoglycuronoglycans, proteins and conjugated proteins. The method requires less than 1 hr staining time for most tissues; the stain is stable when kept in the dark and has the advantage of distinguishing several macromolecules in tissues simultaneously. The simplicity of the method and the ability to discriminate classes of macromolecules in tissues should make this stain valuable in pathologic diagnosis.

Sign in / Sign up

Export Citation Format

Share Document