scholarly journals Anticancer Activity of Vernonia amygdalina Del. Extract on WiDr Colon Cancer Cell Line

2018 ◽  
Author(s):  
Ramadhan Bestari ◽  
M. Ichwan ◽  
Mustofa ◽  
Denny Satria
Keyword(s):  
Colon Cancer ◽  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Vernonia Amygdalina

Colon cancer is the third most common cancer in men and second in women. Cancer treatments include chemotherapy, but some chemotheraphy drugs are resistant. Vernonia amygdalina Del. is one of the most widely reported plants that can inhibit the growth of various cancer cell lines. This study aims to examine the anticancer activity of Vernonia amygdalina Del. extract on WiDr colon cancer cell line. This research is an experimental research in vitro using posttest-only control group design. Research subjects are WiDr cells. The research method starts from preparation and characterization of simplicia, gradual maceration of Vernonia amygdalina Del. using n-hexane, ethyl acetate, and ethanol, phytochemical analysis of each extract, cytotoxicity assay on WiDr cells using MTT (Microculture Tetrazolium Technique) assay. Ethyl acetate extract of Vernonia amygdalina Del. has the lowest IC50 value in cytotoxicity assay results, indicate that ethyl acetate extract has very strong citotoxic activity on WiDr cells. Further studies are to analyze the mechanisms of ethyl acetate extracts in induce apoptosis, inhibition of cell cycle, and to observe the protein expression associated with it.

scholarly journals Evaluation of the Potential Anticancer Activity of Zamzam Water in Human Colon Cancer Cell Line

2016 ◽  
Vol 4 (3) ◽  
pp. 33-41 ◽  
Author(s):  
Huda A. Al Doghaither ◽  
Ayat B. Al-Ghafari ◽  
Sawsan A. Rahimulddin ◽  
Shiekhah M. Al Zahrani ◽  
Abdulkader M. Shaikh Omar ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Human Colon ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer

scholarly journals Cytotoxicity and Antiproliferation of Phycocyanin from Spirulina platensis Extract on WiDr Colon Cancer Cell Line

2020 ◽  
Vol 12 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Ajeng Kurniasari Putri ◽  
Safira Chairani Dimarti ◽  
Renni Yuniati ◽  
Neni Susilaningsih
Keyword(s):  
Colon Cancer ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Spirulina Platensis ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Inhibition Of Proliferation

Phycocyanin from Spirulina platensis extract has anticancer activity against various types of cancer cell cultures. However study about its effect on colon cancer cell lines, especially the WiDr, has not been reported before. This study aimed to reveal the anticancer activity of phycocyanin from Spirulina platensis extract on WiDr cells. The research was an in vitro experimental study, with the investigation on cytotoxicity also antiproliferation as the anticancer parameters. Both cytotoxicity and antiproliferation test was conducted through MTT assay to observe the visualization and inhibition of proliferation of different concentrations of phycocyanin in several incubation times on the WiDr colon cancer cell line. The obtained data were then processed statistically with the Two Way ANOVA test at a significance value of p <0.05 and followed with the Post Hoc test since there were significant differences. Based on the results, it could be postulated that phycocyanin extracted from freshwater Spirulina platensis was classified as non-toxic (IC50 of 855 µg/ml). Consequently, it is less potential to be used as the treatment for colon cancer. However, phycocyanin could inhibit the proliferation of the WiDr cell for approximately 47.4%, specifically at the concentration of 1710 µg/ml for 72 hours. It could be concluded that freshwater phycocyanin is less effective as an anticancer substance. The benefit of this study is to provide the new scientific evidence of the contrary results of freshwater phycocyanin activity from Spirulina platensis as an anticancer agent of colon cancer.

Anticancer effect of X-Ray triggered methotrexate conjugated albumin coated bismuth sulfide nanoparticles on SW480 colon cancer cell line

2020 ◽  
Vol 582 ◽  
pp. 119320
Author(s):  
Mohammad Hasan Faghfoori ◽  
Hamed Nosrati ◽  
Hamed Rezaeejam ◽  
Jalil Charmi ◽  
Saeed Kaboli ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Bismuth Sulfide ◽  
Anticancer Effect ◽  
X Ray

scholarly journals Target disruption of the mutant β-catenin gene in colon cancer cell line HCT116: preservation of its malignant phenotype

Oncogene ◽  
2002 ◽  
Vol 21 (38) ◽  
pp. 5906-5911 ◽  
Author(s):  
Shigeki Sekine ◽  
Tatsuhiro Shibata ◽  
Michiie Sakamoto ◽  
Setsuo Hirohashi
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Malignant Phenotype

scholarly journals In situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Line

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jigang Wang ◽  
Jianbin Zhang ◽  
Chong-Jing Zhang ◽  
Yin Kwan Wong ◽  
Teck Kwang Lim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Proteomic Profiling ◽  
Hct116 Colon Cancer Cell

scholarly journals A new method for detection of tumor driver-dependent changes of protein sialylation in a colon cancer cell line reveals nectin-3 as TGFBR2 target

2015 ◽  
Vol 24 (10) ◽  
pp. 1686-1694 ◽  
Author(s):  
Jennifer Lee ◽  
Uwe Warnken ◽  
Martina Schnölzer ◽  
Johannes Gebert ◽  
Jürgen Kopitz
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
New Method
Sign in / Sign up

Export Citation Format

Share Document