scholarly journals In vitro seed germination in Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall. (Orchidaceae)

2010 ◽  
Vol 6 ◽  
pp. 100-102 ◽  
Author(s):  
Shreeti Pradha ◽  
Bijaya Pant
Keyword(s):  
Seed Germination ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Genetic Constitution ◽  
Ms Medium ◽  
Orchid Species ◽  
C Elegans ◽  
Protocorm Formation ◽  
Dendrobium Densiflorum

A comparative study of in vitro seed germination of two endangered orchid species, viz. Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall., was carried out on Murashige and Skoog's (MS) medium, supplemented with different concentrations and combination of 6-benzylaminopurine (BAP) and á-Naphthalene acetic acid (NAA). The hormone-free MS medium and MS medium supplemented with various growth hormones were found effective for in vitro seed germination of both species. However, the seeds of these two species showed variation in their germination behavior. Hormone-free MS basal medium was found most effective for seed germination of D. densiflorum; whereas, basal medium supplemented with BAP (1mg/l) was effective for C. elegans. The seeds of D. densiflorum showed quick response in earlier germination, protocorm formation and further development into seedlings in comparison to C. elegans. In C. elegans, germination of immature seeds started after nine weeks of inoculation; whereas in D. densiflorum, the initiation of germination started after five weeks of culture. The variations in seed germination, protocorm formation and seedling differentiation in the two orchid species might be due to the differences in their genetic constitution and the presence of different endogenous growth stimulating substances present in their seeds. The present study has provided useful information for in vitro clonal mass multiplication of these commercially important orchid species. Key-words: growth hormone; in vitro study; orchid.DOI: 10.3126/botor.v6i0.2917 Botanica Orientalis - Journal of Plant Science (2009) 6: 100-102

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

scholarly journals Establishment of an EfficientIn VitroRegeneration Protocol for Rapid and Mass Propagation ofDendrobium chrysotoxumLindl. Using Seed Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Potshangbam Nongdam ◽  
Leimapokpam Tikendra
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Germination Percentage ◽  
Naphthalene Acetic Acid ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Orchid Species ◽  
Seed Culture ◽  
Leaf Formation

An efficientin vitroregeneration protocol from seed culture has been established successfully forDendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal values. Seed germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins. Medium supplemented with 0.4% activated charcoal (AC), 2 mg/L 6-benzyl amino purine (BAP), and 2 mg/L indole-3-acetic acid (IAA) produced best seed germination percentage in 2 weeks of culture. Incorporation of higher concentration of kinetin (KN) or BAP in combination with low auxin in medium induced pronounced shooting and leaf formation. Reduction in leaf development was evident when cytokinins exist singly in medium indicating synergistic effect of auxin and cytokinin in leaf induction. Presence of elevated level of indole-3-butyric acid (IBA) or 1-naphthalene acetic acid (NAA) with low cytokinin content in medium generated morein vitrorooting, though IBA was found to be more effective in rooting induction as compared to NAA. Thein vitroprotocol for asymbiotic seed germination developed from the present investigation can be used for rapid mass propagation of this highly importantDendrobiumorchid species.

scholarly journals In vitro Micro Propagation of Soybean (Glycine max) BARI-5 Variety

2019 ◽  
Vol 13 (1) ◽  
pp. 177-187 ◽  
Author(s):  
Nadira Begum ◽  
Elina A. Zenat ◽  
Mohammad K.I. Sarkar ◽  
Chapol K. Roy ◽  
John L. Munshi ◽  
...  
Keyword(s):  
Glycine Max ◽  
Seed Germination ◽  
Agricultural Research ◽  
Average Length ◽  
Research Work ◽  
Naphthalene Acetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

Introduction: The present research work was undertaken with a view to developing a suitable protocol for in vitro plant regeneration of economically important plant (Glycine max) (Bangladesh Agricultural Research Institute BARI- 5) variety, via both direct and indirect organogenesis from in vitro grown seedlings. Methods: For micropropagation explants were cultured on MS and half strength Murashige and Skoog (MS) medium supplemented with various plant growth regulators (cytokinins and auxins). In the present study for inducting of callus, among 3 different hormone combinations, the suitable medium was 3.32 mg/L 2, 4-D containing MS medium and the callus was deep green in color. Different type of media like MS, 1/2 MS and MS with different (6-Benzyl Amino Purine) BAP concentration was used for seed germination of Glycine max. 100% of seed germination was observed in MS +1 mg/L BAP containing the medium. Results: In the present investigation, different concentration of cytokinins and auxins{BAP, 2, 4-D, and Naphthalene Acetic Acid (NAA)} were used individually or in combinations with MS medium to observe their effect on multiple shoot regeneration from the cotyledonary nodal segment. 100% shoot formation from cotyledonary nodal segment was recorded in 1.5 mg/L BAP and 0.15 mg/L BAP + 0.025 mg/L NAA containing MS medium, the best number of shoot was 10.9±2.0 found in MS + 1.5 mg/L BAP containing medium and highest length of shoot was 2 cm recorded in 1.5 mg/L BAP + 0.3 mg/L (different concentrations of Giberrellic acid) GA3 containing MS medium. In addition, for root induction in vitro raised well developed and elongated shoots were excised and cultured on MS and 1/2 MS medium supplemented with various concentration of Indole-3-Butyric acid (IBA). It was observed that MS medium containing 0.1 mg/L IBA and 1/2 MS medium containing 0.25 mg/L IBA was optimal for root induction. In which 100% shoots rooted well within 13 days of culture. The highest average number of roots per shoot was 6 recorded in MS +0.5 mg/L IBA containing the medium and highest average length of root was 8 cm recorded in 0.1 mg/L IBA containing MS medium. Conclusion: The most effective surface sterilization treatment for explants of Glycine max has been found in 0.1% HgCl2 solution for 15 minutes.

scholarly journals МОНГОЛ ОРНЫ ЭНДЕМИК УРГАМАЛ МОНГОЛ ДОГАР-CARYOPTERIS MONGOLICA BGE.-ИЙГ IN VITRO НӨХЦӨЛД ҮРЖҮҮЛСЭН ДҮНГЭЭС

2014 ◽  
Vol 52 (2) ◽  
pp. 23-28
Author(s):  
Д. Бямбасүх
Keyword(s):  
Acetic Acid ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Nodal Segment ◽  
Ms Medium ◽  
Endemic Plant ◽  
Shoot Height ◽  
Cotyledon Explants ◽  
Multiple Buds

Caryopteris mongolica Bge. буюу Монгол Догарын үрийг ариутгаад гиббереллиний хүчил(ГХ) 0.5мг/л, 1мг/л, 2мг/л тус тус агуулсан болон дан МС(хяналт) тэжээлт орчин дээр соёолуулсан. ГХ 2мг/л агуулсан хувилбар нь хяналттай харьцуулахад 20 хувиар үрийн соёололтыг нэмэгдүүлсэн. 28 хоногтой цухуйцаас хажуугийн нахиа бүхий ишний үе болон үрийн талын хэсгийг эксплант болгон сонгон авч Бензиламинопурин(БАП) 1мг/л, БАП 2мг/л ба Индол-3-цууны хүчил(ИЦХ) 0.3 мг/л, БАП 3мг/л концентрациар тус тус агуулсан, мөн Кинетин(КИН) 1мг/л, КИН 2мг/л ба α-нафталин цууны хүчил(НЦХ) 0.3мг/л, КИН 3мг/л ба НЦХ 0.4мг/л харьцаагаар тус тус агуулсан МС үндсэн тэжээлт орчинд өсгөвөрлөсөн. 21 хоногийн дараа найлзуур ургаж, нахиа олширсон байсан учир хэмжилт авч, 28 хоногийн дараа субкультур хийсэн. Нахиа үүсгэхэд хамгийн тохиромжтой орчны хувилбараар үрийн талын эксплант дээр 3 мг/л БАП, харин нахиа бүхий ишний үеийн эксплант дээр КИН=3мг/л, НЦХ=0.4мг/л өсөлтийн бодис агуулсан МС тэжээлт орчин байв. Үүссэн найлзууруудаа ямар нэг өсөлтийн бодис агуулаагүй ½ МС тэжээлт орчинд өсгөвөрлөхөд 7-10 хоногийн дараа үндэс үүсч эхэлж байсан бөгөөд 21-28 хоногт 95% нь үндэслэж байв.IN VITRO PROPAGATION THE MONGOLIAN ENDEMIC PLANT CARYOPTERIS MONGOLICA BGE.Caryopteris mongolica Bge. is rare and endemic plant of Mongolia, which used in traditional medicine of various diseases. We have initiated in vitro propagation the Caryopteris mongolica Bge. from seeds. Seeds were surface sterilized by immersing in 70% ethanol for 90 sec, 10% hydro peroxide for 10 minutes and 5% sodium hypochlorite for 20 minutes. Finally, rinsed with sterile distilled water for 4 times. Sterilized seeds were germinated on MS medium supplemented with 0.5, 1, 2 mg/l gibberillic acid, respectively. Nodal segment and cotyledon explants from 4 weeks old seedling were cultured on the MS basal medium supplemented with benzylaminopurine(BAP) 1 mg/l, BAP 2 mg/l and Indole acetic acid(IAA) 0.3 mg/l, BAP 3 mg/l, Kinetin(KIN) 1 mg/l, KIN 2 mg/l and Naphthalene acetic acid(NAA) 0.3 mg/l, KIN 3 mg/l and NAA 0.4 mg/l concentrations, respectively. After 3 weeks, measured of shoot height and bud number and 4 weeks later subcultured in a same medium. High frequency multiple buds were formed on MS medium supplemented with BAP 3mg/l, from cotyledon explants, but KIN 3 mg/l and NAA 0.4 mg/l was best version of nodal segment explants. All shoots were cultured in the ½ MS basal without any growth regulators for induction of root. After 7-10 days, initiation of root form and 21-28 days later 95% of cultured shoots were rooted.Key words: Caryopteris mongolica Bge., MS basal medium, Gibberillic acid, Benzylaminopurine, Kinetin, Indole-3-acetic acid, α-Naphthalene acetic acid, explants.DOI: http://dx.doi.org/10.5564/pmas.v52i2.356 Proceedings of the Mongolian Academy of Sciences Vol.52(2) 2012 p.23-28

scholarly journals Micropropagation of Baptisia `Purple Smoke'

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 353-354 ◽  
Author(s):  
James R. Ault ◽  
Kayri Havens
Keyword(s):  
Plant Growth Regulator ◽  
Potassium Salt ◽  
Shoot Growth ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Single Node ◽  
Shoot Initiation

Shoot explants from actively growing, greenhouse-maintained plants of Baptisia `Purple Smoke' were cultured in vitro for shoot initiation on Murashige and Skoog (MS) basal medium containing vitamins and supplemented with 30 g·L–1 sucrose, 8.87 μm BA, and 4.14 μm K-IBA. All subsequent media were supplemented with 2.47 mm NaH2PO4 to enhance shoot growth. Single-node explants were subcultured for shoot multiplication on MS medium with either no plant growth regulator or with 2.22, 4.44, 8.87, 17.74, or 35.48 mm BA in combination with 0.0 or 4.14 μm K-IBA. Explants produced a maximum of 4.1 shoots on the medium with 2.22 μm BA. Shoots rooted on all concentrations of K-IBA (2.07, 4.14, 10.36, or 20.72 μm) and K-NAA (2.23, 4.46, 11.15, or 22.29 μm) tested. Maximum rooting was 100% on MS medium with 11.15 μm K-NAA; however, this treatment induced copious stem callusing. Rooted shoots were greenhouse-acclimatized for 2.5 weeks. Overall survival was 86%. For optimal rooting and subsequent acclimatization, treatment with 2.23 μm K-NAA is recommended; this resulted in 83% rooting and 87% acclimatization. Chemical names used: N6 benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthalene acetic acid (K-NAA).

scholarly journals Comparison of Phytochemical and Antioxidant Activities in Micropropagated and Seed-derived Salvia miltiorrhiza Plants

HortScience ◽  
2018 ◽  
Vol 53 (7) ◽  
pp. 1038-1044 ◽  
Author(s):  
Bo-Ling Liu ◽  
Zhi-Bin Fan ◽  
Ze-Qun Liu ◽  
Xun-Hong Qiu ◽  
Yan-Hong Jiang
Keyword(s):  
Seed Germination ◽  
Antioxidant Activities ◽  
Salvia Miltiorrhiza ◽  
In Vitro Regeneration ◽  
Antioxidant Properties ◽  
Adventitious Shoots ◽  
Chemical Properties ◽  
Naphthalene Acetic Acid ◽  
Ms Medium

Salvia miltiorrhiza (commonly known in China as Danshen) is widely used in traditional Chinese medicine, and it is applied in the treatment of many diseases, particularly cardiovascular disease. Commercial propagation of Danshen is carried out either through seed germination or in vitro regeneration (micropropagation). However, it is not clear if the different propagation methods affect the chemical properties of the derived plants. In the present study, we first established a highly efficient tissue culture system for Danshen propagation. The addition of 1.0 mg·L−1 6-benzyladenine (BA) and 0.1 mg·L−1 α-naphthalene acetic acid (NAA) to Murashige and Skoog (MS) medium was optimal for inducing adventitious shoots; the highest rate of rooting was recorded on MS medium with 0.2 mg·L−1 NAA, on which the survival rate of transplanted plantlets was 95%. Next, we assessed antioxidant properties in the different tissues of plants of the same age, derived from micropropagation or seed germination, and measured tanshinone, total phenol, and total flavonoid contents. Our results showed that tissues of micropropagated plantlets had higher antioxidant activities than tissues of seed-derived plantlets; the micropropagated plantlets also had higher tanshinone contents in their roots. Thus, a rapid and efficient micropropagation system was established for Danshen, and it can be used for cultivating this plant to obtain therapeutic compounds.

Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants

2012 ◽  
Vol 7 (4) ◽  
pp. 698-707 ◽  
Author(s):  
Ely Zayova ◽  
Ira Stancheva ◽  
Maria Geneva ◽  
Maria Petrova ◽  
Rumiana Vasilevska-Ivanova
Keyword(s):  
Acetic Acid ◽  
Basal Medium ◽  
Echinacea Purpurea ◽  
Water Soluble ◽  
Cultivated Plants ◽  
Naphthalene Acetic Acid ◽  
High Survival ◽  
Ms Medium

AbstractAn effective in vitro protocol for rapid clonal propagation of Echinacea purpurea (L.) Moench through tissue culture was described. The in vitro propagation procedure consisted of four stages: 1) an initial stage - obtaining seedlings on Murashige and Skoog (MS) basal medium with 0.1 mg L−1 6-benzylaminopurine, 0.1 mg L−1 α-naphthalene acetic acid and 0.2 mg L−1 gibberellic acid; 2) a propagation stage — shoot formation on MS medium supplemented with 1 mg L−1 6-benzylaminopurine alone resulted in 9.8 shoots per explant and in combination with 0.1 mg L−1 α-naphthalene acetic acid resulted in 16.2 shoots per explant; 3) rooting stage — shoot rooting on half strength MS medium with 0.1 mg L−1 indole-3-butyric acid resulted in 90% rooted microplants; 4) ex vitro acclimatization of plants. The mix of peat and perlite was the most suitable planting substrate for hardening and ensured high survival frequency of propagated plants. Significant higher levels were observed regarding water-soluble and lipid-soluble antioxidant capacities (expressed as equivalents of ascorbate and α-tocopherol) and total pnenols content in extracts of Echinaceae flowers derived from in vitro propagated plants and adapted to field conditions in comparison with traditionally cultivated plants.

scholarly journals Perkecambahan dan Perbanyakan Gaharu secara In Vitro

2016 ◽  
Vol 1 (2) ◽  
pp. 62
Author(s):  
Mia Kosmiatin ◽  
Ali Husni ◽  
Ika Mariska
Keyword(s):  
Seed Germination ◽  
Nicotinic Acid ◽  
Germination Rate ◽  
Basal Medium ◽  
Ms Medium ◽  
Shoot Induction ◽  
Single Node ◽  
Fungal Inoculum ◽  
Aquilaria Malaccensis

<p class="p1">Agarwood (<em>Aquilaria malaccensis </em>Lank) is one of the forest wood that are continously exploited. Currently, the Indonesian export of agarwood is decreasing because its population is endangered by excessive logging. Agarwood propagations need technology for reproduction of agarwood seedlings and their fungal inoculum. <em>In vitro </em>technique for germination of recalsitrant seeds and micropropagation are technologies that can be used for propagation of agarwood seedlings. An experiment was done to develop techniques for <em>in vitro </em>germination and micropropagation of agarwood. The <em>in vitro </em>germination was done using two different techniques. Firstly, sterile seeds were germinated on an MS medium + 50 mg/l PVP, 50 mg/l GA, and 1 mg/l BA or kinetin. Secondly, sterile seeds were germinated on basal medium of MS, 1/2 MS medium, MS medium without vitamins, as well as on MS medium without pyridoxine, nicotinic acid and WPM. Shoot initiations and multiplications were done on MS and 1/2 MS media containing 1, 3, or 5 mg/l BA. The explants used were cotyledone nodes, terminal shoots, single node with leaf, and sinle node without leaf. The results showed that the seed germination rate on the different media ranged from 7,14 to 50%. The seed germination rate on the MS medium without vitamis was the highest. The best explants for shoot induction and multiplication was single node with leaf which was cultured on MS + 1 mg/l BA.</p>

scholarly journals In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species

2020 ◽  
Vol 12 (1) ◽  
pp. 74-89
Author(s):  
Michael S. AKINROPO ◽  
Benjamin E. AYISIRE ◽  
Ejeoghene R. OGBIMI
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Cotyledon Explants ◽  
Enterolobium Cyclocarpum

This study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination.  The leaf explants did not respond to these treatments.  The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal > stem > cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus.  The highest callus production was observed on MS medium supplemented with 2.7 µM NAA + 2.2 µM BA and 5.4 µM NAA alone.  Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 µM BA+2.7 µM NAA and 2.2µM BA+2.7 µM NAA respectively.  On the basal medium, 10% of the excised shoots rooted successfully. Thus, this in vitro method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies.

scholarly journals Micropropagation to Conserve the Endangered Gabal Elba Dragon Tree (Dracaena ombet Heuglin ex Kotschy & Peyr)

HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 162-166
Author(s):  
Yaser Hassan Dewir ◽  
Abdulhakim A. Aldubai ◽  
Rashid Sultan Al-Obeed ◽  
Salah El-Hendawy ◽  
Mayada Kadri Seliem ◽  
...  
Keyword(s):  
Seed Germination ◽  
In Vitro Propagation ◽  
Naphthalene Acetic Acid ◽  
Endangered Plant ◽  
Ms Medium ◽  
Axillary Shoots ◽  
Simple Method ◽  
Indole Butyric Acid ◽  
Dracaena Ombet

Plant tissue culture offers opportunities for the rescue and conservation of endangered plant species. Here, we report the successful in vitro propagation of Dracaena ombet, an endangered plant. Several physical and chemical seed treatments were evaluated to develop a propagation approach. Germination of D. ombet seeds was monitored for 16 weeks by placing them onto Murashige and Skoog (MS) medium. Maximum seed germination (20%) was recorded when seeds were soaked-scarified, whereas all other treatments did not result in seed germination. Fragmented (longitudinally bisected) and intact in vitro shoots were cultured onto MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) and indole butyric acid (IBA) to induce axillary shoots. Longitudinal fragmentation of explants had a greater effect than the intact explants for shoot proliferation when cultured onto medium containing plant growth regulators. Fragmented shoots cultured onto MS medium supplemented with 2 mg·L−1 BAP and 0.5 mg·L−1 IBA treatment resulted in the highest amount of axillary shoots (seven shoots per explant). The intact shoots had the highest axillary shoots (1.8 shoots per explant) when cultured onto a medium supplemented with a combination of 1 mg·L−1 BAP and 0.5 mg·L−1 IBA. One hundred percent rooting was obtained using half strength MS medium supplemented with 0.5 or 1 mg·L−1 IBA. With full strength MS medium, a maximum rooting of 60% was obtained with 1 mg·L−1 IBA or naphthalene acetic acid (NAA) addition. The plantlets were acclimatized to ex vitro conditions with a 95% survival rate. This study offers a simple method for in vitro propagation of D. ombet, which is valuable to enable conservation of this endangered species.

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