scholarly journals Establishment of an Efficient Regeneration System Amenable to Agrobacterium Mediated Transformation of Two Elite Indica Rice Varieties of North East India

2015 ◽  
Vol 3 (4) ◽  
pp. 680-686 ◽  
Author(s):  
Mohitosh Dey ◽  
Sanjib Kumar Panda ◽  
Lingaraj Sahoo
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Indica Rice ◽  
Northeast India ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
Agrobacterium Mediated Transformation ◽  
North East

An efficient plant regeneration system from embryogenic callus of two elite indica rice (Oryza sativa spp. indica) varieties of Northeast India, Ketokijoha and Monoharsali is established. The effect of auxin, 2,4-dicholorophenoxy acetic acid (2,4-D) on callus induction was optimized. Friable, nodular and creamish-white embryogenic calli were induced from mature seeds on NB medium supplemented with 2.5 mg/l 2,4-D. Plants were regenerated from 40-50 days old embryogenic callus on NB medium containing 0.5 mg/l BAP (6-benzylaminopurine) and 0.25 mg/l ABA (abscisic acid). Regenerated plants with multiple tillers were rooted on half strength MS medium and rooted plants were acclimatized with 94% survival rate. Higher frequency of callus induction as well as plant regeneration was recorded in Ketokijoha as compared to Monoharsali. The calli of both the varieties were found amenable to Agrobacterium-mediated transformation as evident from strong GUS (β-glucuronidase) expression. The results may find wide application for genetic improvement for valuable traits these elite indica rice varieties of Northeast India. Int J Appl Sci Biotechnol, Vol 3(4): 680-686

scholarly journals Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)

2016 ◽  
Vol 5 (08) ◽  
pp. 1395 ◽  
Author(s):  
Vijaya Naresh Juturu ◽  
Gopala Krishna Mekala ◽  
Mallikarjuna Garladinne ◽  
Puli Chandra Obul Reddy ◽  
Akila Chandra Sekhar*
Keyword(s):  
Callus Induction ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
Regenerated Plants ◽  
Rooting Medium

Though regeneration system in rice has been very well established compare to other crop plants, the fact remains that, most of the indica rice varieties are still recalcitrant for regeneration and genetic transformation. Therefore, refinement of tissue culture protocol for generation of embryogenic calli and regeneration of the fertile plants from a single cell should be a pre requisite event for development of transgenic plants. Here, in this study we reported high frequency robust regeneration protocols for a popular Indica cultivar Swarna.Mature seeds were used as initial material as explants. Highest callus induction % was observed in MSCIMP medium containing 2.0 mg-1 2,4, D + 0.5 mg-1 Kn as phytohormonal combinations. In addition, maximum regeneration was observed in 2.0 mg-l KN + 0.5 mg-l NAA. Regenerated plants were shifted to rooting medium followed by polyhouse for hardening. The callus induction and regeneration reported in this study were well suited for transformation agronomical important genes or functional genomics studies.

scholarly journals Embryogenic Callus Induction and Regeneration of Elite Bangladeshi Indica Rice Cultivars

1970 ◽  
Vol 17 (1) ◽  
pp. 65-70 ◽  
Author(s):  
ME Hoque ◽  
MS Ali ◽  
NH Karim
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Indica Rice ◽  
Rice Cultivars ◽  
Embryogenic Calli ◽  
Rice Callus ◽  
Embryogenic Callus Induction ◽  
Embryogenic Callus Formation

Significant variations were observed among six elite Bangladeshi Indica rice cultivars tested in relation to total callus induction frequency (p = 0.017), embryogenic callus formation frequency (p = 0.001) and subsequent plant regeneration responses (p = 0.005). In all the cases, embryogenic callus formation frequency was much more less than the total callus (embryogenic + non-embryonegic) formation frequency. The embryogenic calli derived from mature seed embryos produced green plants, successfully established in soil and produced fertile seeds.Key words: Indica rice, Callus induction, Plant regeneration, Genotypic variationsDOI = 10.3329/ptcb.v17i1.1122Plant Tissue Cult. & Biotech. 17(1): 65-70, 2007 (June)

scholarly journals Plant Regeneration Efficiency of Two Scented Indica Rice Varieties Pusa Basmati 1 and Kalanamak

2013 ◽  
Vol 22 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Aniruddha Kumar Tiwari ◽  
Md Shamim ◽  
Ravi Prakash Saxena ◽  
Kapil Deo Narayan Singh
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Indica Rice ◽  
Casein Hydrolysate ◽  
Growth Hormones ◽  
Rice Varieties ◽  
Shoot Initiation ◽  
Seed Embryo ◽  
Regeneration Efficiency

This study was undertaken to establish a regeneration protocol for two scented indica rice varieties, namely Pusa Basmati1 and Kalanamak. Callus culture in Pusa Basmati1 was initiated in MS containing 2.0 mg/l of 2,4-D. Optimum requirement of growth hormones for callus induction in Kalanamak was 1.5 mg/l of 2,4-D supplemented with 0.1 mg/l each of NAA and BAP in MS. Shoot regeneration in Pusa Basmati1 initiated in MS containing 2.0 mg/l BAP, 0.5 mg/l each of NAA and Kn. There was no shoot initiation in MS with same composition in Kalanamak. The shoot regeneration was successfully initiated and achieved in Dl medium supplemented with 2.0 mg/l BAP, 0.2 mg/l each of IAA, NAA and Kn, 500 mg/l each of proline and glutamine and 800 mg/l casein hydrolysate. The calli derived from mature seed embryo produced fertile green plants. The plants were successfully transferred to field with normal flowering. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14206 Plant Tissue Cult. & Biotech. 22(2): 163-169, 2012 (December)

scholarly journals Studies on In Vitro Response to Callus Induction and Regeneration of Five High Yielding Indica rice Varieties

2019 ◽  
Vol 7 ◽  
pp. 97-104
Author(s):  
Md. Niuz Morshed Khan ◽  
Md. Monirul Islam ◽  
Dr. Md. Shahidul Islam
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Indica Rice ◽  
Induction Medium ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
New Variety ◽  
Increasing Demand ◽  
Embryogenic Callus Formation

Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media composition. The highest callus induction were observed in media containing Sucrose as a carbon source. Among those varieties Binadhan-6 and BRRI dhan-48 showed highest rate of callus induction respectively. This study will be useful for selecting suitable callus induction medium for callus induction in future that will be useful for not only national but also international plant breeders for producing new variety and so on.

scholarly journals An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)

2019 ◽  
Vol 27 ◽  
pp. 89-99
Author(s):  
M Haque ◽  
SMS Islam
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Casein Hydrolysate ◽  
Ms Medium ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Hordeum Vulgare L ◽  
The Media ◽  
Barley Genotypes

This study was carried out to improve an efficient protocol for in vitro callus induction and plant regeneration using Bangladeshi barley genotypes collected from BARI, Gazipur, Bangladesh. After sterilization embryos were separated carefully from mature seeds of six barley genotypes (BB-1, BB-2, BB-3, BB-4, BB-5 and BB-6) and cultured them in MS medium supplemented with various concentration and combination of PGRs for callus induction and regeneration. Out of six genotypes BB-6 showed highest (38.17%) callus induction in MS + 4.0 mg/l 2,4-D + 200 mg/l L-proline + 300 mg/l casein hydrolysate; whereas, BB-4 and BB-5 showed no callus induction in the same medium. For plant regeneration from embryogenic calli the same genotype (BB-6) also performed the best results (19.25%) in MS medium supplemented with 1.5 mg/l BAP + 30 g/l sucrose. Analysis of variance (ANOVA) showed highly significant differences among the media and the genotypes. J. bio-sci. 27: 89-99, 2019

Potential Use of Partial Desiccation Treatment for Regeneration System of Malaysian Indica Rice (O. Sativa L.)

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Hazelynna Makerly ◽  
Zaidah Rahmat ◽  
Alina Wagiran
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Indica Rice ◽  
Rice Cultivar ◽  
Dichlorophenoxyacetic Acid ◽  
Regeneration System ◽  
Rice Callus ◽  
Regeneration Response ◽  
Partial Desiccation ◽  
2,4 Dichlorophenoxyacetic Acid

The present study shows that callus induction varied among the MR220 and MR232 cultivars tested and the response to 2,4–dichlorophenoxyacetic acid (2,4–D) was low. The highest percentage of callus induction for MR232 was 41% and 37% for MR220 cultivar. The morphology of embryogenic callus for both cultivars was dry, compact, and light yellowish. Partial desiccation treatment applied to improve plant regeneration of rice callus cultivars. Embryogenic rice callus was exposed to different desiccation periods (24 hours, 48 hours and 72 hours) and its effect was evaluated in terms of frequency of regeneration. The optimal desiccation period (72 hours) in MR232 callus enhanced plant regeneration response up to fivefold and twofold in MR220 rice cultivar (48 hours) compare to undesiccated control. The study suggests that partial desiccation treatment can be useful in stimulating regeneration response in indica rice particularly for Malaysian rice.

scholarly journals In vitro response and effect of gamma irradiation on four local indica rice varieties

2020 ◽  
pp. 90-92
Author(s):  
Md. Monirul Islam ◽  
Md Taufiqur Rahman ◽  
Md. Hasanuzzaman ◽  
Md. Shahidul Islam ◽  
Md. Imtiaz Uddin ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Gamma Radiation ◽  
Gamma Rays ◽  
Callus Induction ◽  
Indica Rice ◽  
Callus Growth ◽  
Rice Varieties ◽  
Rice Callus ◽  
In Vitro Response

In vitro response of four local Indica rice cultivars viz. Sadamota, Kachamota, Moulata and Dudhkalam was evaluated. The aim of this study is to develop an efficient protocol for callus induction, plant regeneration and to observe the effect of gamma radiation on plant regeneration for creating possible genetic variability. In Different concentration of 2,4-D and growth regulators were supplemented with MS medium (Murashige and Skoog’s) to observe their callus induction frequency using mature embryo as explant. Among the cutivars, the highest primary callus (92.55%) as well as embryogenic callus induction (56.26%) was showed in sadamota at 3.0 mgl-1 2,4-D and 10 mgl-1 kinetin under dark condition. Twenty one days old embryogenic calli were exposed to 0, 2, 4 and 6 Gy of gamma rays and transferred to regeneration medium. Both callus growth and regeneration capacity were found to be decreased with increasing level of exposure to gamma rays. The doses of 4 Gy of gamma radiation were found to be the 50% inhibition dose for callus growth and plant regeneration in sadamota and kachamota, repectively whereas the 50% inhibition dose for moulata and dudhkalam at 2 Gy. This results indicate that sentivity of gamma radiation on rice callus depends on genotype of a genus.

scholarly journals Studies on in vitro response to callus induction and gene transfer technique of five high yielding indica rice varieties.

1970 ◽  
pp. 41-45 ◽  
Author(s):  
Md. Niuz Morshed Khan ◽  
Dr. Md. Monirul Islam ◽  
Dr. Md. Shahidul Islam
Keyword(s):  
Gene Transfer ◽  
Callus Induction ◽  
Callus Formation ◽  
Indica Rice ◽  
Induction Medium ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
In Vitro Response ◽  
Increasing Demand

Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64were observed that is done in four different concentration of growth regulators (2,4-D). The highest callus induction were observed in media containing 2 mg/L 2,4-D. Among those varieties BRRI dhan-48 showed highest rate of callus induction. In this experiment Agrobacterium mediated gene transfer is also tried although plants were found nut need to be tested by molecular methods and tested the desired characters for selecting those plantlets as transgenic plant. This study will be useful for selecting suitable callus induction medium for callus induction in future.

scholarly journals PENGARUH JENIS AUKSIN TERHADAP PEMBENTUKAN KALUS DAN DAYA REGENERASI TIGA VARIETAS PADI LOKAL

2019 ◽  
Vol 2 (2) ◽  
pp. 77
Author(s):  
Zaiyin Rizky Ageng Maulidia ◽  
Wahyu Indra Duwi Fanata
Keyword(s):  
Genetic Diversity ◽  
Experimental Design ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Callus Induction ◽  
Plant Tissue ◽  
Transformation Techniques ◽  
Rice Varieties ◽  
Rice Genetic

ABSTRACT Indonesia is rich in rice genetic diversity in the form of javanica rice. Subspecies javanica have some superiority characters, among others a strong plant tissue, but in generally they have low productivity. Weaknesses of javanica rice can be repaired by biotechnology through genetic transformation techniques from callus of plant. Auxin types and genotypes are important factors for callus induction and plant regeneration. Therefore, this research held for know callus introduction and plant regeneration of three varieties of Javanica rice (Pendok, Genjah Arum, and Menthik Wangi Susu) combined with type of auxin (2,4-D and pychloram). The experimental design of RALs two factorials, there are three varieties of rice (Pendok, Genjah Arum, and Menthik Wangi Susu) and two types of auxin (2,4-D and Pychloram) and analyzed by DMRT α5%. The results of this study is Mentik Wangi Susu have highest regeneration than Pendok and Genjah Arum. The use of 2,4-D resulted in a higher callus induction and plant regeneration than pychloram in the three rice varieties used. While the best combination of treatments for callus induction and regeneration is Menthik Wangi Susu and 2,4-D. Keyword: auxin, javanica, callus induction, plant regeneration. ABSTRAK Indonesia kaya akan keragaman genetik padi berupa padi javanica. Padi sub spesies javanica memiliki beberapa keunggulan diantaranya jaringan tanaman yang kuat, namun pada umumnya mempunyai tingkat produktivitas gabah yang rendah. Kelemahan pada padi javanica dapat diperbaiki dengan bioteknologi melalui teknik transformasi genetik menggunakan jaringan kalus. Genotip dan jenis auksin merupakan faktor penting dalam induksi kalus dan regenerasi tanaman padi secara in vitro. Oleh karena itu, penelitian ini dilaksanakan untuk mengetahui daya pembentukan kalus dan regenerasi pada tiga varietas padi javanica, yaitu Pendok, Genjah Arum, dan Menthik Wangi Susu yang dikombinasikan dengan perlakuan jenis auksin berupa penggunaan 2,4-D dan pikloram. Rancangan percobaan yang digunakan adalah RAL dua faktorial yaitu varietas tanaman padi dan jenis auksin  dengan pengujian  menggunakan DMRT α5%. Hasil penelitian menunjukkan bahwa dari ketiga varietas padi yang digunakan, varietas Mentik Wangi Susu menunjukan daya regenerasi yang tertinggi dibandingkan varietas Pendok dan Genjah Arum.  Penggunaan 2,4-D menghasilkan tingkat pengkalusan dan regenerasi yang tinggi dibandingkan pikloram pada tiga varietas padi yang digunakan. Sedangkan kombinasi perlakuan yang terbaik untuk induksi kalus dan daya regenerasi adalah penggunaan varietas Menthik Wangi Susu dan auksin dalam bentuk 2,4-D.  Kata Kunci: auksin, javanica, induksi kalus, regenerasi tanaman. 

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