scholarly journals Characterization of Nepalese Barley Gene Pool for Leaf Rust Resistance

2016 ◽  
Vol 4 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Resham Babu Amgai ◽  
Sumitra Pantha ◽  
Madan Raj Bhatta
Keyword(s):  
Disease Resistance ◽  
Leaf Rust ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Gene Pool ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Puccinia Hordei ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Barley (Hordeum vulagare L) is the major crop for the people living in the high hills and mountainous region of Nepal. Leaf rust (caused by Puccinia hordei) is one of the major production threats for barley cultivation. A lot of variation can be observed on Nepalese barley accessions with respect to leaf rust resistance characteristics. Two hundred and forty one barley accessions were screened for leaf rust resistance characteristics on heading stage at Khumaltar, Lalitpur, Nepal. Among them, one hundred and nine Nepalese barley accessions showing promising for disease resistance were screened using six SSR markers linked to leaf rust resistance genes. Bonus and Local Jau was used as the resistant and susceptible check respectively. Leaf rust resistance genes Rph1, Rph2, Rph3, Rph7, QBLR-P and QTL on chromosome 5HS were detected on Nepalese barley accessions using respective SSR markers. Eight Nepalese barley accessions showed presence of three and more leaf rust resistant genes. The poor relationship between the field disease resistance and molecular markers linked with specific leaf rust resistance gene proved that Nepalese barley gene pool contains other leaf resistance genes.

scholarly journals Chromosomal Location and Genetic Relationship of Leaf Rust Resistance Genes Rph9 and Rph12 in Barley

1998 ◽  
Vol 88 (1) ◽  
pp. 76-80 ◽  
Author(s):  
I. G. Borovkova ◽  
Y. Jin ◽  
B. J. Steffenson
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Chromosomal Location ◽  
Leaf Rust Resistance ◽  
Chromosome 7 ◽  
Puccinia Hordei ◽  
Leaf Rust Resistance Gene ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Barley lines Hor 2596 and Triumph are the sources of leaf rust resistance genes Rph9 and Rph12, respectively. An allelism test was performed with F2 progeny of the cross Triumph/Hor 2596 inoculated with Puccinia hordei. No recombinants were found in a population of 3,858 progeny, indicating Rph9 and Rph12 are alleles. Molecular and morphological markers were used to identify the chromosomal location of these genes in the crosses Bowman/Hor 2596 and Triumph/I91-533-va. A linkage was detected between Rph9 and the flanking sequence-tagged site (STS) markers ABC155 and ABG3 on chromosome 7(5H) at a distance of 20.6 and 20.1 centimorgans (cM), respectively, and to the microsatellite marker dehydrin-9 (HVDHN9) at a distance of 10.2 cM in the Bowman/ Hor 2596 cross. Analysis of isozymes in bulks of the same population showed that Rph9 may be closely linked to the Est9 locus on chromosome 7(5H). The Rph12 locus was linked to the morphological trait locus va (controlling variegated leaf color) on chromosome 7(5H) at a distance of 22.6 cM in the Triumph/I91-533-va cross. Rph12 also was linked with STS marker ABC155 (24.4 cM) and RAPD marker OPA19 (1.5) (17.8 cM). These data indicate that Hor 2596 and Triumph carry a leaf rust resistance gene at the same locus on the long arm of chromosome 7(5H) of barley.

scholarly journals Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

Crop Science ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 282-302 ◽  
Author(s):  
Matthew J. Martin ◽  
Oswaldo Chicaiza ◽  
Juan C. Caffarel ◽  
Ahmad H. Sallam ◽  
Arnis Druka ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Introgression Lines ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

2019 ◽  
Vol 14 (1) ◽  
pp. 327-334 ◽  
Author(s):  
Agnieszka Tomkowiak ◽  
Roksana Skowrońska ◽  
Alicja Buda ◽  
Danuta Kurasiak-Popowska ◽  
Jerzy Nawracała ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Field Resistance ◽  
Wheat Cultivars ◽  
Pcr Conditions ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

Validating molecular markers for barley leaf rust resistance genes Rph5 and Rph7

2007 ◽  
Vol 126 (5) ◽  
pp. 458-463 ◽  
Author(s):  
J. A. Mammadov ◽  
W. S. Brooks ◽  
C. A. Griffey ◽  
M. A. Saghai Maroof
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

2013 ◽  
Vol 12 (9) ◽  
pp. 1580-1588 ◽  
Author(s):  
Li-hong SHI ◽  
Na ZHANG ◽  
Ya-ya HU ◽  
Xue-jun WEI ◽  
Wen-xiang YANG ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Chinese Spring Wheat ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes
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