Functional validation of plant transformation vector with stackedech42andbgnfromTrichodermain tomato for fungal disease resistance

ABSTRACT: Fungal diseases like early blight, late blight, fusarium wilt cause 30-40 per cent loss in fruit production. Form past decade many transgenic plants had been developed using genes encoding chitinases and glucanases with the objective of imparting fungal disease resistance. Since the genes encoding chitinase and glucanase act synergistically. The study was performed to construct plant transformation vector pRAGS carrying both ech42 and bgn under single T-DNA region. Initially, HindIII site at 5' end of earlier cloned bgn (T. harzianum) was removed using primers during reamplification of the gene. The amplicons were cloned into pTZ57R/T containing T overhangs at Eco321 site and transferred to E. coli DH5a and further to plant transformation vector pBI121 which was named as pRA121. In order to clone another gene (ech42) into pRA121, expression cassette from iHP vector was transferred to pRA121 and named as pRAG121. Further in order to gain XhoI site for cloning ech42 gene into pRAG121, ech42 (pSUM1) was cloned into pYES2/CT, named as pSAG1, ech42 from pSAG1 cloned with KpnI and XhoI in pRAG121 and named as pRAGS121. The vector constructed in the present study can be used to transform important crop plants to have enhanced resistance to fungal diseases.

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THE INTEGRATED PEAR DONORS WITH MONOGENIC DETERMINED STUNT

Vestnik of the Russian agricultural science ◽

10.30850/vrsn/2018/4/17-19 ◽

1970 ◽

pp. 17-19

Author(s):

Е. А. Dolmatov ◽

Т. А. Khrykina

Keyword(s):

Disease Resistance ◽

Russian Federation ◽

Fungal Disease ◽

Winter Hardiness ◽

Adaptive Potential ◽

Research Institutes ◽

Fungal Disease Resistance ◽

Sorting Process ◽

Red Coloration

Development of low-growing varieties is one of the prioritized directions in groups selection. Solution of excessive growth in the selection can be solved in today’s conditions by two means: on a polygenic and on a monogenic level. Up until recently such work was performed by research institutes of horticulture in the U.S.S.R. and Russian Federation only on the polygenic level. The analysis is performed for the data of 17 summer studies on the development of complex donors of monogenic determined dwarfness (gene D), high winter hardiness, group fungal disease resistance (scab, leaf spot and Septoria blight) and bright red coloration of pear fruits (gene C). On the first stage of these studies the issue of the development of population of hybrid dwarf types with high adaptive potential in the conditions of the Central Black Earth region of Russia was solved based on a hybridization of the donors of high winter hardiness and fungal disease resistance with the donors of monogenic determined dwarfness which were the descendants of 4th generation of the NainVert variety. As a result, several complex donors were selected. Its use in long-term pear selection programs would make sorting process possible on earlier stages of the ontogenesis and thanks to that would make it possible to halve the size of hybrid funds. Brief description of the complex donors is given.

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A study of the genetic diversity in the world soybean collection using microsatellite markers associated with fungal disease resistance

PROCEEDINGS ON APPLIED BOTANY GENETICS AND BREEDING ◽

10.30901/2227-8834-2020-3-81-90 ◽

2020 ◽

Vol 181 (3) ◽

pp. 81-90

Author(s):

A. K. Zatybekov ◽

Y. T. Turuspekov ◽

B. N. Doszhanova ◽

S. I. Abugalieva

Keyword(s):

Disease Resistance ◽

Ssr Markers ◽

Molecular Genetic ◽

Fungal Disease ◽

Molecular Genetic Analysis ◽

Fungal Diseases ◽

Fungal Disease Resistance ◽

Glycine Max L ◽

Purple Seed Stain ◽

Simple Sequence

Background. Soybean (Glycine max (L.) Merr.) gradually becomes one of the leading legume crops in Kazakhstan. The area under soybeans in the country has been increasing annually and requires the development of adapted cultivars with a higher yield, improved quality characters, and resistance to emerging fungal diseases. The enlargement of the crop’s gene pool also suggests the need to study and document local soybean accessions to meet the standards of the available world soybean collection by using reliable and informative types of DNA markers.Materials and methods. In this study, the soybean collection consisting of 288 accessions from different countries, including 36 cultivars and promising lines from Kazakhstan, was studied. The molecular genetic analysis was performed using nine polymorphic SSR (simple sequence repeats) markers, seven of which (Satt244, Satt565, Satt038, Satt309, Satt371, Satt570 and Sat_308) were associated with resistance to three main fungal diseases of soybean – frogeye leaf spot, fusarium root rot, and purple seed stain.Results. The average PIC (polymorphism information content) value of the analyzed SSR markers constituted 0.66 ± 0.07, confirming their highlevel polymorphism. The principal coordinate analysis suggested that the local accessions were genetically most close to the accessions from East Asia. As the collection showed a robust resistance to three studied fungal diseases in Almaty Region during 2018–2019, the distribution of the studied SSR markers in the population was not significantly associated with resistance to the analyzed diseases under field conditions.Conclusion. SSR genotyping of the soybean collection helped to identify accessions that potentially possess resistance-associated alleles of fungal disease resistance genes. The data obtained can be further used for the development of DNA documentation and the breeding the promising cultivars and lines of soybean.

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