scholarly journals DOUBLE-SIDED MICROPILLAR ARRAYS FOR CELL CONTRACTION FORCE MAPPING USING OPTICAL MOIRé METHOD

2014 ◽  
Author(s):  
F. Zhang ◽  
S.W. Anderson ◽  
X. Zheng3 ◽  
Y. Qiu4 ◽  
R. Liao4 ◽  
...  
Keyword(s):  
Contraction Force ◽  
Cell Contraction ◽  
Force Mapping ◽  
Moire Method ◽  
Micropillar Arrays

scholarly journals A novel collagen gel-based measurement technique for quantitation of cell contraction force

2015 ◽  
Vol 12 (106) ◽  
pp. 20141365 ◽  
Author(s):  
Tianrong Jin ◽  
Li Li ◽  
Richard C. M. Siow ◽  
Kuo-Kang Liu
Keyword(s):  
Kinase Inhibitor ◽  
Imaging System ◽  
Collagen Gel ◽  
Collagen Matrix ◽  
Collagen Gels ◽  
Contraction Force ◽  
Depth Sensing ◽  
Cell Contraction ◽  
Microscopy Imaging

Cell contraction force plays an important role in wound healing, inflammation, angiogenesis and metastasis. This study describes a novel method to quantify single cell contraction force in vitro using human aortic adventitial fibroblasts embedded in a collagen gel. The technique is based on a depth sensing nano-indentation tester to measure the thickness and elasticity of collagen gels containing stimulated fibroblasts and a microscopy imaging system to estimate the gel area. In parallel, a simple theoretical model has been developed to calculate cell contraction force based on the measured parameters. Histamine (100 µM) was used to stimulate fibroblast contraction while the myosin light chain kinase inhibitor ML-7 (25 µM) was used to inhibit cell contraction. The collagen matrix used in the model provides a physiological environment for fibroblast contraction studies. Measurement of changes in collagen gel elasticity and thickness arising from histamine treatments provides a novel convenient technique to measure cell contraction force within a collagen matrix. This study demonstrates that histamine can elicit a significant increase in contraction force of fibroblasts embedded in collagen, while the Young's modulus of the gel decreases due to the gel degradation.

NCX-1000, an NO derivative of UDCA, refeases NO and protects against portal hypertension by inhibiting hepatic stellate cell contraction

2001 ◽  
Vol 120 (5) ◽  
pp. A379-A379
Author(s):  
S FIORUCCI ◽  
E ANTONELLI ◽  
O MORELLI ◽  
A MENCARELLI ◽  
B PALAZZETTI ◽  
...  
Keyword(s):  
Portal Hypertension ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Cell Contraction

scholarly journals Wettability control of polymeric microstructures replicated from laser-patterned stamps

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yangxi Fu ◽  
Marcos Soldera ◽  
Wei Wang ◽  
Stephan Milles ◽  
Kangfa Deng ◽  
...  
Keyword(s):  
Contact Angle Hysteresis ◽  
Contact Angles ◽  
Spatial Period ◽  
Structured Surfaces ◽  
High Adhesion ◽  
Direct Laser ◽  
Periodic Microstructures ◽  
Structure Height ◽  
Direct Laser Interference Patterning ◽  
Micropillar Arrays

AbstractIn this study, two-step approaches to fabricate periodic microstructures on polyethylene terephthalate (PET) and poly(methyl methacrylate) (PMMA) substrates are presented to control the wettability of polymeric surfaces. Micropillar arrays with periods between 1.6 and 4.6 µm are patterned by plate-to-plate hot embossing using chromium stamps structured by four-beam Direct Laser Interference Patterning (DLIP). By varying the laser parameters, the shape, spatial period, and structure height of the laser-induced topography on Cr stamps are controlled. After that, the wettability properties, namely the static, advancing/receding contact angles (CAs), and contact angle hysteresis were characterized on the patterned PET and PMMA surfaces. The results indicate that the micropillar arrays induced a hydrophobic state in both polymers with CAs up to 140° in the case of PET, without modifying the surface chemistry. However, the structured surfaces show high adhesion to water, as the droplets stick to the surfaces and do not roll down even upon turning the substrates upside down. To investigate the wetting state on the structured polymers, theoretical CAs predicted by Wenzel and Cassie-Baxter models for selected structured samples with different topographical characteristics are also calculated and compared with the experimental data.

scholarly journals Smooth muscle cell contraction increases the critical buckling pressure of arteries

2013 ◽  
Vol 46 (4) ◽  
pp. 841-844 ◽  
Author(s):  
Danika M. Hayman ◽  
Jinzhou Zhang ◽  
Qin Liu ◽  
Yangming Xiao ◽  
Hai-Chao Han
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Contraction ◽  
Critical Buckling Pressure

Highly sensitive hair springs to measure the contraction force of engineered cardiac tissues

2020 ◽  
Vol 7 (5) ◽  
pp. 1327-1330 ◽  
Author(s):  
Qian-Ru Xiao ◽  
Si Sun ◽  
Nihad Cheraga ◽  
Kai-Hong Wu ◽  
Yong Jiang ◽  
...  
Keyword(s):  
Human Hair ◽  
Contraction Force ◽  
Cardiac Tissues ◽  
Highly Sensitive

Highly sensitive springs based on human hair are developed for measuring the contraction force of engineered cardiac tissues.

Effects of S1P on Myoblastic Cell Contraction: Possible Involvement of Ca2+-Independent Mechanisms

2004 ◽  
Vol 178 (3) ◽  
pp. 129-138 ◽  
Author(s):  
D. Nosi ◽  
M. Vassalli ◽  
L. Polidori ◽  
R. Giannini ◽  
A. Tani ◽  
...  
Keyword(s):  
Cell Contraction

A Phase Compensation Technique of Sampling Moiré Method for Accurate Single-Shot Phase Analysis

2011 ◽  
Vol 70 ◽  
pp. 243-248
Author(s):  
Shien Ri ◽  
Takashi Muramatsu ◽  
Masumi Saka
Keyword(s):  
Computer Simulation ◽  
Phase Analysis ◽  
Phase Error ◽  
Linear Interpolation ◽  
Small Displacement ◽  
Single Shot ◽  
Phase Compensation ◽  
Compensation Technique ◽  
Moire Method ◽  
Effective Phase

Recently, a technique for fast and accurate phase analysis called sampling moiré method has been developed for measurement of small-displacement distribution. In this study, a distribution of phase error caused by linear interpolation in case with mismatch between the sampling pitch and the grating pitch is theoretically analyzed. Moreover, a technique for effective phase compensation is proposed to reduce the periodic phase error. The performance of our compensation method is validated by a computer simulation. Phase analysis can be performed more accurately even in the case that the sampling pitch does not match to the grating pitch strictly.

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