In Ulcerative Colitis, The Damage Caused to Gut Cells Is Inflicted by the toxic Effects of Auto Immune Complexes, Neutrophils Nets and Pro Inflammatory Agents, Cationic Histones and Bacteriolyis Which Can All Be Strongly Modulated by Highly Anionic Heparins

Karwinskia humboldtiana is a poisonous plant that grows in semi desertic areas in north and central México. It produces several substances with different toxic effects. One of them designated T-514 damages severely the lung, kidney and liver, producing in the hepatoeyte large intracellular fat deposits and necrosis. Preliminary observations demonstrated that three is a decrease in the amount of peroxisomes in the hepatocytes of experimentally intoxicated rats and monkeys. To study the effect exerted by the T-514 on peroxisomes, a yeast model was selected, thus, three species: Saccha romices cerevisiae, Ilansenula polymorpha and Candida boidinii were used, because there is information concerning their peroxisome's morphology, enzyme content, biological behaviour under different culture conditions and biogenesis.

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Some Conditions Necessary for Pinocytosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100457 ◽

1968 ◽

Vol 26 ◽

pp. 142-143

Author(s):

M. W. Brightman

Keyword(s):

Smooth Muscle ◽

Cell Membrane ◽

Toxic Effects ◽

Cytological Evidence ◽

Cell Processes

The cytological evidence for pinocytosis is the focal infolding of the cell membrane to form surface pits that eventually pinch off and move into the cytoplasm. This activity, which can be inhibited by oxidative and glycolytic poisons, is performed only by cell processes that are at least 300A wide. However, the interpretation of such toxic effects becomes equivocal if the membrane invaginations do not normally lead to the formation of migratory vesicles, as in some endothelia and in smooth muscle. The present study is an attempt to set forth some conditions under which pinocytosis, as distinct from the mere inclusion of material in surface invaginations, can take place.

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A Sensitive On-Grid Immunoelectron Microscopic Procedure for Diagnosis of Rotavirus Infection

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s143192760000221x ◽

1980 ◽

Vol 38 ◽

pp. 506-507

Author(s):

M. F. Miller ◽

A. R. Rubenstein

Keyword(s):

Electron Microscopy ◽

Specific Antibody ◽

Immune Complexes ◽

Acute Gastroenteritis ◽

Plant Viruses ◽

Aggregation Process ◽

Microscopic Technique ◽

Fecal Material ◽

Present Communication ◽

Number Of Particles

Studies of rotavirus particles in humans, monkeys and various non-primates with acute gastroenteritis have involved detection of virus in fecal material by electron microscopy. The EM techniques most commonly employed have been the conventional negative staining (Fig. 1) and immune aggregation (Fig. 2) procedures. Both methods are somewhat insensitive and can most reliably be applied to samples containing large quantities of virus either naturaLly or as a result of concentration by ultracentrifugation. The formation of immune complexes by specific antibody in the immune aggregation procedures confirms the rotavirus diagnosis, but the number of particles per given microscope field is effectively reduced by the aggregation process. In the present communication, we describe use of an on-grid immunoelectron microscopic technique in which rotavirus particles are mounted onto microscope grids that were pre-coated with specific antibody. The technique is a modification of a method originalLy introduced by Derrick (1) for studies of plant viruses.

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Localization of Immune Complexes in the Basement Membrane of the Ductuli Efferentes of the Rhesus Monkey

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600001963 ◽

1980 ◽

Vol 38 ◽

pp. 456-457

Author(s):

D. Marsh

Keyword(s):

Basement Membrane ◽

Fluorescence Microscopy ◽

Rhesus Monkey ◽

Immune Complex ◽

Immune Complexes ◽

Vas Deferens ◽

Frozen Sections ◽

Efferent Ducts ◽

Complex Deposition ◽

Sperm Antibodies

As a result of vasectomy, spermatozoa are confined to the epididymis and vas deferens, where they degenerate, releasing antigens that enter the circulation or are engulfed by macrophages. Multiple antigens of the sperm can elicit production of autoantibodies; circulating anti-sperm antibodies are found in a large percentage of vasectomized men, indicating the immunogenicity of the sperm. The increased prevalence of macrophages in the liomen of the rhesus monkey testicular efferent ducts after vasectomy led to further study of this region. Frozen sections were used for evaluation of immunopathological status by fluorescence microscopy with fluorescein-conjugated antibody. Subsequent granular deposits of immune complexes were revealed by positive immunofluorescence staining for complement. The immune complex deposition in the basement membrane surrounding the efferent ducts implies that this region is involved in antigen leakage (Fig. 1).

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