scholarly journals Stress responses of Indian major carps cultured in the East Kolkata Wetland, West Bengal, India

2021 ◽  
Vol 4 (4) ◽  
pp. 351-362
Author(s):  
Anish Das ◽  
Talagunda Srinivasan Nagesh ◽  
Sarita Das ◽  
Thangapalam Jawahar Abraham
Keyword(s):  
Stress Responses ◽  
Total Protein ◽  
Serum Glucose ◽  
High Serum ◽  
Winter Temperature ◽  
Catla Catla ◽  
Cirrhinus Mrigala ◽  
East Kolkata Wetland ◽  
Indian Major Carps ◽  
Major Carps

Fish are continuously exposed to multiple environmental stressors that work cumulatively and synergistically. This study assessed the stress responses of Indian major carps (IMCs) cultured in a sewage-fed pond (SP) in the East Kolkata Wetland (EKW), India and compared with the normal carps in situ. The experiment was conducted in two farms that cultured Labeo rohita, Catla catla and Cirrhinus mrigala for seven months, covering the summer and winter periods. Serum biomarkers of primary (cortisol) and secondary (glucose, total protein, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH)) stress responses, and oxidative stress (superoxide dismutase (SOD)) were quantified using standard kits. The health status of carps was evaluated as a tertiary response. The biological oxygen demand, hardness, total dissolved solids, ammonia and phosphate levels of both ponds exhibited marked variations. The SP carps had significantly low haemoglobin and total protein, and high serum glucose, creatinine, ALT, AST and LDH levels. The SOD and cortisol levels were comparatively low in SP carps. The winter temperature had a significant effect on serum glucose, cortisol, SOD, creatinine, ALT and AST. Carps had a high degree of ectoparasitic infestation during the winter. Cirrhinus mrigala of the SP had significantly high serum creatinine levels. The increasing levels of serum glucose, creatinine, ALT and AST suggested that these indices, which were more pronounced in the carps of EKW in conjunction with winter temperature, could be useful biomarkers of stress, kidney and liver functioning in carps, respectively.

scholarly journals Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822)

2020 ◽  
Vol 67 (2) ◽  
Author(s):  
Snatashree Mohanty ◽  
M. Makesh ◽  
K. V. Rajendran ◽  
P. P. Suresh Babu ◽  
Deepika Anand ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cross Reactivity ◽  
Catla Catla ◽  
Cirrhinus Mrigala ◽  
Indian Major Carps ◽  
Sds Page ◽  
Igg1 Isotype ◽  
Major Carps

Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purified by affinity chromatography using BSA-CL agarose column. The purified mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purified m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purified m-Ig indicated that, all the three MAbs were specific to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps.

scholarly journals Suitability of Mahseer Tor putitora (Hamilton) in Polyculture with Indian Major Carps

2014 ◽  
Vol 18 (2) ◽  
pp. 175-182
Author(s):  
MR Rahman ◽  
MS Rahman ◽  
MGQ Khan ◽  
S Mostary
Keyword(s):  
Labeo Rohita ◽  
Stocking Density ◽  
The Body ◽  
Catla Catla ◽  
Cirrhinus Mrigala ◽  
Indian Major Carps ◽  
Significant Difference ◽  
Tor Putitora ◽  
Negative Effect ◽  
Major Carps

An experiment on polyculture of mahseer with Indian major carps at same stocking density was carried out for a period of 10 months from February 1 to December 1, 2004 in six experimental ponds. There were three treatments with two replicates each. Treatment 1 was designed with catla (Catla catla), rohu (Labeo rohita) and mrigal (Cirrhinus mrigala), treatment 2 with catla (Catla catla), rohu (Labeo rohita) and mahseer (Tor putitora) while treatment 3 with catla (Catla catla), rohu (Labeo rohita), mrigal (Cirrhinus mrigala) and mahseer (Tor putitora). Mahseer of the treatment 2 was introduced instead of mrigal of treatment 1 and 50% of mrigal was replaced with mahseer in treatment 3. The stocking density in all the treatments was 30 fish/decimal (7500 fish/ha). The feeds were supplied at the rate of 3-7% of the body weight of fish. The gross and net productions recorded were 5362.78 and 5188.64, 5431.42 and 5262.32, 5357.11 and 5528.74 kg/ha/yr in treatments 1, 2 and 3 respectively. Though there was no significant difference (p>0.05) among the three treatments using ANOVA but the production in treatment-3 was slightly higher than treatments 1 and treatment 2. However, there was no negative effect of mahseer on the growth and production of Indian major carps. So, it can be concluded that polyculture of mahseer with Indian major carps (catla, rohu and mrigal) can be done in ponds.DOI: http://dx.doi.org/10.3329/pa.v18i2.18194 Progress. Agric. 18(2): 175 - 182, 2007

Effects of Water Borne Iron on Spawn of Indian Major Carps (Catla catla (Ham.), Labeo rohita (Ham.) and Cirrhinus mrigala (Ham.))

2012 ◽  
Vol 89 (6) ◽  
pp. 1170-1174 ◽  
Author(s):  
Mitra Debnath ◽  
Ratan Kumar Saha ◽  
Dibyendu Kamilya ◽  
Dipangka Saikia ◽  
Himadri Saha
Keyword(s):  
Labeo Rohita ◽  
Catla Catla ◽  
Cirrhinus Mrigala ◽  
Indian Major Carps ◽  
Water Borne ◽  
Major Carps

scholarly journals The Relationship of Ova Diameter to Fertilization Rates, Hatching Rates, Survival Percentages and Specific Growth Rates in the Common Carp and Indian Major Carps

2007 ◽  
Vol 19 (3) ◽  
Author(s):  
R.K. SINGH ◽  
P.A. KHANDAGALE ◽  
S.L. CHAVAN ◽  
P.H. SAPKALE
Keyword(s):  
Growth Rate ◽  
Cyprinus Carpio ◽  
Specific Growth ◽  
Labeo Rohita ◽  
Fertilization Rate ◽  
Catla Catla ◽  
Cirrhinus Mrigala ◽  
Indian Major Carps ◽  
Relationship Of ◽  
Major Carps

The effects of different ova diameters on hatching, growth and survival of fry of the common carp (Cyprinus carpio var. communis, Cyprinidae) and Indian major carps viz.Catla catla, Labeo rohita, Cirrhinus mrigala (Cyprinidae) were examined. The four female brooders of different weight and size of each of Cyprinus carpio var. communis, Catla catla; Labeo rohita and Cirrhinus mrigala were collected. The highest mean ova diameter was observed in (Cyc 4) 1.235 ± 0.006 mm, (Cc 4) 5.386 ± 0.098 mm, (Lr 4) 4.94 ± 0.167 mm and (Cm 4) 4.95 ± 0.091 mm; highest fertilization rate (Cyc 4) 92.48%, (Cc 4) 85.42%, (Lr 4) 89.64%, (Cm 4) 86.49%; highest hatching percentage (Cyc 4) 82.32%, (Cc 4) 79.67%, (Lr 4) 81.28%, (Cm 4) 80.17% and highest survival (Cyc 4) 75.57%, (Cc 4) 76.36%, (Lr 4) 80.51%, (Cm 4) 68.35% respectively. The specific growth rate was also found highest in Cyprinus carpio var. communis (Cyc 4) 5.94%, Catla catla (Cc 4) 5.62%, Labeo rohita (Lr 4) 5.81% and Cirrhinus mrigala (Cm 4) 5.86% in fry of brooders having maximum weight. The direct relationship of ova diameter to fertilization rate, hatching, survival percentage and the specific growth rate of Cyprinus carpio var. communis, Catla catla, Labeo rohita and Cirrhinus mrigala were recorded. However, after the fifth week, the growth rate of spawn reduced in all the groups and was least at the end of the eighth week.

Sign in / Sign up

Export Citation Format

Share Document