scholarly journals A novel approach to trypsin inhibition by flavonoids

2021 ◽  
Vol 14 ◽  
Author(s):  
Alejandra I. Martinez-Gonzalez ◽  
Ángel G. Díaz-Sánchez ◽  
Laura A. De la Rosa ◽  
Ismael Bustos-Jaimes ◽  
Emilio Alvarez-Parrilla
Keyword(s):  
Diabetes Mellitus Type 2 ◽  
Mixed Type ◽  
Hydrophobic Interactions ◽  
Porcine Pancreas ◽  
Novel Approach ◽  
Obesity And Diabetes ◽  
Trypsin Inhibition ◽  
Type Mode ◽  
Amylase Inhibitors

Trypsin is a key protease related to digestion and absorption of proteins, which its inhibition must be studied when natural compounds, such as flavonoids, are used as part of alternative treatments for obesity and diabetes mellitus type 2, since trypsin and together with other pancreatic enzymes worked at small intestine. Considering that flavonoids are good lipase and amylase inhibitors, trypsin-flavonoids interactions were analyzed through UV-Vis, intrinsic and extrinsic fluorescence spectroscopies, circular dichroism, and molecular docking. The interaction between porcine pancreas trypsin and five flavonoids: hesperetin (HES), luteolin (LUT), quercetin (QUE), catechin (CAT), and rutin (RUT) was evaluated. Most of them exhibited a mixed-type mode. LUT was the best trypsin inhibitor (e.g., lower IC50, 45.20±1.00 µM). All flavonoids-trypsin complexes showed static quenching, and QUE and LUT exhibited higher affinity (higher Ka values, 0.90±0.10 and 1.60±0.20·10-1 mM-1, respectively). Hydrophobic interactions between trypsin and flavonoids were predominant.

scholarly journals The Beige Adipocyte as a Therapy for Metabolic Diseases

2019 ◽  
Vol 20 (20) ◽  
pp. 5058 ◽  
Author(s):  
Fernando Lizcano
Keyword(s):  
Diabetes Mellitus ◽  
Adipose Tissue ◽  
Diabetes Mellitus Type 2 ◽  
Metabolic Diseases ◽  
Brown Fat ◽  
Diabetes Mellitus Type ◽  
Beige Adipocyte ◽  
Obesity And Diabetes ◽  
Beige Adipocytes

Adipose tissue is traditionally categorized into white and brown relating to their function and morphology. The classical white adipose tissue builds up energy in the form of triglycerides and is useful for preventing fatigue during periods of low caloric intake and the brown adipose tissue more energetically active, with a greater number of mitochondria and energy production in the form of heat. Since adult humans possess significant amounts of active brown fat depots and its mass inversely correlates with adiposity, brown fat might play an important role in human obesity and energy homeostasis. New evidence suggests two types of thermogenic adipocytes with distinct developmental and anatomical features: classical brown adipocytes and beige adipocytes. Beige adipocyte has recently attracted special interest because of its ability to dissipate energy and the possible ability to differentiate themselves from white adipocytes. The presence of brown and beige adipocyte in human adults has acquired attention as a possible therapeutic intervention for metabolic diseases. Importantly, adult human brown appears to be mainly composed of beige-like adipocytes, making this cell type an attractive therapeutic target for obesity and obesity-related diseases, such as atherosclerosis, arterial hypertension and diabetes mellitus type 2. Because many epigenetics changes can affect beige adipocyte differentiation from adipose progenitor cells, the knowledge of the circumstances that affect the development of beige adipocyte cells may be important to new pathways in the treatment of metabolic diseases. New molecules have emerged as possible therapeutic targets, which through the impulse to develop beige adipocytes can be useful for clinical studies. In this review will discuss some recent observations arising from the unique physiological capacity of these cells and their possible role as ways to treat obesity and diabetes mellitus type 2.

scholarly journals The new views on the state of the gut microbiota in obesity and diabetes mellitus type 2

2019 ◽  
Vol 22 (3) ◽  
pp. 253-262
Author(s):  
Elena V. Pokrovskaya ◽  
Minara S. Shamkhalova ◽  
Marina V. Shestakova
Keyword(s):  
Diabetes Mellitus ◽  
Gut Microbiota ◽  
Human Body ◽  
Diabetes Mellitus Type 2 ◽  
Metabolic Disorders ◽  
Developed Countries ◽  
Diabetes Mellitus Type ◽  
Obesity And Diabetes

Obesity is a worldwide problem of the last century, the prevalence of which has reached pandemic proportions in developed countries. Over the past few years, a considerable amount of data has been gathered, reporting a direct link between changes in gut microbiota and the development of obesity, as well as related diseases, primarily, diabetes mellitus type 2. The elaboration of optimal methods of prevention and treatment regimens of these diseases needs to structure the existing knowledge about the mechanisms of development of metabolic disorders, the role of intestinal microbiota in the latter and possible therapeutic targets. This review examines the role of microorganisms in the human body, with the main focus on the developmental origins of metabolic disorders using animal models and accumulated experience of research on their effects on the human body, and also discusses possible treatment options, including bariatric surgery, fecal microbiota transplantation, the use of pre- and probiotics and certain particular groups of glucose-lowering drugs.

scholarly journals Effect of obesity and diabetes mellitus type 2 on vascular stiffness

2015 ◽  
Author(s):  
Olga Bourko ◽  
Tatiana Netchessova ◽  
Irina Bourko ◽  
Larisa Danilova ◽  
Elena Dashkevich ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetes Mellitus Type 2 ◽  
Vascular Stiffness ◽  
Diabetes Mellitus Type ◽  
Obesity And Diabetes

The protective roles of clusterin in ocular diseases caused by obesity and diabetes mellitus type 2

2021 ◽  
Author(s):  
Thaís Dantis Pereira de Campos ◽  
Kellen Cristina da Cruz Rodrigues ◽  
Rodrigo Martins Pereira ◽  
Chadi Pellegrini Anaruma ◽  
Raphael dos Santos Canciglieri ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetes Mellitus Type 2 ◽  
Diabetes Mellitus Type ◽  
Ocular Diseases ◽  
Obesity And Diabetes

scholarly journals Lipid-proteinic interaction in erythrocytie and placental membranes in women with insulin resistance

2012 ◽  
Vol 58 (2) ◽  
pp. 224-229
Author(s):  
N.P. Mikaelyan ◽  
A.A. Terentyev ◽  
A.G. Maxina ◽  
A.V. Mikaelyan ◽  
S.V. Novikova
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Resistance ◽  
Pregnant Women ◽  
Diabetes Mellitus Type 2 ◽  
Diabetes Mellitus Type ◽  
Erythrocyte Membranes ◽  
Obesity And Diabetes ◽  
Placental Membranes ◽  
Lipid Spectrum

Disturbances of erythrocyte and placental membrane functiond have been studied in placenta of pregnant women with obesity and diabetes mellitus type 2. The results of this study demonstrate significant metabolic impairments in women with insulin resistance. Changes in lipid spectrum of erythrocyte membranes and decreased activity of antioxidant enzymes obviously contribute to the development of fetoplacental insufficiency. This changes point to necessity of the antioxidant therapy in pregnant women with obesity and diabetes mellitus type 2.

scholarly journals Corrigendum: The new views on the state of the gut microbiota in obesity and diabetes mellitus type 2 (Diabetes mellitus. 2019;22(3). doi: 10.14341/DM10194)

2019 ◽  
Vol 22 (4) ◽  
pp. 403-404
Author(s):  
Elena V. Pokrovskaya ◽  
Minara S. Shamkhalova ◽  
Marina V. Shestakova
Keyword(s):  
Diabetes Mellitus ◽  
Gut Microbiota ◽  
Inflammatory Cytokines ◽  
Diabetes Mellitus Type 2 ◽  
Interleukin 1 ◽  
The State ◽  
Diabetes Mellitus Type ◽  
Obesity And Diabetes ◽  
Anti Inflammatory

A corrigendum on The new views on the state of the gut microbiota in obesity and diabetes mellitus type 2 by Elena V. Pokrovskaya, Minara S. Shamkhalova, Marina V. Shestakova (2019). Diabetes Mellitus. 22(3). doi: 10.14341/DM10194There is an error on the page 255: "Moreover, in obese patients, the concentration of circulating LPS increases by 20%, and in patients with diabetes mellitus, it increases by 125%. LPS is transported from cells of the large intestine into the bloodstream through chylomicrons or through intercellular gaps in the intestinal wall; by forming a complex of CD14 with Toll-like receptor 4 of macrophages and endothelial cells, it causes the release of anti-inflammatory cytokines: namely, interleukin-1, interleukin-6 and tumour necrosis factor alpha ". Instead of " pro-inflammatory cytokines" was published " anti-inflammatory cytokines ".Literary source Dahiya DK, Renuka, Puniya M, et al. Gut Microbiota Modulation and Its Relationship with Obesity Using Prebiotic Fibers and Probiotics: A Review. Front Microbiol. 2017;8:563. doi: https://doi.org/10.3389/fmicb.2017.00563 is listed twice (№№ 7 and 13) in the list of references.The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.The original article has been updated.

Sign in / Sign up

Export Citation Format

Share Document