scholarly journals A single nucleotide polymorphism in the acetyl-coenzyme A acyltransferase 2 (ACAA2) gene is associated with milk yield in Chios sheep

2012 ◽  
Vol 95 (6) ◽  
pp. 3419-3427 ◽  
Author(s):  
M. Orford ◽  
G. Hadjipavlou ◽  
O. Tzamaloukas ◽  
D. Chatziplis ◽  
A. Koumas ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Milk Yield ◽  
Coenzyme A ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme ◽  
Chios Sheep

scholarly journals DGAT1 GENE POLYMORPHISM AND ITS RELATIONSHIPS WITH CATTLE MILK YIELD AND CHEMICAL COMPOSITION

2020 ◽  
Vol 17 (35) ◽  
pp. 174-180
Author(s):  
Salah H. FARAJ ◽  
Asaad Y. AYIED ◽  
D. K. SEGER
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Chemical Composition ◽  
Milk Yield ◽  
Haplotype Diversity ◽  
Genotypic Frequency ◽  
Production Traits ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Dgat1 Gene ◽  
Fat Yield

Several polymorphisms in different gene loci have been noted to affect production traits such as milk yield and milk composition. The present study aimed to determine the allelic and genotypic frequency of the DGAT1 gene and evaluate the associations between DGAT1 genetic variants and milk yield and its chemical composition of local Iraqi cattle. Blood samples from 100 cows were obtained for DNA isolation. The primer used in this study amplified 411-bp fragments at exon 8 of the DGAT1 gene. DNA sequencing methods were applied to detect single nucleotide polymorphism of the DGAT1 gene in 100 cows. The nucleotide sequences of exon 8 of the DGAT1 gene were registered for local Iraqi cattle in the National Center for Biotechnology Information (NCBI), DNA Data Bank of Japan (DDBJ), and the European Nucleotide Archive (ENA) under the following accession numbers (LC492073 and LC492074). The results showed the presence of two polymorphic sites leading to the construction of 2 different haplotypes in the cow. Haplotype diversity was 0.536, while nucleotide diversity was 0.0031. Two single-nucleotide polymorphism (SNP) loci of the DGAT1 gene were detected, namely A10433G (A/G) and A10434C (A/C). The resulting of this mutation changes lysine to alanine substitution at position 232 (A232K mutation) of amino acid sequence. Geneious software V. 2020.0.4 was used to detect genotypes of the DGAT1 gene, as the sequence alignment showed the presence of three genotypes. The genotypic frequencies of KK, KA, and AA were 0.40, 0.30, and 0.30, respectively. Frequencies of K and A alleles were 0.60 and 0.40, respectively. The KK genotype was significantly (P 0.05) associated with higher fat yield. Therefore, the DGAT1 gene could serve as a genetic marker for the selection of fat yield in cows.

scholarly journals Effect of Single Nucleotide Polymorphism rs1044925 in Acyl-CoA: Cholesterol Acyltransferase-1 Gene on Plasma Lipid Parameters in Patients with Ischemic Stroke

2021 ◽  
pp. 41-52
Author(s):  
Johnson Oshiobugie Momoh
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Ischemic Stroke ◽  
Coenzyme A ◽  
Cholesterol Acyltransferase ◽  
Plasma Lipid ◽  
Lipid Profiles ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Acyl Coenzyme A ◽  
Lipid Parameters

Acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) catalyzes the synthesis of cholesteryl esters from cholesterol and fatty acyl-CoA in tissues and the enzyme plays a major role in atherosclerosis and cellular cholesterol homeostasis. The study shows the effect of single nucleotide polymorphism rs1044925 in acyl-CoA:cholesterol acyltransferase-1 gene on plasma lipid parameters in patients with ischemic stroke. 100 patients with ischemic stroke and 100 controls matched for sex and aged 46-87 were selected for the study. Lipid profiles were measured using Randox kits and lipoprotein ratios were calculated using Excel software. The genotyping of the acyl-coenzyme A: cholesterol acyltransferase-1 rs1044925 SNP were performed by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) combined with 2% gel electrophoresis. There were significant difference (P<0.05) in the genotypic and allelic frequencies of ACAT-1 rs1044925 SNP between the normolipidemic and patients with ischemic stroke. The frequencies of AC, CC and AA genotypes of the ACAT-1 gene were 33%, 3% and 64% for the control and 57%, 5% and 38%, for the stroke subjects respectively. The frequencies of C and A alleles were 19.50% and 80.50% for the control and 33.50% and 66.50% for the ischemic stroke subjects (P < 0.0001) respectively. The effects of genotypes on plasma lipid profiles and lipoprotein ratios were found for both control and stroke subjects. The A allele carriers of ACAT-1 rs1044925 SNP had lower plasma TG, TC, VLDL-C and other lipid parameters as compared to the C allele carriers for both subjects. The C allele carriers were responsible for the increase in dyslipidemia for both subjects. The results of this study show that the polymorphism of rs1044925 in the ACAT-1 gene as effect on plasma lipid profiles and lipoprotein ratios in ischemic stroke patients.

Association of single nucleotide polymorphism of Hsp90ab1 gene with thermotolerance and milk yield in Sahiwal cows

2015 ◽  
Vol 9 (8) ◽  
pp. 99-103 ◽  
Author(s):  
Sailo Lalrengpuii ◽  
D Gupta I ◽  
Verma Archana ◽  
Das Ramendra ◽  
V Chaudhari M
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Milk Yield ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Sahiwal Cows

Identification of a single nucleotide polymorphism at intron 16 of the caprine Acyl-Coenzyme A: diacylglycerol acyltransferase 1 (DGAT1) gene

2006 ◽  
Vol 74 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Antonella Angiolillo ◽  
Marcel Amills ◽  
Baltasar Urrutia ◽  
Anna Doménech ◽  
Yolanda Sastre ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Milk Fat ◽  
Coenzyme A ◽  
Association Studies ◽  
Fatty Acyl ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Triacylglycerol Synthesis ◽  
Exon 1 ◽  
Acyl Coenzyme A

The DGAT1 gene encodes a microsomal enzyme that catalyses the only committed step in triacylglycerol synthesis by joining diacylglycerol and fatty acyl coenzyme A. In cattle, a K232A substitution in the DGAT1 molecule has a significant effect on enzyme activity and milk fat content. The prominent role of this gene in lipid metabolism led us to undertake the structural characterization of DGAT1 in goats. In this way, we have sequenced a 1552 bp fragment of the goat DGAT1 cDNA, which encompasses most of the coding sequence (from exon 1 to 17), and a genomic fragment covering exons 12 to 17. Multiple alignment of the goat DGAT1 sequences revealed the existence of a single nucleotide polymorphism (SNP) involving a T to C substitution at intron 16. We optimized a primer extension based genotyping method that allowed us to determine that the C variant is a minority allele with frequencies ranging from 0·062 (Murciano-Granadina) to 0·109 (Malagueña). This SNP, although not expected to have any functional effect, might be useful as a genetic marker in association studies to detect additional DGAT1 polymorphisms which might influence fat milk content and other traits of economic interest.

scholarly journals A single nucleotide polymorphism in the 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene (HMGCR) influences the serum triacylglycerol relationship with dietary fat and fibre in the European Prospective Investigation into Cancer and Nutrition in Norfolk (EPIC-Norfolk) study

2010 ◽  
Vol 104 (5) ◽  
pp. 765-772 ◽  
Author(s):  
Renata N. Freitas ◽  
Kay-Tee Khaw ◽  
Kelvin Wu ◽  
Richard Bowman ◽  
Hannah Jeffery ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Dietary Fat ◽  
Serum Lipids ◽  
Coenzyme A ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Fibre Intake ◽  
Reductase Gene ◽  
Control Serum ◽  
Coenzyme A Reductase

The objective of the present study was to investigate the influence of the single nucleotide polymorphism (rs17238540) at the 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene (HMGCR) on the relationship between serum lipids and dietary fat and fibre (NSP). FFQ and pyrosequencing were used to assess cross-sectional dietary intake andHMGCRgenotype in a population study with data for serum lipids available. Genotype frequencies and allele distributions for 23 011 participants were: TT 95·65 %, TG 4·29 % and GG 0·06 %; T 97·8 % and G 2·2 %. In regression analyses, the TG+GG group showed a significant positive relationship between TAG and SFA intake (+0·11 (95 % CI 0·02, 0·20) mmol TAG/l;P = 0·017; per 3 % SFA energy increase) while the TT individuals showed no change in the TAG levels related to SFA intake ( − 0·0007 (95 % CI − 0·02, 0·02) mmol TAG/l;P = 0·99). TG+GG individuals showed an inverse relationship between TAG and fibre intake higher ( − 0·14 (95 % CI − 0·22, − 0·05) mmol TAG/l than the TT group ( − 0·04 (95 % CI − 0·06, − 0·02) mmol TAG/l). In both cases the respective coefficient regressions of TAG were different between the genotype groups (Z = 2·27,P = 0·023 for SFA intake; Z = 2·19,P = 0·029 for fibre intake). Individuals carrying the G allele may show a greater response in lower TAG levels with reduced SFA intake and increased fibre intake compared with those homozygous for the T allele. The effectiveness of different dietary interventions to control serum lipids may vary according toHMGCRgenotype.

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