Occurrence of methicillin-resistant Staphylococcus aureus in dairy cattle herds, related swine farms, and humans in contact with herds

Methicillin-resistant Staphylococcus aureus (MRSA) harboring the type-IX staphylococcal cassette chromosome mec (SCCmec) has been found in pigs and humans in Northern Thailand. However, knowledge of the prevalence and acquisition risk factors of this MRSA strain among swine production personnel (SPP) are needed. The nasal swab samples and data were collected from 202 voluntary SPP and 31 swine farms in Chiang Mai and Lamphun Provinces, Thailand in 2017. MRSA were screened and identified using mannitol salt agar, biochemical and antimicrobial susceptibility testing, multiplex PCR, and the SCCmec typing. The prevalence of MRSA was 7.9% (16/202) and 19.3% (6/31) among SPP and swine farms. All isolates were multidrug-resistant, and 55 of 59 isolates (93%) contained the type-IX SCCmec element. Data analysis indicated that education, working time, contact frequency, working solely with swine production, and personal hygiene were significantly related to MRSA acquisition (p < 0.05). The multivariate analysis revealed that pig farming experience, working days, and showering were good predictors for MRSA carriage among SPP (area under the curve (AUC) = 0.84). The biosecurity protocols and tetracycline use were significantly associated with MRSA detection in pig farms (p < 0.05). Hence, the active surveillance of MRSA and further development of local/national intervention for MRSA control are essential.

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Prevalence and Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) Mastitis in Dairy Cattle in Jabalpur, Madhya Pradesh

Journal of Animal Research ◽

10.5958/2277-940x.2017.00011.0 ◽

2017 ◽

Vol 7 (1) ◽

pp. 77

Author(s):

Neeraj Shrivastava ◽

Varsha Sharma ◽

Anju Nayak ◽

A.B. Shrivastava ◽

B.C. Sarkhel ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Dairy Cattle ◽

Methicillin Resistant Staphylococcus Aureus ◽

Madhya Pradesh ◽

Methicillin Resistant

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Methicillin-Resistant Staphylococcus aureus in Pigs and Farm Workers on Conventional and Antibiotic-Free Swine Farms in the USA

PLoS ONE ◽

10.1371/journal.pone.0063704 ◽

2013 ◽

Vol 8 (5) ◽

pp. e63704 ◽

Cited By ~ 70

Author(s):

Tara C. Smith ◽

Wondwossen A. Gebreyes ◽

Melanie J. Abley ◽

Abby L. Harper ◽

Brett M. Forshey ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Farm Workers ◽

Methicillin Resistant ◽

Swine Farms ◽

The Usa

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The first outbreak of methicillin-resistant Staphylococcus aureus in dairy cattle in Poland with evidence of on-farm and intrahousehold transmission

Journal of Dairy Science ◽

10.3168/jds.2020-18291 ◽

2020 ◽

Vol 103 (11) ◽

pp. 10577-10584

Author(s):

Henryk Krukowski ◽

Zofia Bakuła ◽

Mateusz Iskra ◽

Alina Olender ◽

Hanna Bis-Wencel ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Dairy Cattle ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistant ◽

On Farm

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Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus clonal complexes in bulk tank milk from dairy cattle herds in Lombardy Region (Northern Italy)

Epidemiology and Infection ◽

10.1017/s0950268816001576 ◽

2016 ◽

Vol 144 (14) ◽

pp. 3046-3051 ◽

Cited By ~ 21

Author(s):

C. CORTIMIGLIA ◽

M. LUINI ◽

V. BIANCHINI ◽

L. MARZAGALLI ◽

F. VEZZOLI ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Dairy Cattle ◽

Northern Italy ◽

Bulk Tank Milk ◽

Methicillin Resistant ◽

Lombardy Region ◽

Single Locus Variant ◽

Bulk Tank ◽

Cattle Herds ◽

Mrsa Strains

SUMMARYStaphylococcus aureus is the most important causative agent of subclinical mastitis in cattle resulting in reduced milk production and quality. Methicillin-resistant S. aureus (MRSA) strains has a clear zoonotic relevance, especially in the case of occupational exposure. The aim of the study was to evaluate the prevalence of S. aureus and MRSA in bulk tank milk (BTM) from dairy cattle herds in the Lombardy Region (Northern Italy) and to identify the main MRSA circulating genotypes. MRSA strains were characterized by susceptibility testing, multi-locus sequence typing (MLST), spa typing and SCCmec typing. A total 844 BTM samples were analysed and S. aureus and MRSA were detected in 47·2% and 3·8% of dairy herds, respectively. MLST showed that the majority (28/32) of isolates belonged to the typical livestock-associated lineages: ST398, ST97 and ST1. Interestingly, in this study we report for the first time the new ST3211, a single locus variant of ST(CC)22, with the newly described 462 aroE allele. Our study indicates high diffusion of S. aureus mastitis and low, but not negligible, prevalence of MRSA in the considered area, suggesting the need for planning specific control programmes for bovine mastitis caused by S. aureus, especially when MRSA is implicated.

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Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) in Cattle Milk from dairy Herds in Oyo State, Nigeria

Journal of Veterinary and Biomedical Sciences ◽

10.36108/jvbs/9102.20.0280 ◽

2020 ◽

Vol 2 (2) ◽

Author(s):

O. O Oludairo

Keyword(s):

Staphylococcus Aureus ◽

Dairy Cattle ◽

Methicillin Resistant Staphylococcus Aureus ◽

Dairy Herd ◽

Diffusion Method ◽

Herd Health ◽

Biochemical Tests ◽

Disk Diffusion Method ◽

Methicillin Resistant ◽

Milk Samples

Methicillin-resistant Staphylococcus aureus (MRSA) has received a lot of attention in recent years as a zoonotic organism of global concern. Contaminated milk, especially those from mastitic cows, serve as reservoirs for humans in the epidemiology of antibiotic resistant MRSA. This study was designed to determine the level of contamination of bulk fresh milk from dairy cattle herds with MRSA in Ibarapa, Oyo and Oke-Ogun areas of Oyo State and the antibiotic resistance profile of the isolates. One hundred and sixty-five (165) milk samples were obtained from the study areas and used for the study. Staphylococcus aureus was isolated from the samples using bacterial culture and biochemical tests. Methicillin-resistant Staphylococcus aureus was identified using cefoxitin disk diffusion method. All the S. aureus isolates were subjected to microbial susceptibility test. Ninety (54.5%) milk samples were positive for Staphylococcus spp. out of which 52 (31.5%) were Staphylococcus aureus and 13 (7.9%) yielded MRSA. Antibiogram of S. aureus indicated highest resistance to Cloxacillin (88.5%) followed by (Augmentin 67.3%) and Ceftrazidine (67.3%). Ten out of the 13 MRSA isolates were multidrug resistance while all the isolates were 100% susceptible to ofloxacin. The results of this study showed that milk produced from dairy cattle in Oyo State was contaminated with MRSA. This portends serious food safety and public health risk among the consumers of such milk especially in raw or improperly pasteurized form. Proper dairy herd health management and prudent use of antibiotics and hygienic milking procedures are hereby recommended to prevent contamination of milk and subsequent spread of the organism to humans.

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Prevalence of methicillin-resistant Staphylococcus aureus carrying mecA or mecC in dairy cattle

Veterinary Microbiology ◽

10.1016/j.vetmic.2013.12.024 ◽

2014 ◽

Vol 171 (3-4) ◽

pp. 364-367 ◽

Cited By ~ 16

Author(s):

E. van Duijkeren ◽

P.D. Hengeveld ◽

M. Albers ◽

G. Pluister ◽

P. Jacobs ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Dairy Cattle ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistant

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First mecC and mecA Positive Livestock-Associated Methicillin Resistant Staphylococcus aureus (mecC MRSA/LA-MRSA) from Dairy Cattle in Malaysia

Microorganisms ◽

10.3390/microorganisms8020147 ◽

2020 ◽

Vol 8 (2) ◽

pp. 147 ◽

Cited By ~ 5

Author(s):

Erkihun Aklilu ◽

Hui Ying Chia

Keyword(s):

Staphylococcus Aureus ◽

Dairy Cattle ◽

Methicillin Resistant Staphylococcus Aureus ◽

Meca Gene ◽

Pcr Amplification ◽

Diffusion Method ◽

Methicillin Resistant ◽

Unpasteurized Milk ◽

Antimicrobial Susceptibility Tests ◽

Pcr Targeting

Livestock associated Methicillin resistant Staphylococcus aureus (S. aureus) (LA-MRSA) was reported to be zoonotic and may transmit to farmers and veterinarians. The objectives of this study were to investigate the occurrence of LA-MRSA from dairy cattle and to evaluate the antimicrobial resistance profiles of the isolates. A total of 63 milk and 32 nasal swab samples were randomly collected from dairy cattle. The samples were processed to isolate S. aureus, MRSA and LA-MRSA using both phenotypic and molecular methods using PCR. The confirmed S. aureus isolates were cultured on oxacillin resistant screening agar base (ORSAB) to detect MRSA and the isolates were further confirmed by PCR targeting the mecA gene. Detection of the novel mecA gene, mecC gene was conducted by PCR amplification. The antimicrobial susceptibility tests were conducted using disc diffusion method. Results revealed 17/95 (17.89%) and 15/95 (15.79%) were positive for mecA and mecC genes respectively. Out of the 15 mecC positive isolates, 12 were positive for both mecA and mecC. The MRSA isolates showed multidrug resistance. The findings showed high prevalence of mecC-positive LA-MRSA in Malaysia and highlight the public health risks to people that may come in contact with the carrier animals or those who may consume unpasteurized milk products from these animals.

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