scholarly journals INFLUENCE OF STAT-3 TRANSCRIPTION FACTOR INHIBITOR ON OXIDATIVE AND NITROSATIVE STRESS PARAMETERS IN PERIODONTAL TISSUES OF RATS EXPOSED TO SYSTEMIC LIPOPOLISACCHARIDE ADMINISTRATION

2018 ◽  
Vol 18 (4) ◽  
pp. 102-106
Author(s):  
A.M. Yelins’ka ◽  
V.O. Kostenko
Keyword(s):  
Superoxide Dismutase ◽  
Transcription Factor ◽  
Imatinib Mesylate ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Nitrosative Stress ◽  
Total Activity ◽  
No Synthase ◽  
Oxidative And Nitrosative Stress ◽  
Periodontal Tissues

This study is aimed at investigating the effect of imatinib mesylate, an inhibitor of the transcription factor STAT-3, on the oxidative and nitrosative stress indicators in rat periodontal tissues during the experimental systemic inflammatory response (SIR) induced by the introduction of the Salmonella typhi lipopolysaccharide (LPS) (in a dose of 0.4 μg/kg body wt, 3 times for the 1 week and once a week through the next 7 weeks). Imatinib mesylate introduction in a dose of 15 mg/kg 3 times a week, starting from the 30th day of the SIR modeling, was accompanied by a significant decrease in the rate of production of superoxide anion radical by the mitochondrial respiratory chain (by 13.4%) compared with the data from the SIR modeled group. The production rate of this radical by NADPH-dependent electron transport chains and phagocytes did not change significantly. At the same time, in the periodontal tissues, the total activity of NO synthase decreased (by 27.4%) without significant changes in the concentration of peroxynitrite ions. As a result, lipid peroxidation (LPO) in periodontal soft tissues was limited: the concentration of secondary peroxidation products before and after the incubation in a prooxidant buffer solution when imatinib mesylate was added was inferior to the results of the SIR modeled group by 37.5 and 33.8%, respectively. The activity of superoxide dismutase and catalase exceeded the data of the comparison group by 40.0 and 60.0%, respectively. It was concluded that the use of the inhibitor of STAT-3 activation, imatinib mesylate, under LPS-induced SIR, limits the formation of reactive oxygen and nitrogen species in rat periodontal tissues: it decreases the production rate of superoxide anion-radical by the mitochondrial electron transport chain, reduces the total activity of NO synthase. This results in the reduced formation of secondary LPO products in periodontal tissues and the reduced activity of antioxidant enzymes in them (superoxide dismutase, catalase).

Correction of oxidative stress in patients with chronic cerebral ischemia

2016 ◽  
Vol 94 (7) ◽  
pp. 549-553
Author(s):  
Olga A. Goroshko ◽  
K. N. Novikov ◽  
V. G. Kukes ◽  
V. L. Voeikov ◽  
V. V. Arkhipov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Cerebral Ischemia ◽  
Pilot Study ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Feedback Mechanism ◽  
Chronic Cerebral Ischemia ◽  
Peroxidation Product ◽  
Reasonable Use

A pilot study ofthe effect ofthe antioxidant drug ethylmethylhydroxypyridine malate on indicators of oxidative stress in patients with chronic cerebral ischemia. At 6 day course administration investigated the antioxidant in these patients significantly accelerates the process of generation of superoxide anion radical, established by lucigenin-depended chemiluminescence that probably regulate a feedback mechanism oxidase systems. This increases the activity of superoxide dismutase, and reduced the concentration of secondary peroxidation product - malondialdehyde, making reasonable use of antioxidants in the treatment of this pathology.

Changes in the superoxide radical and superoxide dismutase levels in the uterus of Rattus norvegicus during the estrous cycle and a possible role for superoxide radical in uterine oedema and cell proliferation at proestrus

1991 ◽  
Vol 69 (4) ◽  
pp. 313-316 ◽  
Author(s):  
M. Laloraya ◽  
G. P. Kumar ◽  
M. M. Laloraya
Keyword(s):  
Cell Proliferation ◽  
Superoxide Dismutase ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Radical ◽  
Inverse Correlation ◽  
Oestrous Cycle ◽  
Superoxide Anion Radical ◽  
Albino Rat ◽  
Cyclic Changes

Superoxide anion radical, a radical whose toxicity is well documented, is present in the uterus of albino rat. The cyclic changes that it undergoes during the reproductive cycle are also illustrated. An inverse correlation is seen between the levels of superoxide radical and the enzyme dismutating it, i.e., superoxide dismutase (EC 1.15.1.1), which is also reported to be present in the uterus of albino rat and is shown to exhibit cyclic changes during its oestrous cycle. The high levels of superoxide radical at pro-oestrus enable us to hypothesize that superoxide may be involved in regulating the oedema and cell proliferation of the uterus during proestrus.Key words: superoxide anion radical, superoxide dismutase, uterus, oestrous cycle, uterine oedema, cell proliferation.

scholarly journals QUERCETIN LIMITS THE PROGRESSION OF OXIDATIVE AND NITROSATIVE STRESS IN THE RATS’ TISSUES AFTER EXPERIMENTAL TRAUMATIC BRAIN INJURY

2020 ◽  
Vol 73 (10) ◽  
pp. 2127-2132
Author(s):  
Ivan V. Yavtushenko ◽  
Svitlana M. Nazarenko ◽  
Oleksandr V. Katrushov ◽  
Vitalii O. Kostenko
Keyword(s):  
Cerebral Hemisphere ◽  
Nitrosative Stress ◽  
Water Soluble ◽  
Cerebral Hemispheres ◽  
Soluble Form ◽  
Buffer Solution ◽  
Experimental Conditions ◽  
Oxidative And Nitrosative Stress ◽  
Periodontal Tissues ◽  
White Rats

The aim: To investigate the effect of water-soluble form of quercetin on the indices reflecting the progression of oxidative-nitrosative stress in the cerebral tissues and the periodontium of rats after experimental TBI. Materials and methods: The studies were conducted on 30 white rats of the Wistar line weighing 180-220 g, divided into 3 groups: the 1st group included pseudo-injured animals (subjected to ether anaesthesia, fixation without TBI modeling), the 2nd group included the animals exposed to modeled moderate TBI, the 3rd group involved the rats, which were given injections with water-soluble form of quercetin (corvitin, “Borshchahivskiy CPP”, Ukraine) intraperitoneally in a daily dose of 10 mg/kg recalculated for quercetin for 7 days following the TBI modeling. The formation of superoxide radical anion (.О2 -), activity of NO-synthase – total (NOS), its constitutive and inducible isoforms (cNOS, iNOS) – and concentration of peroxynitrite were evaluated spectrophotometrically. The level of lipid peroxidation (LPO) in the tissues was evaluated by the formationof a stained trimethine complex during the reaction of tiobarbituric acid (TBA). The activity of the antioxidant system was assessed by increasing in the concentration of TBA active products during 1.5 hour incubation in iron-ascorbate buffer solution, as well as by the activity of antioxidant enzymes – superoxide dismutase (SOD) and catalase. Results: The use of quercetin under the experimental conditions significantly reduced the О2 - generation by NADPH- and NADH-dependent electron transport chains by 30.2 and 35.0% (in the cerebral hemispheres) and by 23.5 and 32.5% (in the soft periodontal tissues), respectively, compared to the findings in the 2nd group. The production of this radical by leukocyte NADPH oxidase in these organs was inferior to the value of the 2nd group by 39.3 and 29.9%. We revealed that the use of quercetin in the experimental conditions probably reduced the activity of NOS, including iNOS, by 38.2 and 45.3% (in the cerebral hemispheres) and by 53.5 and 66.9% (in the soft periodontal tissues), respectively, compared with the findings in the 2nd group. Under these conditions, the cNOS activity went up by 50.0% and doubled, the peroxynitrite content was lower by 19.5 and 32.1% than that in the 2nd group. The administration of quercetin in the experimental conditions significantly reduced the concentration of TBA-active products in the homogenate of cerebral hemispheres and soft periodontal tissues. The development of decompensated LPO is also confirmed by a decrease in the activity of SOD and catalase. Conclusions: on the 7th day after modeling moderate TBI in rats the signs of oxidative-nitrosative stress are found not only in locus morbi (in the tissue of the cerebral hemisphere), but also in distant organs (periodontal tissues). Applying of water-soluble form of quercetin significantly reduces signs of oxidative-nitrosative stress in the tissue of the cerebral hemisphere of rats, as well as in periodontal tissues on the 7th day after moderate TBI modeling.

scholarly journals QUERCETIN LIMITS THE DEVELOPMENT OF OXIDATIVE-NITROSATIVE STRESS IN PERIODONTAL TISSUES IN DIFFERENT MODELS SIMULATING SYSTEMIC INFLAMMATORY RESPONSE

2019 ◽  
Vol 19 (4) ◽  
pp. 83-87
Author(s):  
A.M. Yelins’ka ◽  
S.M. Nazarenko ◽  
V.O. Kostenko
Keyword(s):  
Inflammatory Response ◽  
Nadph Oxidase ◽  
Nitrosative Stress ◽  
Soft Tissues ◽  
Salmonella Typhi ◽  
No Synthase ◽  
Systemic Inflammatory Response ◽  
Water Soluble ◽  
Soluble Form ◽  
Periodontal Tissues

The study was carried out to investigate the effect produced by water-soluble form of quercetin (corvitin) on the indices of the development of oxidative-nitrosative stress in periodontal soft tissues of rats subjected to systemic inflammatory response. This condition was simulated by using two models: one was induced by the Salmonella typhi lipopolysaccharide (LPS) administration (in a dose of 0.4 μg / kg of body wt three times through the 1st week and once a week for the next 7 weeks), as well as on the 14th day after a moderate craniocerebral injury (CCI). Applying corvitin in a dose of 500 μg / kg (10 μg / kg recalculated as quercetin) every third day starting from the 30th day of the experiment with the use of pyrogenalum reduced the production of superoxide anion radical (.О) by NADPH-dependent electron transport chains (endoplasmic reticulum and NO-synthase) by 18.1%, but did not considerably affect the mitochondrial chain. .О production by leukocyte NADPH-oxidase was by 16.7% lower. The total NO synthase (NOS) activity in periodontal tissues decreased by 40.6%, and the content of peroxynitrite ions was inferior to the relevant result of the group received pyrogenalum by 13.9%. Administration of corvitin in a dose of 500 mg/kg for 7 days after CCI modeling reduced the (.О) production by NADPH-dependent chains by 20.9%, and by mitochondria by 31.7% on the 14th day of post-traumatic period. .О production by leukocyte NADPH-oxidase was by 35.8% lower. NOS activity in periodontal tissues decreased by 45.8%, the content of peroxynitrite ions was inferior to the relevant value in the group with modeled CCI by 25.7%. This suggests the conclusion that applying water-soluble form of quercetin in conditions of systemic inflammatory response limits the signs of oxidative-nitrosative stress in periodontal soft tissues of rats.

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