Koliko znamo o američkom cvrčku (Scaphoideus titanus Ball, 1932)?

Ankica Sarajlić; Emilija Raspudić; Ivana Majić; Toni Kujundžić; Mato Drenjančević

doi:10.31727/gzb.44.5.12

Koliko znamo o američkom cvrčku (Scaphoideus titanus Ball, 1932)?

Glasnik zaštite bilja ◽

10.31727/gzb.44.5.12 ◽

2021 ◽

Vol 44 (5) ◽

pp. 93-99

Author(s):

Ankica Sarajlić ◽

Emilija Raspudić ◽

Ivana Majić ◽

Toni Kujundžić ◽

Mato Drenjančević

Keyword(s):

Scaphoideus Titanus

Američki cvrčak (Scaphoideus titanus Ball, 1932) jedan je od najznačajnijih vektora zlatne žutice vinove loze. Zakonskim regulativama pokušava se vektora staviti pod nadzor, a poljoprivredni proizvođači dužni su provoditi mjere kako bi spriječili epidemijsko širenje bolesti. Prvi stadiji ličinke jako su sitni što proizvođačima otežava njihovo rano otkrivanje i određivanje roka za zaštitu od ovog štetnika. Kako bi pravovremeno primijenili zaštitu potrebno je pratiti pojavu štetnika jer njegov razvoj najviše ovisi o vremenskim uvjetima. Za suzbijanje američkog cvrčka uglavnom se primjenjuje kemijska zaštita. Jako je bitno paziti i na ostali biljni sastav u vinogradu te izbjeći druge biljke domaćine koje mogu poslužiti štetniku za održavanje. Preporuka je i provođenje edukacija u polju za vinogradare o biologiji ovog štetnika.

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Acquisition capability of the grapevine Flavescence dorée by the leafhopper vector Scaphoideus titanus Ball correlates with phytoplasma titre in the source plant

Journal of Pest Science ◽

10.1007/s10340-014-0593-3 ◽

2014 ◽

Vol 87 (4) ◽

pp. 671-679 ◽

Cited By ~ 31

Author(s):

Luciana Galetto ◽

Dimitrios Miliordos ◽

Chiara Roggia ◽

Mahnaz Rashidi ◽

Dario Sacco ◽

...

Keyword(s):

Leafhopper Vector ◽

Flavescence Dorée ◽

Scaphoideus Titanus ◽

Source Plant

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Models Applied to Grapevine Pests: A Review

Insects ◽

10.3390/insects12020169 ◽

2021 ◽

Vol 12 (2) ◽

pp. 169

Author(s):

Federico Lessio ◽

Alberto Alma

Keyword(s):

Lobesia Botrana ◽

Grape Berry ◽

Homalodisca Vitripennis ◽

Flavescence Dorée ◽

Or Groups ◽

Scaphoideus Titanus ◽

Different Types ◽

Computer Based ◽

Biological Aspects ◽

Definition Of

This paper reviews the existing predictive models concerning insects and mites harmful to grapevine. A brief conceptual description is given on the definition of a model and about different types of models: deterministic vs. stochastics, continuous vs. discrete, analytical vs. computer-based, and descriptive vs. data-driven. The main biological aspects of grapevine pests covered by different types of models are phenology, population growth and dynamics, species distribution, and invasion risk. A particular emphasis is put on forecasting epidemics of plant disease agents transmitted by insects with sucking-piercing mouthparts. The most investigated species or groups are the glassy-winged sharpshooter Homalodisca vitripennis (Germar) and other vectors of Xylella fastidiosa subsp. fastidiosa, a bacterium agent of Pierce’s disease; the European grape berry moth, Lobesia botrana (Denis and Schiffermuller); and the leafhopper Scaphoideus titanus Ball, the main vector of phytoplasmas agents of Flavescence dorée. Finally, the present and future of decision-support systems (DSS) in viticulture is discussed.

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Scaphoideus titanus Ball, 1932, e Homalodisca vitripennis (Germar, 1821) (Hemiptera, Cicadellidae): due specie di temuta introduzione in Sicilia

Biogeographia – The Journal of Integrative Biogeography ◽

10.21426/b630110581 ◽

2011 ◽

Vol 30 ◽

Author(s):

Federico Lessio ◽

Chiara Ferracini ◽

Alberto Alma

Keyword(s):

Homalodisca Vitripennis ◽

Scaphoideus Titanus

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Acquisition of Flavescence Dorée Phytoplasma by Scaphoideus titanus Ball from Different Grapevine Varieties

International Journal of Molecular Sciences ◽

10.3390/ijms17091563 ◽

2016 ◽

Vol 17 (9) ◽

pp. 1563 ◽

Cited By ~ 13

Author(s):

Luciana Galetto ◽

Dimitrios Miliordos ◽

Mattia Pegoraro ◽

Dario Sacco ◽

Flavio Veratti ◽

...

Keyword(s):

Flavescence Dorée ◽

Scaphoideus Titanus

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Reduced fitness of the leafhopper vector Scaphoideus titanus exposed to Flavescence doree phytoplasma

Entomologia Experimentalis et Applicata ◽

10.1111/j.1570-7458.2005.00240.x ◽

2005 ◽

Vol 115 (2) ◽

pp. 283-290 ◽

Cited By ~ 41

Author(s):

Alberto Bressan ◽

Vincenzo Girolami ◽

Elisabeth Boudon-Padieu

Keyword(s):

Leafhopper Vector ◽

Flavescence Dorée ◽

Scaphoideus Titanus

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First Report of Flavescence Dorée-Related Phytoplasma Affecting Grapevines in Croatia

Plant Disease ◽

10.1094/pdis-09-10-0664 ◽

2011 ◽

Vol 95 (3) ◽

pp. 353-353 ◽

Cited By ~ 7

Author(s):

M. Šeruga Musić ◽

D. Škorić ◽

I. Haluška ◽

I. Križanac ◽

J. Plavec ◽

...

Keyword(s):

Nested Pcr ◽

Reference Strain ◽

Simultaneous Detection ◽

Pinot Noir ◽

Vitis Vinifera L ◽

Rrna Gene ◽

Pcr Assay ◽

Direct Pcr ◽

Flavescence Dorée ◽

Scaphoideus Titanus

Flavescence dorée (FD) and Bois noir (BN) phytoplasmas are principal grapevine yellows (GY) agents in the wider Euro-Mediterranean Region. While BN phytoplasma belongs to the ribosomal subgroup 16SrXII-A, the FD agents belong either to the ribosomal subgroups 16SrV-C or -D. During the official GY survey in 2009, 40 symptomatic grapevines (Vitis vinifera L.) were sampled throughout grapevine-growing regions in Croatia. Typical GY symptoms of leaf yellowing or reddening were evident on white and red varieties, respectively. Leaf rolling as well as irregular lignification of the shoots and withering of clusters were also observed. Phloem tissue from cuttings and leaf veins from mature vines were sampled for total DNA extraction and amplification of phytoplasma 16S rRNA gene by using generic primers P1/P7 in a direct PCR assay followed by a nested PCR using primer pair R16F2n/R2 (2). Phytoplasma ribosomal group affiliation was determined by restriction fragment length polymorphism (RFLP) analysis of the nested PCR products with enzyme Tru1I (Fermentas, Vilnius, Lithuania). These initial findings were validated and augmented by a triplex real-time PCR assay targeting the nonribosomal map gene. This assay enables simultaneous detection of BN and FD (16SrV-C and -D) phytoplasmas in grapevine (3). Assay results revealed the majority of GY positive vines (19 of 40) contained BN phytoplasma which is widespread. For the first time in Croatia, two red variety samples, Pinot Noir and Plemenka Crvena, from the vicinity of Ozalj (Vivodina) and Zagreb (Brezje), respectively, were found to harbor FD-related phytoplasmas. Fragments amplified by P1/P7 primers from latter samples were cloned and sequenced. Sequence analyses using online interactive tool iPhyClassifier (4) revealed that the phytoplasma under study from Pinot Noir sample (GenBank Accession No. HQ712064) is a member of 16SrV-C subgroup and shares 99.87% similarity with 16S rDNA sequence of the reference strain (GenBank Accession No. AF176319). The sequence from the Plemenka Crvena sample (GenBank Accession No. HQ712065) shares 99.54% similarity with the reference strain and has the most similar virtual RFLP pattern to the one of the 16SrV-C subgroup (GenBank Accession No. AY197642). These findings are currently limited to vineyards in northwestern Croatia. Even so, the presence of FD principal cicadellid vector Scaphoideus titanus in the country and the occurrence and distribution of FD in neighboring countries (1,2) are factors indicating that the spread of FD in Croatia is highly probable. References: (1) L. Filippin et al. Plant Pathol. 58:826, 2009. (2) S. Kuzmanović et al. Vitis 47:105, 2008. (3) C. Pelletier et al. Vitis 48:87, 2009. (4) Y. Zhao et al. Int. J. Syst. Evol. Microbiol. 59:2582, 2009.

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Silencing of ATP Synthase β Impairs Egg Development in the Leafhopper Scaphoideus titanus, Vector of the Phytoplasma Associated with Grapevine Flavescence Dorée

International Journal of Molecular Sciences ◽

10.3390/ijms23020765 ◽

2022 ◽

Vol 23 (2) ◽

pp. 765

Author(s):

Matteo Ripamonti ◽

Luca Cerone ◽

Simona Abbà ◽

Marika Rossi ◽

Sara Ottati ◽

...

Keyword(s):

Atp Synthase ◽

Cathepsin L ◽

Field Application ◽

Female Sterility ◽

Model Species ◽

Egg Development ◽

Flavescence Dorée ◽

Vector Population ◽

Regulatory Constraints ◽

Scaphoideus Titanus

Scaphoideus titanus (Hemiptera: Cicadellidae) is the natural vector of Flavescence dorée phytoplasma, a quarantine pest of grapevine with severe impact on European viticulture. RNA interference (RNAi) machinery components are present in S. titanus transcriptome and injection of ATP synthase β dsRNAs into adults caused gene silencing, starting three days post injection (dpi) up to 20 dpi, leading to decrease cognate protein. Silencing of this gene in the closely related leafhopper Euscelidiusvariegatus previously showed female sterility and lack of mature eggs in ovaries. Here, alteration of developing egg morphology in S. titanus ovaries as well as overexpression of hexamerin transcript (amino acid storage protein) and cathepsin L protein (lysosome proteinase) were observed in dsATP-injected females. To evaluate RNAi-specificity, E.variegatus was used as dsRNA-receiving model-species. Different doses of two sets of dsRNA-constructs targeting distinct portions of ATP synthase β gene of both species induced silencing, lack of egg development, and female sterility in E. variegatus, indicating that off-target effects must be evaluated case by case. The effectiveness of RNAi in S. titanus provides a powerful tool for functional genomics of this non-model species and paves the way toward RNAi-based strategies to limit vector population, despite several technical and regulatory constraints that still need to be overcome to allow open field application.

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Leafhoppers and cixiids in phytoplasma-infected carrot fields: Species composition and potential phytoplasma vectors

Pesticidi i fitomedicina ◽

10.2298/pif1004311d ◽

2010 ◽

Vol 25 (4) ◽

pp. 311-318 ◽

Cited By ~ 7

Author(s):

Tanja Drobnjakovic ◽

Pantelija Peric ◽

Dejan Marcic ◽

Luca Picciau ◽

Alberto Alma ◽

...

Keyword(s):

Control Strategies ◽

Species Level ◽

Effective Control ◽

Correct Identification ◽

Vegetable Crops ◽

Vegetative Period ◽

Aster Yellows ◽

Scaphoideus Titanus ◽

The Family ◽

Pcr Rflp

The first molecular analysis of samples collected in southern Backa (Serbia) confirmed the presence of aster yellows (16SrI) and stolbur phytoplasmas (16SrXII) in insects belonging to the family Cicadellidae, as well as in carrot plants where the insects were collected. A correct identification of the phytoplasmas and their vectors is essential to arrange effective control strategies to prevent diseases associated with phytoplasmas from spreading to carrots and other vegetable crops. In order to enhance knowledge about insect vectors of aster yellows and stolbur phytoplasmas in Serbia, Cicadellidae and Cixiidae (Homoptera Auchenorrhyncha), the most common vectors of these phytoplasmas, were monitored in southern Backa during 2008. Adults leaf- and planthoppers were collected and identified at species level using standard entomological methods, and tested for phytoplasma presence by means of PCR/RFLP. A total of 13 insect species of Cicadellidae were identified, as follows: a) three species of the subfamily Agallinae: Anaceratagallia ribauti (Ossiannilsson), Anaceratagallia venosa (Fourcroy), and Anaceratagallia laevis (Ribaut); b) seven species of the subfamily Deltocephalinae: Psammotettix confinis (Dahlbom), Psammotettix striatus (Linnaues) Psammottettix alienus (Dahlbom), Macrosteles sexnotatus (Fall?n), Ophiola decumana (Kontkanen), Errastunus ocellaris Fall?n, and Scaphoideus titanus Ball; c) three species of the subfamily Typhlocibinae: Eupteryx atropunctata (Goeze), Eupteryx mellissae Curtis, Zyginidia pullula (Boheman). Female specimens of the genus Euscelis (Deltocephalinae) were also collected, as well as one species of Reptalus quinquecostatus (Dufour) of the family Cixiidae. Stolbur phytoplasmas were detected in A. laevis, A. ribauti, A. venosa, P. striatus, P. confinis and P. alienus. The species: A. laevis, O. decumana, and P. confinis were AY-infected (subgroup 16SrI-A), while subgroup 16SrI-C was found only in one specimen of P. confinis. Since some aster yellows- and stolbur-infected species of the genera Psammotettix and Anaceratagallia (especially P. confinis and A. laevis) were regularly and commonly found in the infected carrot fields during the whole vegetative period, they could play a significant role in transmitting and spreading these pathogens in natural environment.

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