Optimization Studies on Extracellular Lipase Production from Penicillium citrinum.

2020 ◽  
Vol 12 (03) ◽  
Keyword(s):  
Lipase Production ◽  
Penicillium Citrinum ◽  
Extracellular Lipase

scholarly journals Production, Purification and Characterization of Extracellular Lipase from a Mutated Strain of Penicillium Citrinum KU613360

2021 ◽  
pp. 111-120
Author(s):  
Lakkakula Bhagya Lakshmi ◽  
M Raghu Ram
Keyword(s):  
Wild Strain ◽  
Strain Improvement ◽  
Lipase Production ◽  
Industrial Applications ◽  
Penicillium Citrinum ◽  
Extracellular Lipase ◽  
Purification And Characterization ◽  
Sds Page ◽  
Guntur District

In the present study lipase production, purification and characterization were carried out with a novel fungal strain of Penicillium citrinum KU613360 isolated from vegetable oil contaminated soil samples collected from oil mills located in and around Guntur District, Andhra Pradesh, India. The strain improvement was carried out by subjecting the strain to both UV and Ethidium Bromide treatments. The wild strain of P. citrinum KU613360 showed maximum lipase activity of 1.053±0.32IUmL-1 on optimized medium and while the mutated strain treated with combination of UV (300 sec) and Et Br (200 µgcm3), recorded the enzyme activity of 4.260±0.011IUmL-1, using the optimised medium at 6.5 pH and 40°C temperature. Thus, a 404% enhancement in the activity was achieved by using induced mutation on wild strain of P. citrinum KU613360. The molecular weight of the purified lipase from the mutated strain was found to be 35 kDa, when analysed on SDS PAGE. From our results it was concluded that the mutated strain has considerable capability and potentiality to be used in various industrial applications.

Repression ofPseudomonas fluorescensextracellular lipase secretion by arginine

1990 ◽  
Vol 57 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Leonides Fernandez ◽  
Carmen San Jose ◽  
Robin C. McKellar
Keyword(s):  
Amino Acids ◽  
Methyl Ester ◽  
Glutamic Acid ◽  
Pseudomonas Fluorescens ◽  
Lipase Production ◽  
Extracellular Lipase ◽  
Metabolic Intermediate ◽  
Proteinase Production

SummaryThe effect of arginine on the production of extracellular lipase byPseudomonas fluorescens32A was studied. Arginine repressed lipase production when N was supplied partially or totally as arginine. Proteinase production was unaffected under the same conditions. Arginine did not repress lipase secretion when cells were pregrown in an arginine-containing medium; however, repression was found with uninduced cells. Several arginine-analogues were tested for the ability to repress lipase secretion and. of these, L-homoarginine and L-canavanine were the most effective. D-Arginine, L-arginine methyl ester, and L-ornithine did not cause repression. With the exception of glutamic acid and methionine, those amino acids that supplied N but not C for growth were found to repress lipase secretion. It is suggested that the accumulation of metabolic intermediate(s) may cause repression of lipase production.

Extracellular Lipase Production by Fungi from Sunflower Seed

Mycologia ◽  
1987 ◽  
Vol 79 (2) ◽  
pp. 265 ◽  
Author(s):  
R. G. Roberts ◽  
W. H. Morrison ◽  
J. A. Robertson ◽  
R. T. Hanlin
Keyword(s):  
Sunflower Seed ◽  
Lipase Production ◽  
Extracellular Lipase

scholarly journals Selection and optimization of extracellular lipase production using agro-industrial waste

2014 ◽  
Vol 13 (4) ◽  
pp. 566-573 ◽  
Author(s):  
Rafaella Mello Bueno Pabline ◽  
Ferreira de Oliveira Tatianne ◽  
Caliari, Gabriel Luis Castiglioni Mrcio ◽  
Soares Soares Jnior Manoel
Keyword(s):  
Industrial Waste ◽  
Lipase Production ◽  
Extracellular Lipase

The lipase activity of Talaromyces emersonii

1978 ◽  
Vol 56 (16) ◽  
pp. 1840-1843 ◽  
Author(s):  
B. A. Oso
Keyword(s):  
Liquid Medium ◽  
Yeast Extract ◽  
Lipase Activity ◽  
Natural Habitat ◽  
Carbon Sources ◽  
Lipase Production ◽  
Extracellular Lipase ◽  
Talaromyces Emersonii ◽  
Stationary Liquid ◽  
Presence And Absence

Studies were carried out on the ability of Talaromyces emersonii Stolk to produce extracellular lipase in stationary liquid medium under various conditions. The best temperatures for lipase synthesis and activity were 40–45 °C, and at all the temperatures (37–55 °C) tested for lipase production, maximum enzyme was produced 8 days after incubation. Lipase synthesis was induced when various carbohydrates were used as carbon sources both in the presence and absence of yeast extract. The significance of lipase production in relation to the natural habitat is discussed.

scholarly journals Soybean Fraction Increasing the Extracellular Lipase Production bySaccharomycopsis iipolytica(Candida paralipolytica)

1978 ◽  
Vol 42 (10) ◽  
pp. 1937-1978
Author(s):  
Yasuhide Ota ◽  
Yuki Morimoto ◽  
Tsutomu Sugiura ◽  
Yasuji Minoda
Keyword(s):  
Lipase Production ◽  
Extracellular Lipase
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