The lipase activity of Talaromyces emersonii

1978 ◽  
Vol 56 (16) ◽  
pp. 1840-1843 ◽  
Author(s):  
B. A. Oso
Keyword(s):  
Liquid Medium ◽  
Yeast Extract ◽  
Lipase Activity ◽  
Natural Habitat ◽  
Carbon Sources ◽  
Lipase Production ◽  
Extracellular Lipase ◽  
Talaromyces Emersonii ◽  
Stationary Liquid ◽  
Presence And Absence

Studies were carried out on the ability of Talaromyces emersonii Stolk to produce extracellular lipase in stationary liquid medium under various conditions. The best temperatures for lipase synthesis and activity were 40–45 °C, and at all the temperatures (37–55 °C) tested for lipase production, maximum enzyme was produced 8 days after incubation. Lipase synthesis was induced when various carbohydrates were used as carbon sources both in the presence and absence of yeast extract. The significance of lipase production in relation to the natural habitat is discussed.

Effects of carbon sources on extracellular lipase production and lip A transcription in Acinetobacter calcoaceticus

2000 ◽  
Vol 24 (1) ◽  
pp. 25-30 ◽  
Author(s):  
G F Mahler ◽  
R G Kok ◽  
A Cordenons ◽  
K J Hellingwerf ◽  
B C Nudel
Keyword(s):  
Carbon Sources ◽  
Acinetobacter Calcoaceticus ◽  
Lipase Production ◽  
Extracellular Lipase

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by Aspergillus niger

2016 ◽  
Vol 60 ◽  
pp. 18-29 ◽  
Author(s):  
Ahmed I. El-Batal ◽  
Ayman A. Farrag ◽  
Mohamed A. Elsayed ◽  
Ahmed M. El-Khawaga
Keyword(s):  
Aspergillus Niger ◽  
Incubation Temperature ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Lipase Production ◽  
Extracellular Lipase ◽  
Chemical Parameters ◽  
Physical And Chemical Parameters ◽  
Physical And Chemical

Abstract- The present investigation was carried out to evaluate the effect of different growth conditions on lipase production byAspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimization of physical and chemical parameters was done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 – 9), incubation temperature (20 – 30 °C), incubation time (8 – 80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity byAspergillusnigerwas 200 rpm.

scholarly journals Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

2018 ◽  
Vol 5 (3) ◽  
pp. 135-141 ◽  
Author(s):  
. Shreya ◽  
Arun Kumar Sharma ◽  
Vinay Sharma ◽  
Jyoti Saxena
Keyword(s):  
Lipase Activity ◽  
Specific Activity ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Potassium Nitrate ◽  
Lipase Production ◽  
Culture Conditions ◽  
Mustard Oil ◽  
Malt Extract ◽  
Inorganic Nitrogen Source

The present study aimed to optimize culture conditions for optimal growth and production of extracellular lipase. Lipolytic fungal strain named as S3St2 previously isolated from a petrol pump soil sample of Newai Town was used for optimization study. Among the tested carbohydrate carbon sources, polysaccharide-starch exhibited maximum lipase production (21.25±0.70 IU/ml/min) with highest specific activity (1.47±0.06 U/mg). Lipase activity and specific activity were higher with mustard oil 1 % (v/v) among all lipidic carbon sources. Among inorganic nitrogen source, potassium nitrate was found best inducer of lipase activity, malt extract supported the fungus growth (dry weight of cell pellets was 0.467 g) and exhibited maximum lipase activity among all organic nitrogen sources. Lipase activity was optimum at pH 8.0, indicates alkalophillic nature of production media supports the growth of fungus. Higher lipase activity (27.92±0.87 IU/ml/min) was detected at 28ºC. The incubation time of 5 days was found optimum for maximum lipase production (31.51±0.21 IU/ml/min).

Extracellular lipase production by a sapwood-staining fungus, Ophiostoma piceae

1995 ◽  
Vol 11 (6) ◽  
pp. 638-642 ◽  
Author(s):  
Y. Gao ◽  
C. Breuil
Keyword(s):  
Lipase Production ◽  
Extracellular Lipase ◽  
Ophiostoma Piceae

scholarly journals Factorial Design to Optimize Biosurfactant Production byYarrowia lipolytica

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Gizele Cardoso Fontes ◽  
Priscilla Filomena Fonseca Amaral ◽  
Marcio Nele ◽  
Maria Alice Zarur Coelho
Keyword(s):  
Surface Tension ◽  
Factorial Design ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Biosurfactant Production ◽  
Standard Process ◽  
Emulsification Index ◽  
Full Factorial

In order to improve biosurfactant production byYarrowia lipolyticaIMUFRJ 50682, a factorial design was carried out. A24full factorial design was used to investigate the effects of nitrogen sources (urea, ammonium sulfate, yeast extract, and peptone) on maximum variation of surface tension (ΔST) and emulsification index (EI). The best results (67.7% of EI and 20.9 mNm−1ofΔST) were obtained in a medium composed of 10 g 1−1of ammonium sulfate and 0.5 g 1−1of yeast extract. Then, the effects of carbon sources (glycerol, hexadecane, olive oil, and glucose) were evaluated. The most favorable medium for biosurfactant production was composed of both glucose (4% w/v) and glycerol (2% w/v), which provided an EI of 81.3% and aΔST of 19.5 mN m−1. The experimental design optimization enhancedΔEI by 110.7% andΔST by 108.1% in relation to the standard process.

scholarly journals Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: the process comparison analysis by statistical approach

2013 ◽  
Vol 67 (4) ◽  
pp. 677-685 ◽  
Author(s):  
Sonja Jakovetic ◽  
Zorica Knezevic-Jugovic ◽  
Sanja Grbavcic ◽  
Dejan Bezbradica ◽  
Natasa Avramovic ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lipase Activity ◽  
Sunflower Oil ◽  
Hydrolytic Enzymes ◽  
Tween 80 ◽  
Lipase Production ◽  
Fermentation Time ◽  
Process Comparison ◽  
Central Composite Experimental Design ◽  
Oil Concentration

Pseudomonas aeruginosa was repeatedly reported as powerful producer of rhamnolipid biosurfactants as well as producer of hydrolytic enzymes. In this study effects of four fermentation factors were evaluated using response surface methodology and experiments were performed in accordance with a four-factor and five-level central composite experimental design. Investigated factors were: fermentation temperature, time of fermentation, concentration of sunflower oil and concentration of Tween? 80. The most important finding was that regression coefficients of the highest values were those that describe interactions between factors and that they differ for lipase and rhamnolipid production, which were both investigated in this study. Production of both metabolites was optimized and response equations were obtained, making it possible to predict rhamnolipid concentration or lipase activity from known values of the four factors. The highest achieved rhamnolipid concentration and lipase activity were 138 mg dm-3 (sunflower oil concentration 0.8 %, Tween? 80 concentration 0.05 %, temperature 30?C, and fermentation time 72 h) and 11111 IU dm-3(sunflower concentration of 0.4 %, Tween? 80 concentration of 0.05 %, temperature of 30?C, and fermentation time of 120 h), respectively.

scholarly journals Optimization of Lipase Production in Solid-State Fermentation by Rhizopus Arrhizus in Nutrient Medium Containing Agroindustrial Wastes

2018 ◽  
Vol 12 (1) ◽  
pp. 189-203 ◽  
Author(s):  
Georgi Dobrev ◽  
Hristina Strinska ◽  
Anelia Hambarliiska ◽  
Boriana Zhekova ◽  
Valentina Dobreva
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Lipase Activity ◽  
Nutrient Medium ◽  
Solid Medium ◽  
Low Cost ◽  
Lipase Production ◽  
Rhizopus Arrhizus ◽  
Agroindustrial Wastes

Background: Rhizopus arrhizus is a potential microorganism for lipase production. Solid-state fermentation is used for microbial biosynthesis of enzymes, due to advantages, such as high productivity, utilization of abundant and low-cost raw materials, and production of enzymes with different catalytic properties. Objective: The objective of the research is optimization of the conditions for lipase production in solid-state fermentation by Rhizopus arrhizus in a nutrient medium, containing agroindustrial wastes. Method: Biosynthesis of lipase in solid-state fermentation by Rhizopus arrhizus was investigated. The effect of different solid substrates, additional carbon and nitrogen source, particles size and moisture content of the medium on enzyme production was studied. Response surface methodology was applied for determination of the optimal values of moisture content and tryptone concentration. A procedure for efficient lipase extraction from the fermented solids was developed. Results: Highest lipase activity was achieved when wheat bran was used as a solid substrate. The addition of 1% (w/w) glucose and 5% (w/w) tryptone to the solid medium significantly increased lipase activity. The structure of the solid medium including particles size and moisture content significantly influenced lipase production. A mathematical model for the effect of moisture content and tryptone concentration on lipase activity was developed. Highest enzyme activity was achieved at 66% moisture and 5% (w/w) tryptone. The addition of the non-ionic surfactant Disponyl NP 3070 in the eluent for enzyme extraction from the fermented solids increased lipase activity about three folds. Conclusion: After optimization of the solid-state fermentation the achieved 1021.80 U/g lipase activity from Rhizopus arrhizus was higher and comparable with the activity of lipases, produced by other fungal strains. The optimization of the conditions and the use of low cost components in solid-state fermentation makes the process economicaly effective for production of lipase from the investigated strain Rhizopus arrhizus.

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