scholarly journals SPORULATION OF SPOROSARCENA PASTEURII (BACILLUS PASTEURII) ON DIFFERENT NUTRIENT MEDIUM IN LABORATORY CONDITIONS

2020 ◽  
Vol 7 (13) ◽  
Keyword(s):  
Nutrient Medium ◽  
Bacillus Pasteurii ◽  
Laboratory Conditions

scholarly journals An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria

2021 ◽  
Vol 37 ◽  
pp. 00063
Author(s):  
Vladimir Ermakov ◽  
Nikolay Titov
Keyword(s):  
Hydrogen Sulfide ◽  
Nutrient Medium ◽  
Culture Media ◽  
Laboratory Conditions ◽  
Environmental Objects ◽  
Single Section ◽  
Single Compartment ◽  
Petri Dishes ◽  
Ferment Lactose ◽  
Medium Formulation

In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carried out according to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The environment can also be used for conducting sanitary and microbiological studies of environmental objects. The environment can be used to perform the ONPG test. In the course of preparing the modified Drigalski agar for operation in laboratory conditions, we recommend using the medium according to one of the options: when one of the carbohydrates is added, the medium is poured into single-section reusable or disposable petri dishes; when two carbohydrates are added, the medium is poured into two-section reusable or disposable petri dishes; when a complex of carbohydrates lactose + mannitol and glucose + sucrose is added, the medium is poured into two-section reusable or disposable petri dishes; when using all four drives separately, the medium is dispensed into single-compartment reusable or disposable petri dishes. We recommend preparing modified Drigalski agar at a plant for the production of ready-made culture media with filling the medium in two-section petri dishes (complex of carbohydrates lactose + mannitol in one section, glucose + sucrose in another section), or with filling the medium in four-section petri dishes (with each carbohydrate in separate section).

In vitro scaling of Tagetes patula L. root culture

2020 ◽  
Vol 19 (1) ◽  
pp. 255-259
Author(s):  
L. P. Khlebova ◽  
E. S. Brovko
Keyword(s):  
Growth Rate ◽  
Hairy Roots ◽  
Growth Pattern ◽  
Nutrient Medium ◽  
Root Culture ◽  
Growth Cycle ◽  
Tagetes Patula ◽  
Laboratory Conditions

The features of the in vitro accumulation of biomass of Tagetes patula L. root culture in the process ofscaling under laboratory conditions in different volumes of the nutrient medium and culture vessels were studied. It wasrevealed that the growth pattern of the hairy roots line and its morphology depend on the volume of the incubation vessel.The pattern of increasing the growth rate and lengthening of the growth cycle of the culture in larger flasks (up to 500 ml)was established.

Observations on the pupal and adult stages of Cuterebra emasculator Fitch (Diptera: Cuterebridae)

1972 ◽  
Vol 50 (11) ◽  
pp. 1367-1372 ◽  
Author(s):  
Gordon F. Bennett
Keyword(s):  
Nutrient Medium ◽  
Field Conditions ◽  
Laboratory Conditions ◽  
Complete Metamorphosis

Metamorphosis of Cuterebra emasculator was enhanced by subjecting puparia to temperatures of 43 ± 3 °F for 120 days. Under laboratory conditions, complete metamorphosis required 219 ± 5 days; under field conditions, required 330(303–340) days. Larvae and puparia producing male flies were 20–30% lighter in weight than those producing females. Male flies lived for 6–7 days in the laboratory and up to 22 (mean of 14) days if injected with a nutrient medium. Females lived for 7–8 days in the laboratory and about 14 days in the field; fully formed eggs were present in the ovarioles on the sixth day after emergence from the puparium in the laboratory. Attempts to induce mating and oviposition in both the laboratory and the field were unsuccessful. Female flies did not appear to be attracted by the presence of the host or host excretory/fecal products.

scholarly journals Differentiation of valuable for breeding sunflower lines by resistance to Phoma rot

2021 ◽  
Vol 937 (3) ◽  
pp. 032014
Author(s):  
S L Saukova ◽  
T S Antonova ◽  
N M Araslanova ◽  
M V Iwebor ◽  
E N Ryzhenko
Keyword(s):  
Root System ◽  
Nutrient Medium ◽  
Point Estimation ◽  
Spore Suspension ◽  
Artificial Inoculation ◽  
Leaf Petiole ◽  
Sunflower Plant ◽  
Suspension Concentration ◽  
Laboratory Conditions ◽  
Fungus Spore

Abstract The purpose of the research is differentiation of valuable for breeding sunflower lines by resistance to Phoma rot. The promising constant sunflower lines were estimated by their resistance to Phoma rot using methods of artificial inoculation in the greenhouse and laboratory conditions. In greenhouse conditions, there were used two methods of artificial inoculation with a fungus Plenodomus lindquistii in the bottom of leaf petiole of a sunflower plant at a stage of the first true leaf pair. Application of the pieces of agar nutrient medium with mycelium, pycnidia and picnospores (unbroken) allowed to identify resistant and mid-resistant sunflower lines (VK 900, L 86 and L 107, L 132). At inoculation with 0.2 ml of a fungus spore suspension (concentration of 1 × 106 spores/ml), five susceptible and one very susceptible sunflower lines (L 2385, L 103, L 136, L 131, L 128, and L 82) were determined. In laboratory conditions, the quick test for estimation of susceptibility to Phoma rot (dose of infection – 12 g) allowed to reveal more than 50 % of plants with healthy root system and two constant sunflower lines L 116 and L 136 with the affection degree of one point. Estimation of susceptibility to Phoma rot infection of the different organs (stem and root system) at artificial inoculation showed a possibility to differentiate sunflower lines by resistance of roots and stems and to select the most promising for the further work in breeding.

Human drug taking under controlled laboratory conditions.

1988 ◽  
Author(s):  
Marian W. Fischman ◽  
Richard W. Foltin ◽  
Joseph V. Brady
Keyword(s):  
Laboratory Conditions

Multiple recycling of paperboard: Paperboard characteristics and maximum number of recycling cycles— Part I: Multiple recycling of corrugated base paper

TAPPI Journal ◽  
2019 ◽  
Vol 18 (11) ◽  
pp. 631-638
Author(s):  
FREDERIC KREPLIN ◽  
HANS-JOACHIM PUTZ ◽  
SAMUEL SCHABEL
Keyword(s):  
Compression Test ◽  
Production Capacity ◽  
Strength Properties ◽  
Paper Properties ◽  
Corrugated Board ◽  
Laboratory Conditions ◽  
Base Paper ◽  
Study Results ◽  
Short Span ◽  
Multiple Recycling

Paper for recycling is an important fiber source for the production of corrugated base paper. The change in production capacity toward more and more packaging papers affects the composition of paper for recycling and influences the paper quality. This research project investigated the influence of the multiple recycling of five different corrugated base papers (kraftliner, neutral sulfite semichemical [NSSC] fluting, corrugating medium, testliner 2, and testliner 3) on suspension and strength properties under laboratory conditions. The corrugated board base papers were repulped in a low consistency pulper and processed into Rapid-Köthen laboratory sheets. The sheets were then recycled up to 15 times in the same process. In each cycle, the suspension and the paper properties were recorded. In particular, the focus was on corrugated board-specific parameters, such as short-span compression test, ring crush test, corrugating medium test, and burst. The study results indicate how multiple recycling under laboratory conditions affects fiber and paper properties.

Mechanoactivation Of Rice Husk Ash In Laboratory Conditions

2019 ◽  
pp. 20-26
Keyword(s):  
Mechanical Activation ◽  
Rice Husk ◽  
Amorphous Silica ◽  
Silicon Oxide ◽  
Rice Husk Ash ◽  
Pozzolanic Activity ◽  
Maximum Solubility ◽  
Laboratory Conditions ◽  
Before And After ◽  
Mineral Additive

In many rice producing countries of the world, including in Vietnam, various research aimed at using rice husk ash (RHA) as a finely dispersed active mineral additive in cements, concrete and mortars are being conducted. The effect of the duration of the mechanoactivation of the RHA, produced under laboratory conditions in Vietnam, on its pozzolanic activity were investigated in this study. The composition of ash was investigated by laser granulometry and the values of indicators characterizing the dispersion of its particles before and after mechanical activation were established. The content of soluble amorphous silicon oxide in rice husk ash samples was determined by photocolorimetric analysis. The pizzolanic activity of the RHA, fly ash and the silica fume was also compared according to the method of absorption of the solution of the active mineral additive. It is established that the duration of the mechanical activation of rice husk ash by grinding in a vibratory mill is optimal for increasing its pozzolanic activity, since it simultaneously results in the production of the most dispersed ash particles with the highest specific surface area and maximum solubility of the amorphous silica contained in it. Longer grinding does not lead to further reduction in the size of ash particles, which can be explained by their aggregation, and also reduces the solubility of amorphous silica in an aqueous alkaline medium.

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