Observations on the pupal and adult stages of Cuterebra emasculator Fitch (Diptera: Cuterebridae)

1972 ◽  
Vol 50 (11) ◽  
pp. 1367-1372 ◽  
Author(s):  
Gordon F. Bennett

Metamorphosis of Cuterebra emasculator was enhanced by subjecting puparia to temperatures of 43 ± 3 °F for 120 days. Under laboratory conditions, complete metamorphosis required 219 ± 5 days; under field conditions, required 330(303–340) days. Larvae and puparia producing male flies were 20–30% lighter in weight than those producing females. Male flies lived for 6–7 days in the laboratory and up to 22 (mean of 14) days if injected with a nutrient medium. Females lived for 7–8 days in the laboratory and about 14 days in the field; fully formed eggs were present in the ovarioles on the sixth day after emergence from the puparium in the laboratory. Attempts to induce mating and oviposition in both the laboratory and the field were unsuccessful. Female flies did not appear to be attracted by the presence of the host or host excretory/fecal products.

Author(s):  
Ovidiu RANTA ◽  
Ioan DROCAS ◽  
Sorin STANILA ◽  
Adrian MOLNAR ◽  
Mircea Valentin MUNTEAN ◽  
...  

Autors was designed a system to modify the SPC romanian seeding machine for in order that it can be used for no-till technology. This machine was manufactured with the help of S.C. MECANICA M.A.R.I..U.S. S.A. in Cluj- Napoca and it was used in laboratory conditions in a state of the art soil bin of Hohenheim University, Stuttgart and in laboratory-field conditions. The field experiments were located on a plot of Experimental Teaching Facility of USAMV Cluj-Napoca, on aluviosol molic soil after SRTS – 200, in location Lunca Someşului Mic (Podişul Someşan) .


2007 ◽  
Vol 37 (6) ◽  
pp. 1524-1528 ◽  
Author(s):  
Charles Ira Abramson ◽  
Paulo Alves Wanderley ◽  
Alexandre José Soares Miná ◽  
Maria José Araújo Wanderley

This research was aimed at obtaining data about earwig behavior (Marava arachidis Y.) on fennel agro-ecosystems and evaluating its potential capacity to access plants in the absence of aphids under laboratory and field conditions. This study establishes a baseline to evaluate earwigs as biological controls to combat aphids that attack fennel plants. Two experiments were developed. In experiment 1, earwigs were studied under laboratory conditions, in experiment 2 under field conditions. Independent variables were sex, exposure to the essential oil of fennel for 24 or 48 hours, and whether the fennel plant was vegetative or flowering. The results indicated that earwigs will climb a fennel plant in the absence of aphids and that few statistical significant results were obtained among the independent variables examined. A difference between male and females was noted in the field experiment in animals receiving 48 h of exposure to the essential oil of fennel. The terminal height reached by males and females in the 48 h vegetative and flower condition also differed.


Information ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 95 ◽  
Author(s):  
Kaizhou Li ◽  
Jianhui Lin ◽  
Jinrong Liu ◽  
Yandong Zhao

Diseases from Ginkgo biloba have brought great losses to medicine and the economy. Therefore, if the degree of disease can be automatically identified in Ginkgo biloba leaves, people will take appropriate measures to avoid losses in advance. Deep learning has made great achievements in plant disease identification and classification. For this paper, the convolution neural network model was used to classify the different degrees of ginkgo leaf disease. This study used the VGGNet-16 and Inception V3 models. After preprocessing and training 1322 original images under laboratory conditions and 2408 original images under field conditions, 98.44% accuracy was achieved under laboratory conditions and 92.19% under field conditions with the VGG model. The Inception V3 model achieved 92.3% accuracy under laboratory conditions and 93.2% under field conditions. Thus, the Inception V3 model structure was more suitable for field conditions. To our knowledge, there is very little research on the classification of different degrees of the same plant disease. The success of this study will have a significant impact on the prediction and early prevention of ginkgo leaf blight.


2014 ◽  
Vol 4 (6) ◽  
pp. 492-497 ◽  
Author(s):  
Nazaire Aïzoun ◽  
Roseric Azondekon ◽  
Rock Aïkpon ◽  
Virgile Gnanguenon ◽  
Razaki Osse ◽  
...  

2021 ◽  
Vol 37 ◽  
pp. 00063
Author(s):  
Vladimir Ermakov ◽  
Nikolay Titov

In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carried out according to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The environment can also be used for conducting sanitary and microbiological studies of environmental objects. The environment can be used to perform the ONPG test. In the course of preparing the modified Drigalski agar for operation in laboratory conditions, we recommend using the medium according to one of the options: when one of the carbohydrates is added, the medium is poured into single-section reusable or disposable petri dishes; when two carbohydrates are added, the medium is poured into two-section reusable or disposable petri dishes; when a complex of carbohydrates lactose + mannitol and glucose + sucrose is added, the medium is poured into two-section reusable or disposable petri dishes; when using all four drives separately, the medium is dispensed into single-compartment reusable or disposable petri dishes. We recommend preparing modified Drigalski agar at a plant for the production of ready-made culture media with filling the medium in two-section petri dishes (complex of carbohydrates lactose + mannitol in one section, glucose + sucrose in another section), or with filling the medium in four-section petri dishes (with each carbohydrate in separate section).


2013 ◽  
Vol 14 (4) ◽  
pp. 549
Author(s):  
Petar Chavdarov ◽  
Liliya Krasteva ◽  
Nikolaya Velcheva ◽  
Stefan Neykov

In 2012 the evaluation on the development and spread of phytopathogens, causing wilt in pepper was conducted. The observations were carried out under field conditions and natural infectious background in the Plovdiv region. In laboratory conditions were isolated and identified four phytopathogenic fungi of the genus Rhizoctonia, Fusarium, Verticillium and Phytophthora. The results of the analysis showed that the highest percentage of pepper wilt was caused by the fungus - Rhizoctonia solani.


Weed Science ◽  
1997 ◽  
Vol 45 (1) ◽  
pp. 183-189 ◽  
Author(s):  
William T. Willian ◽  
Thomas C. Mueller ◽  
Robert M. Hayes ◽  
Charles E. Snipes ◽  
David C. Bridges

Fluometuron adsorption and dissipation under field and laboratory conditions, and distribution within the soil profile was determined in 3 soils from Tennessee, Mississippi, and Georgia that are representative of the cotton-growing regions of the southeastern United States. Fluometuron adsorption was correlated with organic matter, but not with clay content or soil pH. First-order kinetics explained fluometuron dissipation under field and controlled conditions (r2≥ 0.82). Field dissipation of fluometuron was slower under dry conditions. Fluometuron was not detected below 15 cm in the soil profile in any soil, and concentrations in the 8- to 15-cm soil zone were < 15 ppbw 112 d after treatment. Fluometuron dissipation was more rapid in soil from the 0- to 8-cm depth in Tennessee soil than in Mississippi soil under controlled conditions. Dissipation was more rapid under field conditions than under laboratory conditions at 2 of 3 locations. Fluometuron half-lives in soils from the 0- to 8-cm depth ranged from 9 to 28 d under field conditions and from 11 to 43 d in the laboratory. Fluometuron dissipation in soils from 30- to 45- and 60- to 90-cm depths was not different among soils, with half-lives ranging from 58 to 99 d under laboratory conditions. Fluometuron half-life was positively correlated with soil depth and inversely correlated with organic matter. These data indicate that organic matter, soil depth, and environmental conditions affect fluometuron dissipation.


Author(s):  
L. P. Khlebova ◽  
E. S. Brovko

The features of the in vitro accumulation of biomass of Tagetes patula L. root culture in the process ofscaling under laboratory conditions in different volumes of the nutrient medium and culture vessels were studied. It wasrevealed that the growth pattern of the hairy roots line and its morphology depend on the volume of the incubation vessel.The pattern of increasing the growth rate and lengthening of the growth cycle of the culture in larger flasks (up to 500 ml)was established.


1983 ◽  
Vol 61 (4) ◽  
pp. 915-921 ◽  
Author(s):  
L. C. Lewis ◽  
J. E. Cossentine ◽  
R. D. Gunnarson

Larvae of the European corn borer, Ostrinia nubilalis (Hübner), infected with the microsporidium Nosema pyrausta (Paillot) or free of N. pyrausta infection, were fed either additional N. pyrausta, or Vairimorpha necatrix (Kramer), or a combination of N. pyrausta plus V. necatrix spores. Nosema pyrausta and (or) V. necatrix spores increased the number of tissues infected and the intensity of infection. In laboratory studies, V. necatrix, alone or in combination with N. pyrausta, killed more larvae than did N. pyrausta alone. Significantly fewer insects pupated and emerged in the V. necatrix plus N. pyrausta treatment than in the V. necatrix or N. pyrausta treatments alone. There was a correspondingly greater percentage of dead pupae in the V. necatrix plus N. pyrausta treatment. Nosema pyrausta was more detrimental under field conditions than under laboratory conditions. Of larvae surviving the winter, those that had been fed V. necatrix had significantly fewer spores per milligram of infected tissue than did larvae that had been fed N. pyrausta. These data suggest that the larvae with a higher intensity of V. necatrix spores did not survive the winter.


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