A Highly Selective Fluorescent Probe for Zn2+ Based on a Rhodamine-6G dye Derivative Modified by a Furan Unit

A fluorescent probe for Zn2+ based on rhodamine-6G has been synthesised. It showed excellent fluorescent specific selectivity and high sensitivity for Zn2+ compared with other metal ions, such as Fe3+, Cr3+, Hg2+, Ag+, Ca2+, Cu2+, Pb2+, Cd2+, Ni2+, Co2+ and Mg2+, in CH3CN–phosphate buffer saline solution. The distinct colour change and the rapid emergence of fluorescence emission provided ‘naked-eye’ detection of Zn2+. The detection limit of the fluorescent probe towards Zn2+ was 1.78 μM. In addition, the fluorescence lifetime and fluorescence quantum yield were measured.

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Using Heterocycle to Improve the Selectivity of Rhodamine-6G Dye: Synthesis of Pyrrole-Modified Rhodamine-6G and its Recognition to Zn2+

Journal of the chemical society of pakistan ◽

10.52568/000732/jcsp/41.02.2019 ◽

2019 ◽

Vol 41 (2) ◽

pp. 300-300

Author(s):

Rui Qiao Rui Qiao ◽

Hai Yun Fan Hai Yun Fan ◽

Cui Bing Bai Cui Bing Bai ◽

Ling Dai Ling Dai ◽

Lin Zhang Lin Zhang ◽

...

Keyword(s):

Rhodamine 6G ◽

Color Change ◽

Fluorescent Sensor ◽

Fluorescence Emission ◽

High Sensitivity ◽

Test Strip ◽

Recognition Mechanism ◽

Test Kit ◽

Specific Selectivity ◽

Distinct Color

The fluorescent sensor XQN for Zn2+ based on rhodamine-6G have been designed and synthesized. XQN showed fluorescent specific selectivity and high sensitivity for Zn2+ against other metal ions such as Fe3+, Cr3+, Hg2+, Ag+, Ca2+, Cu2+, Pb2+, Cd2+, Ni2+, Co2+ and Mg2+ in CH3CN-PBS(phosphate buffer saline) (10 mM, v/v=7:3, pH = 7.4) solution. The distinct color change and the rapid emergence of fluorescence emission provided naked-eyes detection for Zn2+. The test strip results showed that these sensors could act as a convenient and efficient Zn2+ test kit. The recognition mechanism of the sensor toward Zn2+ was evaluated by IR and the Job’s plots. The detection limits of XQN towards Zn2+ was calculated as 2.39 and#181;M. In addition, XQN-Zn2+ fluorescence lifetime and fluorescence quantum yield were also measured.

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Enhanced fluorescence emission from rhodamine 6G dye through polymerization-induced self-assembly

Journal of Photochemistry and Photobiology A Chemistry ◽

10.1016/j.jphotochem.2020.112992 ◽

2021 ◽

Vol 406 ◽

pp. 112992

Author(s):

Chenyu Lin ◽

Sai Krishna Katla ◽

Juan Perez-Mercader

Keyword(s):

Self Assembly ◽

Rhodamine 6G ◽

Fluorescence Emission ◽

Enhanced Fluorescence ◽

Rhodamine 6G Dye

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Encapsulating toxic Rhodamine 6G dye, and Cr (VI) metal ions from liquid phase using AlPO4-5 molecular sieves. Preparation, characterization, and adsorption parameters

Journal of Molecular Liquids ◽

10.1016/j.molliq.2021.116549 ◽

2021 ◽

pp. 116549

Author(s):

Anjani R.K. Gollakota ◽

Venkata Subbaiah Munagapati ◽

Krishna Prasad Shadangi ◽

Guda Mallikarjuna Reddy ◽

Jet-Chau Wen ◽

...

Keyword(s):

Liquid Phase ◽

Metal Ions ◽

Molecular Sieves ◽

Rhodamine 6G ◽

Adsorption Parameters ◽

Rhodamine 6G Dye

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A novel method for visualizing and tracking endogenous mRNA in a specific cell population in pathological neovascularization

Scientific Reports ◽

10.1038/s41598-021-81367-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Md Imam Uddin ◽

Tyler C. Kilburn ◽

Sara Z. Jamal ◽

Craig L. Duvall ◽

John S. Penn

Keyword(s):

Real Time ◽

Disease Burden ◽

Ex Vivo ◽

Expression Patterns ◽

Fluorescence Emission ◽

High Sensitivity ◽

Living Systems ◽

Specific Cell ◽

Potential Marker

AbstractDiabetic retinopathy, retinopathy of prematurity and retinal vein occlusion are potentially blinding conditions largely due to their respective neovascular components. The development of real-time in vivo molecular imaging methods, to assess levels of retinal neovascularization (NV), would greatly benefit patients afflicted with these conditions. mRNA hybridization techniques offer a potential method to image retinal NV. The success of these techniques hinges on the selection of a target mRNA whose tissue levels and spatial expression patterns correlate closely with disease burden. Using a model of oxygen-induced retinopathy (OIR), we previously observed dramatic increases in retinal endoglin that localized to neovascular structures (NV), directly correlating with levels of neovascular pathology. Based on these findings, we have investigated Endoglin mRNA as a potential marker for imaging retinal NV in OIR mice. Also of critical importance, is the application of innovative technologies capable of detecting mRNAs in living systems with high sensitivity and specificity. To detect and visualize endoglin mRNA in OIR mice, we have designed and synthesized a novel imaging probe composed of short-hairpin anti-sense (AS) endoglin RNA coupled to a fluorophore and black hole quencher (AS-Eng shRNA). This assembly allows highly sensitive fluorescence emission upon hybridization of the AS-Eng shRNA to cellular endoglin mRNA. The AS-Eng shRNA is further conjugated to a diacyl-lipid (AS-Eng shRNA–lipid referred to as probe). The lipid moiety binds to serum albumin facilitating enhanced systemic circulation of the probe. OIR mice received intraperitoneal injections of AS-Eng shRNA–lipid. Ex vivo imaging of their retinas revealed specific endoglin mRNA dependent fluorescence superimposed on neovascular structures. Room air mice receiving AS-Eng shRNA–lipid and OIR mice receiving a non-sense control probe showed little fluorescence activity. In addition, we found that cells in neovascular lesions labelled with endoglin mRNA dependent fluorescence, co-labelled with the macrophage/microglia-associated marker IBA1. Others have shown that cells expressing macrophage/microglia markers associate with retinal neovascular structures in proportion to disease burden. Hence we propose that our probe may be used to image and to estimate the levels of retinal neovascular disease in real-time in living systems.

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