Anticancer activity of recombinant Siva1 protein in human nasopharyngeal carcinoma cell line CNE-2

2015 ◽  
Vol 15 (6) ◽  
pp. 833-841
Author(s):  
Guo-Hui Chen ◽  
Qian-Qian Xue ◽  
Jun Li ◽  
Tian-Le Gao ◽  
Qing-Shun Sun ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Anticancer Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Nasopharyngeal Carcinoma Cell ◽  
Human Nasopharyngeal Carcinoma

Salinomycin radiosensitizes human nasopharyngeal carcinoma cell line CNE-2 to radiation

Tumor Biology ◽  
2015 ◽  
Vol 37 (1) ◽  
pp. 305-311 ◽  
Author(s):  
Yongqin Zhang ◽  
Yun Zuo ◽  
Zhifeng Guan ◽  
Weidong Lu ◽  
Zheng Xu ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Nasopharyngeal Carcinoma Cell ◽  
Human Nasopharyngeal Carcinoma

Inhibition Human Nasopharyngeal Carcinoma Cells TNF-α Gene Transfected Cytokine-Induced Killer Cells Based on Nanomaterial Polyphthalamide Monoamine Dendrimer Nanomaterial

2020 ◽  
Vol 20 (10) ◽  
pp. 6133-6139
Author(s):  
Xiao Han ◽  
Xiaohui Wang ◽  
Li Wang ◽  
Xianyi Yao ◽  
Guangyu Zhao ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cell Line ◽  
Recombinant Plasmid ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Killer Cells ◽  
Cik Cells ◽  
Nasopharyngeal Carcinoma Cell ◽  
Human Nasopharyngeal Carcinoma ◽  
Cik Cell

The present study aims to investigate the possibility of TNF-α gene transfection into CIK (cytokine-induced killer) cells using the nanomaterial PAMAM and the inhibitory effects of these cells on the growth of the human nasopharyngeal carcinoma cell line CNE-2. The pEGFP-N1-TNF-α recombinant plasmid was constructed and used to transfect the CIK cells using the nanomaterial PAMAM. Subsequently, the transfection efficiency was measured. The ELISA method was used to analyze the CIK cell culture supernatant. TNF-α concentration in fluid, and CIK cell phenotype was analyzed by the flow cytometry. The MTT assay was used to detect the inhibitory activity of CIK cells on the growth of nasopharyngeal carcinoma cell line CNE-2 after transfection. The CIK cells were transfected with the nanomaterial PAMAM using the successfully constructed recombinant plasmid pEGFP-N1-TNF-α. The growth characteristics and phenotypic characteristics of the transfected CIK cells were not changed, and an increase in the TNF-α secretion was observed, indicating that the CIK cells can significantly inhibit CNE-2 cell growth (P < 0005).

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