1H NMR characterization of complexation of Glutathione with silver and aluminum metals in aqueous solution

Metallo-elements have both pharmacological and toxic effects on plants, animals and humans. These are considered as a major public health issue worldwide. In particular, heavy metals such as silver (Ag) and aluminum (Al) are environmentally widespread, and their relative toxicity can lead to numerous pathologies such as nephropathy, cancers, vascular and skin diseases. The goal of this study was to examine the behavioral effects of Ag and Al salts (i.e. Silver nitrate and Aluminum sulfate) on glutathione (GSH), a potent oxidant in biological mixtures. We also aimed to suggest mechanisms of action of thiolate complexed to these metallo-elements in competitive studies with Ellman’s reagent (5,5’-dithiobis(2-nitrobenzoic acid aka ESSE). By proton nuclear magnetic resonance (NMR) spectroscopy, detailed titrations were carried out for these metal thiols interactions in the presence of ESSE in order to elucidate first equilibrium and possible second equilibrium. We found by 1H NMR spectroscopy that GSH binds to Ag and Al, which highlighted possible in-vivo chelation mechanisms of GSH toward these toxic metallo-elements.

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Synthesis and 31 P NMR characterization of new low toxic highly sensitive pH probes designed for in vivo acidic pH studies

Bioorganic & Medicinal Chemistry ◽

10.1016/s0968-0896(01)00414-x ◽

2002 ◽

Vol 10 (5) ◽

pp. 1451-1458 ◽

Cited By ~ 16

Author(s):

Sophie Martel ◽

Jean-Louis Clément ◽

Agnès Muller ◽

Marcel Culcasi ◽

Sylvia Pietri

Keyword(s):

Acidic Ph ◽

Highly Sensitive ◽

Nmr Characterization ◽

Low Toxic ◽

Ph Probes

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Combined High-Resolution Solid-State 1H/13C NMR Spectroscopy and 1H NMR Relaxometry for the Characterization of Kerogen Thermal Maturation

Energy & Fuels ◽

10.1021/acs.energyfuels.0c02713 ◽

2020 ◽

Author(s):

Francesco Panattoni ◽

Jonathan Mitchell ◽

Edmund J. Fordham ◽

Ravinath Kausik ◽

Clare P. Grey ◽

...

Keyword(s):

Solid State ◽

Nmr Spectroscopy ◽

High Resolution ◽

1H Nmr ◽

13C Nmr ◽

13C Nmr Spectroscopy ◽

Thermal Maturation ◽

Nmr Relaxometry ◽

1H Nmr Relaxometry

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ISOLATION OF 2-CHLOROBENZIMIDAZOLE FROM MELIA DUBIA LEAF EXTRACT AND ITS STRUCTURAL CHARACTERISATION

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i10.20573 ◽

2017 ◽

Vol 9 (10) ◽

pp. 67 ◽

Cited By ~ 2

Author(s):

G. Dayana Jeyaleela ◽

S. Irudaya Monisha ◽

J. Rosaline Vimala ◽

A. Anitha Immaculate

Keyword(s):

Mass Spectrometry ◽

1H Nmr ◽

Research Work ◽

Proton Nuclear Magnetic Resonance ◽

Resonance Spectroscopy ◽

Ft Ir ◽

Uv Visible ◽

Isolated Compound ◽

Promising Source

Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubaiMethods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound.Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), Gas chromatography-mass spectrometry (GC-MS), and Mass spectrometry(MS). Based on the studies, organizational characteristics of one bioactive principle were deciphered. The results revealed that the isolated species is 2-chlorobenzimidazole and it agreed well with the reported value and spectra for 2-chlorobenzimidazole.Conclusion: The above results obtained in this research work clearly indicated the promising occurrence of 2-chlorobenzimidazole in Media dubia plant leaves. The future scope of these studies may guide us to view the biological activity of the isolated compound.

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