In-vitro Effects of Bacterial Melanin in Macrophage “RAW 264.7” Cell Culture

2020 ◽  
Vol 7 (3-4) ◽  
pp. 199-206
Author(s):  
Tigran Petrosyan ◽  
Anichka Hovsepyan ◽  
Sona Avetisyan ◽  
Noble Kurian
Keyword(s):  
Cell Culture ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Bacterial Melanin ◽  
In Vitro Effects

Branched chain amino Acids as in vitro and in vivo Anti-Oxidation Compounds

2021 ◽  
pp. 3899-3904
Author(s):  
Moath Alqaraleh ◽  
Violet Kasabri ◽  
Ibrahim Al-Majali ◽  
Nihad Al-Othman ◽  
Nihad Al-Othman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Amino Acids ◽  
Branched Chain Amino Acids ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Branched Chain ◽  
Raw 264.7 Cell Line

Background and aims: Branched chain amino acids (BCAAs) can be tightly connected to metabolism syndrome (MetS) which can be counted as a metabolic indicator in the case of insulin resistance (IR). The aim of this study was to assess the potential role of these acids under oxidative stress. Material and Methods: the in vitro antioxidant activity of BCAAs was assessed using free radical 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging assays. For further check, a qRT-PCR technique was madefor detection the extent of alterations in gene expression of antioxidative enzymes (catalase and glutathione peroxidase (Gpx)) in lipopolysaccharides (LPS(-induced macrophages RAW 264.7 cell line. Additionally, BCAAs antioxidant activity was evaluated based on plasma H2O2 levels and xanthine oxidase (XO) activity in prooxidative LPS-treated mice. Results: Different concentrations of BCAAs affected on DPPH radical scavenging activity but to lesser extent than the ascorbic acid. Besides, BCAAs obviously upregulated the gene expression levels of catalases and Gpx in LPS-modulated macrophage RAW 264.7 cell line. In vivo BCAAs significantly minimized the level of plasma H2O2 as well as the activity of XO activity under oxidative stress. Conclusion: our current findings suggest that BCAAs supplementation may potentially serve as a therapeutic target for treatment of oxidative stress occurs with atherosclerosis, IR-diabetes, MetS and tumorigenesis.

scholarly journals Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation

2013 ◽  
Vol 35 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Long-gang He ◽  
Xiang-lian Li ◽  
Xiang-zhou Zeng ◽  
Heng Duan ◽  
Song Wang ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Raw 264.7 ◽  
Raw 264.7 Cell

scholarly journals Anti-inflammatory flavonol acylglycosides from the aerial part of Lindera akoensis Hayata

RSC Advances ◽  
2017 ◽  
Vol 7 (80) ◽  
pp. 50868-50874 ◽  
Author(s):  
Hui-Chi Huang ◽  
Chung-Ping Yang ◽  
Sheng-Yang Wang ◽  
Chi-I Chang ◽  
Ping-Jyun Sung ◽  
...  
Keyword(s):  
Aerial Part ◽  
Inflammatory Activity ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Anti Inflammatory ◽  
Activity Decrease

Flavonol acylglycosides, linderakosides A–E (1–5) were characterized from L. akoensis. Compounds 1, 2, and 5 exhibited showed in vitro anti-inflammatory activity decrease the LPS-stimulated production of nitrite in RAW 264.7 cell.

scholarly journals In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line

2020 ◽  
Vol 71 (2) ◽  
pp. 443-448
Author(s):  
Dora Domnica Baciu ◽  
Aurora Salageanu ◽  
Teodor Visan
Keyword(s):  
Monosodium Glutamate ◽  
Food Additive ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Cell Mortality ◽  
Elisa Technique ◽  
Proinflammatory Activity ◽  
Raw 264.7 Cell Line

Monosodium glutamate (E-621, abbreviated MSG) is a food additive widely used in the food domain as a flavor and taste enhancer. The present study aims to evaluate in vitro the effects of increased concentrations of MSG, using the RAW 264.7 murine macrophage line. The study was conducted in 3 complementary directions: first, establishing the ability of monosodium glutamate to induce cell mortality, by using the MTT assay to determine cell viability; second, establishing by its oxidizing activity if MSG is toxic for cells inducing oxidative stress, measured by the Griess test for determination of extracellular NO; third, to observe whether MSG presence induces an immune response quantified by the secretion of proinflammatory cytokines, TNFα in this case, measured through the immunoenzymatic ELISA technique.

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