In-vitro Evaluation of Immune Enhancing Potential of Novel siddha formulation Rasa Chendhuram using Murine Macrophage RAW 264.7 Cell Line Model

The poxviruses are large, linear, double-stranded DNA viruses about 130 to 230 kbp, that have an animal origin and evolved to infect a wide host range. Variola virus (VARV), the causative agent of smallpox, is a poxvirus that infects only humans, but other poxviruses such as monkey poxvirus and cowpox virus (CPXV) have crossed over from animals to infect humans. Therefore understanding the biology of poxviruses can devise antiviral strategies to prevent these human infections. In this study we used a system-based approach to examine the host responses to three orthopoxviruses, CPXV, vaccinia virus (VACV), and ectromelia virus (ECTV) in the murine macrophage RAW 264.7 cell line. Overall, we observed a significant down-regulation of gene expressions for pro-inflammatory cytokines, chemokines, and related receptors. There were also common and virus-specific changes in the immune-regulated gene expressions for each poxvirus-infected RAW cells. Collectively our results showed that the murine macrophage RAW 264.7 cell line is a suitable cell-based model system to study poxvirus host response.

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l-arginine-dependent nitric oxide production of a murine macrophage-like RAW 264.7 cell line stimulated with Porphyromonas gingivalis lipopolysaccharide

Oral Microbiology and Immunology ◽

10.1046/j.0902-0055.2003.00108.x ◽

2004 ◽

Vol 19 (2) ◽

pp. 65-70 ◽

Cited By ~ 16

Author(s):

W. Sosroseno ◽

E. Herminajeng ◽

P. S. Bird ◽

G. J. Seymour

Keyword(s):

Nitric Oxide ◽

Cell Line ◽

Porphyromonas Gingivalis ◽

Raw 264.7 ◽

Nitric Oxide Production ◽

Murine Macrophage ◽

Raw 264.7 Cell ◽

Oxide Production ◽

Porphyromonas Gingivalis Lipopolysaccharide ◽

Raw 264.7 Cell Line

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Branched chain amino Acids as in vitro and in vivo Anti-Oxidation Compounds

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00677 ◽

2021 ◽

pp. 3899-3904

Author(s):

Moath Alqaraleh ◽

Violet Kasabri ◽

Ibrahim Al-Majali ◽

Nihad Al-Othman ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Amino Acids ◽

Branched Chain Amino Acids ◽

Raw 264.7 ◽

Raw 264.7 Cell ◽

Branched Chain ◽

Raw 264.7 Cell Line

Background and aims: Branched chain amino acids (BCAAs) can be tightly connected to metabolism syndrome (MetS) which can be counted as a metabolic indicator in the case of insulin resistance (IR). The aim of this study was to assess the potential role of these acids under oxidative stress. Material and Methods: the in vitro antioxidant activity of BCAAs was assessed using free radical 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging assays. For further check, a qRT-PCR technique was madefor detection the extent of alterations in gene expression of antioxidative enzymes (catalase and glutathione peroxidase (Gpx)) in lipopolysaccharides (LPS(-induced macrophages RAW 264.7 cell line. Additionally, BCAAs antioxidant activity was evaluated based on plasma H2O2 levels and xanthine oxidase (XO) activity in prooxidative LPS-treated mice. Results: Different concentrations of BCAAs affected on DPPH radical scavenging activity but to lesser extent than the ascorbic acid. Besides, BCAAs obviously upregulated the gene expression levels of catalases and Gpx in LPS-modulated macrophage RAW 264.7 cell line. In vivo BCAAs significantly minimized the level of plasma H2O2 as well as the activity of XO activity under oxidative stress. Conclusion: our current findings suggest that BCAAs supplementation may potentially serve as a therapeutic target for treatment of oxidative stress occurs with atherosclerosis, IR-diabetes, MetS and tumorigenesis.

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