scholarly journals Identification and quantitative determination of the flavonoids of the complex dense extract of st. john's wort herb and pot marigold flowers

2018 ◽  
pp. 71-79
Author(s):  
T. A. Shostak ◽  
T. G. Kalyniuk ◽  
L.V. Vronska
Keyword(s):  
Active Pharmaceutical Ingredient ◽  
Biologically Active ◽  
Chromatography Method ◽  
St John’S Wort ◽  
St John's Wort ◽  
Chromatographic Profile ◽  
Pot Marigold ◽  
Layer Chromatography

Common Saint-John's wort (Hypericum perforatum) and pot marigold (Calendula officinalis) are rich in such biologically active substances (BAS) as carotene, ascorbic acid, essential oils, vitamins, tannin and resinous substances, as well as flavonoids that bear evident wound healing properties and antiulcerous properties. The object of this study was BAR composition of the complex dense herb extract of St. John's wort and flowers of marigolds (1:10). In order to introduce a new herbal substance into medical practice, it is necessary to develop methods for its identification and quantification. The TLC [thin layer chromatography] method was used to identify the BAR in the extract under study, and the method of absorption spectrophotometry was proposed for quantification of the content of flavonoids. As a result of the conducted research, there were selected characteristic substances - identification markers of the extract, the choice of which was in accordance with the requirements of the SPF on the quality of the herb of St. John's wort and the flowers of pot marigold, and there was indicated the position and coloring of the zones in the chromatographic profile of the tested extract solution. Such approach will enable objective identification of the extract as a substance and as an active pharmaceutical ingredient in the formulation. The criterion for quantitative standardization of the complex dense extract is the content of the amount of flavonoids not less than 1.5% in terms of hyperoside and dry substance.

Extraction and determination of biologically active components of St. John’s wort and its pharmaceutical preparations

2016 ◽  
Vol 71 (7) ◽  
pp. 741-747 ◽  
Author(s):  
V. V. Milevskaya ◽  
M. A. Statkus ◽  
Z. A. Temerdashev ◽  
N. V. Kiseleva ◽  
T. S. Butyl’skaya ◽  
...  
Keyword(s):  
Pharmaceutical Preparations ◽  
Biologically Active ◽  
Active Components ◽  
St John’S Wort ◽  
St John's Wort

Determination of Hypericin in St. John's Wort (Hypericum perforatum L.) extracts using HPLC-ED

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
E Sofic ◽  
A Copra Janicijevic ◽  
M Maksimovic ◽  
I Tahirovic ◽  
L Klepo ◽  
...  
Keyword(s):  
Hypericum Perforatum ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

scholarly journals Application of the thin-layer chromatography method for analysis of plant antioxidant activity

Food systems ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 26-30
Author(s):  
N. V. Kupaeva ◽  
E. R. Vasilevskaya ◽  
L. V. Fedulova ◽  
E. A. Kotenkova
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Chromatography Method ◽  
Physicochemical Methods ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Active Substances

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.

scholarly journals Assessment of the quality of peppermint leaves by thin-layer chromatography in some objects of plant origin

2020 ◽  
Vol 19 (5-6) ◽  
pp. 148-154
Author(s):  
Vali A. Sahratov ◽  
Tamara L. Malkova ◽  
Ludmila N. Karpova ◽  
Anna A. Pospelova
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Biologically Active ◽  
Mentha Piperita ◽  
Plant Origin ◽  
Plant Materials ◽  
Layer Chromatography ◽  
Active Substances

The State Pharmacopoeia of the XIV edition defines the approach for the assessment of the quality of medicinal plant materials, it deals with the identification of the main groups of biologically active substances by thin layer chromatography. According to this approach, the analysis of some types of medicinal plant materials as a part of some objects of plant origin was carried out. The article presents the quality assessment algorithm as examplified by peppermint leaves (Mentha piperita L.), which are part of a variety of plant object.

Quality and yield in St. John's wort (Hypericum perforatum L.) harvested in different phenological stages

2002 ◽  
Vol 50 (3) ◽  
pp. 349-358 ◽  
Author(s):  
F. Hevia ◽  
M. Berti ◽  
R. Wilckens
Keyword(s):  
Hypericum Perforatum ◽  
Block Design ◽  
Flower Bud ◽  
Phenological Stages ◽  
St John’S Wort ◽  
St John's Wort ◽  
Yield And Quality ◽  
Hypericum Perforatum L ◽  
Beginning Of Flowering

Two experiments were conducted in the province of Ñuble, Chile during the 1997/98 and 1998/99 seasons with the objective of evaluating the effect of harvesting date on the yield and quality of St. John's wort (Hypericum perforatum L.) in the second year of production. The apical 25 cm of the stem were harvested in the following stages: flower bud, beginning of flowering, full flower and petal drop. A randomized complete block design with four replications was used. The best yield (fresh, dry and threshing weight) and the highest hypericin content were obtained at the petal drop stage. Nevertheless, the results indicate that the best time to harvest St. John's wort is when 10 to 20% of the flowers are open and the rest are in the bud stage.

scholarly journals Determination of St. John's Wort Components in Dietary Supplements and Functional Foods by Liquid Chromatography

2002 ◽  
Vol 85 (6) ◽  
pp. 1360-1369 ◽  
Author(s):  
Catharina Y W Ang ◽  
Yanyan Cui ◽  
Hebron C Chang ◽  
Wenhong Luo ◽  
Thomas M Heinze ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Dietary Supplement ◽  
Functional Foods ◽  
Reversed Phase ◽  
Laboratory Method ◽  
St John’S Wort ◽  
St John's Wort ◽  
Photodiode Array ◽  
Liquid Chromatographic

Abstract St. John's wort ( Hypericum perforatum L.) preparations, a top-selling botanical dietary supplement used primarily as an antidepressant, has recently been used as an ingredient in some food products sold as functional foods. A rapid extraction technique followed by a liquid chromatographic (LC) method was developed to determine 4 characteristic bioactive compounds (pseudohypericin, hypericin, hyperforin, and adhyperforin) from St. John's wort in dietary supplements and functional foods to which it was added. Solid samples, including including dried leaf/flower mixture, dietary supplement capsules, tea bags, puff and snack bar, were extracted with methanol by sonication.Noncarbonated, fruit-flavored drinks were centrifuged and mixed with methanol. Compounds were then determined by isocratic, reversed-phase LC with UV detection at 2 wavelengths and further identified or confirmed by photodiode array spectra and LC/mass spectrometry. Within-laboratory method variations (% RSD) were satisfactory. Very low amounts, if any, of the 4 components were found in drink and puff samples, and none was found in the snack bar. The methods developed provide a useful means for the determination of St. John's wort components in dietary supplements and functional foods.

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