scholarly journals Triiodothyronine Uptake Measurement

2020 ◽  
Author(s):  
Keyword(s):  

2021 ◽  
Vol 22 (7) ◽  
pp. 3691
Author(s):  
Oliver Schmutzler ◽  
Sebastian Graf ◽  
Nils Behm ◽  
Wael Y. Mansour ◽  
Florian Blumendorf ◽  
...  

Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.





2018 ◽  
Vol 76 (6) ◽  
pp. 1757-1759 ◽  
Author(s):  
Ngoc Nguyen ◽  
Barbara Ozarska ◽  
Macarthur Fergusson ◽  
Peter Vinden


Author(s):  
Surendra K. Shukla ◽  
Scott E. Mulder ◽  
Pankaj K. Singh


2005 ◽  
Vol 64 (3) ◽  
pp. 207-215 ◽  
Author(s):  
Chenhui Zou ◽  
Yajie Wang ◽  
Zhufang Shen




1976 ◽  
Vol 11 (5) ◽  
pp. 414
Author(s):  
Arthur Z Filiatraut ◽  
Marc Tetalman ◽  
Jerome Dare ◽  
John Scheu ◽  
Bobby Gray ◽  
...  


1985 ◽  
Vol 249 (6) ◽  
pp. H1211-H1215
Author(s):  
J. J. Murray ◽  
A. V. Kuzmin ◽  
P. W. Reed ◽  
D. O. Levitsky

The divalent cation ionophore A23187 at a concentration of 1 nM produced an increased rate of oxalate-supported calcium uptake by isolated cardiac sarcoplasmic reticulum as determined by absorbance changes of the calcium-sensitive dye murexide. Addition of a higher concentration of A23187 (0.1 microM) produced a decreased rate of calcium uptake. Measurement of the time during which ATPase was activated by calcium addition also suggested an increased rate of calcium uptake in the presence of 1 nM A23187 and an inhibition of calcium uptake at a higher concentration of the ionophore (0.1 microM). Ca2+-stimulated ATPase activity and incorporation of 32Pi from [gamma-32P]ATP into sarcoplasmic reticular proteins were increased by A23187 at concentrations of 1 nM or greater. An increased coupling of calcium uptake to ATP hydrolysis was observed at 1 nM A23187, while concentrations of the ionophore greater than or equal to 10 nM produced a decreased coupling. Addition of an inhibitor of cyclic AMP-dependent protein kinase decreased the rate of calcium uptake, and this inhibition was reversed in a concentration-dependent manner by 0.01–1 nM A23187. These data suggest that A23187 can activate a mechanism involving the calcium-dependent phosphorylation of protein that may regulate the activity of the calcium uptake system of the sarcoplasmic reticulum. These observations appear to provide an explanation for some of the contractile effects of A23187 in intact cardiac muscle that suggest that treatment with the ionophore results in an increased sequestration of calcium from the cytoplasm.



1965 ◽  
Vol 20 (4) ◽  
pp. 621-627 ◽  
Author(s):  
William Perl ◽  
Herbert Rackow ◽  
Ernest Salanitre ◽  
Gerald L. Wolf ◽  
Robert M. Epstein

An approximately constant 5% difference in alveolar concentration of nitrous oxide and cyclopropane exists when these two gases are administered simultaneously to human subjects. This difference in uptake cannot be fully explained within the traditional framework of a perfusion-limited, multi-compartment model of inert gas exchange. It is proposed that this difference reflects direct diffusion from lean to neighboring adipose tissue through distances of the order of 1 mm. The diffusional rate of cyclopropane uptake into adipose tissue is initially large relative to perfusional uptake. The two rates eventually become and remain comparable as both decrease to zero. Implications of these results for deduction of blood flow to body adipose tissue by gas uptake measurement, and for utilization of capillary exchange surface by fat-soluble gases in adipose tissue are discussed. compartment model generalization; gas uptake in body; inert, fat-soluble gas uptake; kinetics of gas exchange in body; body uptake of inert gases; fat-soluble gas uptake; distribution kinetics of gases in body Submitted on February 3, 1964



1986 ◽  
Vol 95 (2) ◽  
pp. 200-204 ◽  
Author(s):  
James C. Denneny ◽  
Robert A. Weisman ◽  
David G. Silverman ◽  
David A. Brousseau

The fluorescein test has been used as a means of assessing flap viability for almost 40 years. This study was performed in an attempt to determine if recording of the elirnination of dye—combined with dye uptake measurement—could provide reliable and consistent monitoring of island flap perfusion.



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