scholarly journals Multiplex Amplifiable Probe Hybridization

2020 ◽  
Author(s):  
Keyword(s):  
Probe Hybridization

Detection of enteric adenoviruses in south African waters using gene probes

1995 ◽  
Vol 31 (5-6) ◽  
pp. 345-350 ◽  
Author(s):  
B. Genthe ◽  
M. Gericke ◽  
B. Bateman ◽  
N. Mjoli ◽  
R. Kfir
Keyword(s):  
South African ◽  
Water Samples ◽  
Cellulose Fibre ◽  
Gene Probe ◽  
Viral Dna ◽  
Virus Particles ◽  
Gene Probes ◽  
Probe Hybridization ◽  
Enteric Adenoviruses ◽  
Effect Of Chlorine

Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultrafiltration and analysed directly for the presence of enteric adenoviruses. Three pretreatment techniques, namely sephadex columns, cellulose fibre and GenecleanTM were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed. Cellulose fibre and GenecleanTM were found to successfully remove inhibitory substances from concentrated raw waters. Viral DNA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.

scholarly journals Simultaneous oligonucleotide probe hybridization and immunostaining for in situ detection of Gordona species in activated sludge

1999 ◽  
Vol 29 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Daniel B Oerther ◽  
Francis L Reyes ◽  
Mark Hernandez ◽  
Lutgarde Raskin
Keyword(s):  
Activated Sludge ◽  
Oligonucleotide Probe ◽  
Probe Hybridization ◽  
In Situ Detection

scholarly journals por Variable-Region Typing by DNA Probe Hybridization Is Broadly Applicable to Epidemiologic Studies of Neisseria gonorrhoeae

2005 ◽  
Vol 43 (4) ◽  
pp. 1522-1530 ◽  
Author(s):  
M. C. Bash ◽  
P. Zhu ◽  
S. Gulati ◽  
D. McKnew ◽  
P. A. Rice ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Variable Region ◽  
Epidemiologic Studies ◽  
Dna Probe ◽  
Probe Hybridization

scholarly journals Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes

2006 ◽  
Vol 72 (8) ◽  
pp. 5311-5317 ◽  
Author(s):  
Kengo Kubota ◽  
Akiyoshi Ohashi ◽  
Hiroyuki Imachi ◽  
Hideki Harada
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence Intensity ◽  
Dna Probes ◽  
Locked Nucleic Acid ◽  
Factors Affecting ◽  
Hybridization Efficiency ◽  
Probe Hybridization ◽  
Major Factors

ABSTRACT Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. Fluorescently labeled LNA/DNA probes with two to four LNA substitutions exhibited strong fluorescence intensities equal to or greater than that of probe Eub338, although these probes did not show bright signals when they were synthesized as DNA probes; for example, the fluorescence intensity of probe Eco468 increased by 22-fold after three LNA bases were substituted for DNA bases. Dissociation profiles of the probes revealed that the dissociation temperature was directly related to the number of LNA substitutions and the fluorescence intensity. These results suggest that the introduction of LNA residues in DNA probes will be a useful approach for effectively enhancing probe hybridization efficiency.

scholarly journals Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip

2015 ◽  
Vol 14 (3) ◽  
pp. 9298-9305
Author(s):  
M.Z. Zhang ◽  
X.F. Zhang ◽  
X.M. Chen ◽  
X. Chen ◽  
S. Wu ◽  
...  
Keyword(s):  
Genetic Modification ◽  
Specific Detection ◽  
Probe Hybridization

scholarly journals Genotyping of group A rotavirus samples from Brazilian children by probe hybridization

2001 ◽  
Vol 34 (4) ◽  
pp. 471-473 ◽  
Author(s):  
D.D.P. Cardoso ◽  
M.L. Rácz ◽  
M.S.P. Azevedo ◽  
R.M.B. Martins ◽  
C.M.A. Soares
Keyword(s):  
Group A Rotavirus ◽  
Group A ◽  
Probe Hybridization ◽  
Brazilian Children

scholarly journals Prochlorococcus Ecotype Abundances in the North Atlantic Ocean As Revealed by an Improved Quantitative PCR Method

2006 ◽  
Vol 72 (1) ◽  
pp. 723-732 ◽  
Author(s):  
Erik R. Zinser ◽  
Allison Coe ◽  
Zackary I. Johnson ◽  
Adam C. Martiny ◽  
Nicholas J. Fuller ◽  
...  
Keyword(s):  
Atlantic Ocean ◽  
Quantitative Pcr ◽  
North Atlantic ◽  
North Atlantic Ocean ◽  
Culture Collections ◽  
The North ◽  
Field Samples ◽  
Pcr Method ◽  
Probe Hybridization ◽  
Eastern North Atlantic

ABSTRACT The cyanobacterium Prochlorococcus numerically dominates the photosynthetic community in the tropical and subtropical regions of the world's oceans. Six evolutionary lineages of Prochlorococcus have been described, and their distinctive physiologies and genomes indicate that these lineages are “ecotypes” and should have different oceanic distributions. Two methods recently developed to quantify these ecotypes in the field, probe hybridization and quantitative PCR (QPCR), have shown that this is indeed the case. To facilitate a global investigation of these ecotypes, we modified our QPCR protocol to significantly increase its speed, sensitivity, and accessibility and validated the method in the western and eastern North Atlantic Ocean. We showed that all six ecotypes had distinct distributions that varied with depth and location, and, with the exception of the deeper waters at the western North Atlantic site, the total Prochlorococcus counts determined by QPCR matched the total counts measured by flow cytometry. Clone library analyses of the deeper western North Atlantic waters revealed ecotypes that are not represented in the culture collections with which the QPCR primers were designed, explaining this discrepancy. Finally, similar patterns of relative ecotype abundance were obtained in QPCR and probe hybridization analyses of the same field samples, which could allow comparisons between studies.

scholarly journals Analysis of Human Mitochondrial DNA Content by Southern Blotting and Nonradioactive Probe Hybridization

2019 ◽  
Vol 80 (1) ◽  
Author(s):  
Joel H. Wheeler ◽  
Carolyn K. J. Young ◽  
Matthew J. Young
Keyword(s):  
Mitochondrial Dna ◽  
Dna Content ◽  
Southern Blotting ◽  
Human Mitochondrial Dna ◽  
Mitochondrial Dna Content ◽  
Probe Hybridization

scholarly journals Discovery of copy number variants by multiplex amplifiable probe hybridization (MAPH) in candidate pigmentation genes

2015 ◽  
Vol 42 (5) ◽  
pp. 485-493 ◽  
Author(s):  
Saioa López ◽  
Iker García ◽  
Isabel Smith ◽  
Arrate Sevilla ◽  
Neskuts Izagirre ◽  
...  
Keyword(s):  
Copy Number ◽  
Copy Number Variants ◽  
Probe Hybridization
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