Growth/Differentiation Factor 9

2020 ◽  
Author(s):  
Keyword(s):  
Differentiation Factor ◽  
Growth Differentiation Factor 9

scholarly journals A 5-Methylcytosine Site of Growth Differentiation Factor 9 (GDF9) Gene Affects Its Tissue-Specific Expression in Sheep

Animals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 200
Author(s):  
Zhangyuan Pan ◽  
Xiangyu Wang ◽  
Ran Di ◽  
Qiuyue Liu ◽  
Wenping Hu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Methylation Level ◽  
Cpg Island ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Directed Mutation ◽  
Differentiation Factor ◽  
Growth Differentiation Factor 9 ◽  
Basal Transcriptional Activity

Growth differentiation factor 9 (GDF9) plays an important role in the early folliculogenesis of sheep. This study investigated the mRNA expression of ovine GDF9 in different tissues by real-time PCR. GDF9 exhibits significantly higher levels of expression (p < 0.01) in the ovary, relative to other tissues, indicating that its expression is tissue specific. To explore the regulatory mechanism of this tissue-specific expression, the methylation level of one CpG island (−1453 to −1854) of GDF9 promoter in ovary and heart was determined. In this region (−1987 to −1750), only the mC-4 site was present in the Sp4 binding site showed differential methylation between the heart and ovary; with increased (p < 0.01) methylation being observed in the heart. Additionally, the methylation level was negatively correlated with GDF9 mRNA expression (R = −0.75, p = 0.012), indicating that the methylation of this site plays an important role in transcriptional regulation of GDF9. The methylation effect of the mC-4 site was confirmed by using dual-luciferase. Site-directed mutation (methylation) of mC-4 site significantly reduced (p < 0.05) basal transcriptional activity of GDF9 promoter in oocytes. These results imply that methylation of GDF9 promoter CpG island mC-4 site may affect the binding of the Sp4 transcription factor to the GDF9 promoter region in sheep, thereby regulating GDF9 expression and resulting in a tissue-specific expression.

Expression of growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and BMP receptors in the ovaries of goats

2004 ◽  
Vol 70 (1) ◽  
pp. 11-19 ◽  
Author(s):  
J.R.V. Silva ◽  
R. van den Hurk ◽  
H.T.A. van Tol ◽  
B.A.J. Roelen ◽  
J.R. Figueiredo
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bmp Receptors ◽  
Differentiation Factor ◽  
Morphogenetic Protein ◽  
Growth Differentiation Factor 9 ◽  
Bone Morphogenetic Protein 15

scholarly journals Corrigendum to “Polymorphisms in the Chicken Growth Differentiation Factor 9 Gene Associated with Reproductive Traits”

2018 ◽  
Vol 2018 ◽  
pp. 1-1 ◽  
Author(s):  
Lingbin Liu ◽  
Zhifu Cui ◽  
Qihai Xiao ◽  
Haihan Zhang ◽  
Xiaoling Zhao ◽  
...  
Keyword(s):  
Reproductive Traits ◽  
Differentiation Factor ◽  
Growth Differentiation Factor 9
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