scholarly journals Sialyl-Tn Antigen Antibody

2020 ◽  
Author(s):  
Keyword(s):  
Tn Antigen ◽  
Sialyl Tn Antigen ◽  
Antigen Antibody ◽  
Sialyl Tn

scholarly journals Predictive value of preoperative serum sialyl tn antigen levels in prognosis of patients with gastric cancer

Cancer ◽  
1993 ◽  
Vol 72 (6) ◽  
pp. 1836-1840 ◽  
Author(s):  
Ikuo Takahashi ◽  
Yoshihiko Maehara ◽  
Tetsuya Kusumoto ◽  
Motofumi Yoshida ◽  
Yoshihiro Kakeji ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Predictive Value ◽  
Tn Antigen ◽  
Preoperative Serum ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

20 SIALYLTRANSFERASE GENE EXPRESSION IN GGTA1 KNOCKOUT PIGS

2016 ◽  
Vol 28 (2) ◽  
pp. 140
Author(s):  
G. A. Kim ◽  
J.-X. Jin ◽  
S. Lee ◽  
A. Oh ◽  
B. C. Lee
Keyword(s):  
Gene Expression ◽  
Somatic Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Internal Standard ◽  
Immune Rejection ◽  
Tn Antigen ◽  
Transgenic Pigs ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

It is considered that GGTA1 knockout (KO) pig production via somatic cell NT would overcome the problem of immune rejection after xenotransplantation. It is reported that although GGTA KO mice showed only a mild increase in sialyltransferase gene expression, GGTA1 deficiency in pig could increase the sialyltransferase activities, non-Gal epitope expression, consequently may raise non-Gal xenoantigenicity. Therefore, in the present study we investigated whether the expression level of Sia-containing glycoconjugate mRNA in transgenic pigs could be affected by knocking out the GGTA1 gene. Besides GGTA1 KO pigs, double genes overexpressing pigs (2TG) and GGTA1 KO with double genes overexpressing (KO+2TG) pigs were produced by somatic cell NT. For the present study, fibroblasts were isolated from wild-type pigs without gene modification, 2TG, GGTA1 KO, and KO+2TG pig. The GAPDH gene was used as an internal standard to normalise the real-time PCR (RT-qPCR) analysis reaction efficiency and to quantify mRNA in pigs-derived fibroblast. The expression levels were compared between them (RT-qPCR) in triplicate for each sample. Oligonucleotide primers for real-time PCR were designed for Hanganutziu-Deicher antigen (ST3Gal1–4, ST6Gal1) and Sialyl-Tn antigen (ST6GalNac1, ST6GalNac2, and ST6GalNac6) analysis. For statistical analysis, one-way ANOVA with Dunn’s multiple comparison test were used. The mRNA expression of GGTA1 KO and KO+2TG pig derived fibroblasts cells genes showed that ST3Gal1, ST3Gal2, ST3Gal3, and ST6Gal1 gene expression were significantly up-regulated compared to the wild and 2TG pigs (P < 0.05). However, ST3Gal4, Sialyl-Tn antigen including ST6GalNac1, ST6GalNac2, and ST6GalNac6 in KO+2TG pigs were not different compared with the wild pigs (P > 0.05), whereas only GGTA1 KO pigs showed significantly higher expressions than wild, 2TG, and KO+2TG pigs (P < 0.05). These results demonstrated that GGTA KO pig-derived cells exhibit a higher Hanganutziu-Deicher antigen on glycoprotein and glycolipid than controls, and KO+2TG pig exhibit no differences when compared with GGTA1 KO pig, indicating that they do not act as an immune antigen in xenograft. Overall, the increase in glycosyltransferase expression suggests a corresponding increase in the cell surface sialyation in GGTA KO pig cells. For xenotransplantation, KO+2TG pigs were more preferable because of absence of immune rejection for Sia-containing glycoconjugate on glycoprotein and glycolipid than GGTA KO pigs. This study was supported by the Ministry of Trade, Industry and Energy (#10048948), Korea IPET (#114059–3), Research Institute for Veterinary Science, TS Corporation, and the BK21 plus program.

Different Levels of Sialyl-Tn Antigen Expressed on MUC16 in Patients With Endometriosis and Ovarian Cancer

2012 ◽  
Vol 22 (4) ◽  
pp. 531-538 ◽  
Author(s):  
Kaoru Akita ◽  
Shuhei Yoshida ◽  
Yuzuru Ikehara ◽  
Sayumi Shirakawa ◽  
Munetoyo Toda ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Tn Antigen ◽  
Sialyl Tn Antigen ◽  
Different Levels ◽  
Sialyl Tn

scholarly journals Colon carcinoma glycoproteins carrying α 2,6-linked sialic acid reactive withSambucus Nigra agglutinin are not constitutively expressed in normal human colon mucosa and are distinct from sialyl-tn antigen

1997 ◽  
Vol 70 (5) ◽  
pp. 575-581 ◽  
Author(s):  
Toshihiko Murayama ◽  
Christian Zuber ◽  
Walter K.F. Seelentag ◽  
Wei-Ping Li ◽  
Wolfgang Kemmner ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Colon Mucosa ◽  
Tn Antigen ◽  
Normal Human ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

Tissue Expression of Sialyl Tn Antigen in Gynecologic Tumors

1995 ◽  
Vol 21 (4) ◽  
pp. 385-389 ◽  
Author(s):  
Fumitaka Numa ◽  
Nagato Tsunaga ◽  
Toru Michioka ◽  
Shugo Nawata ◽  
Hidenobu Ogata ◽  
...  
Keyword(s):  
Tissue Expression ◽  
Tn Antigen ◽  
Gynecologic Tumors ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

High serum concentrations of sialyl Tn antigen in carcinomas of the biliary tract and pancreas

1999 ◽  
Vol 6 (4) ◽  
pp. 391-395 ◽  
Author(s):  
Atsushi Nanashima ◽  
Hiroyuki Yamaguchi ◽  
Tohru Nakagoe ◽  
Seiji Matsuo ◽  
Yorihisa Sumida ◽  
...  
Keyword(s):  
Biliary Tract ◽  
High Serum ◽  
Serum Concentrations ◽  
Tn Antigen ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

scholarly journals The interaction between Siglec-15 and tumor-associated sialyl-Tn antigen enhances TGF-  secretion from monocytes/macrophages through the DAP12-Syk pathway

Glycobiology ◽  
2012 ◽  
Vol 23 (2) ◽  
pp. 178-187 ◽  
Author(s):  
R. Takamiya ◽  
K. Ohtsubo ◽  
S. Takamatsu ◽  
N. Taniguchi ◽  
T. Angata
Keyword(s):  
Tn Antigen ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

Differences in sialyl-Tn antigen expression between keratoacanthomas and cutaneous squamous cell carcinomas

1999 ◽  
Vol 26 (4) ◽  
pp. 183-189 ◽  
Author(s):  
Petter Jensen ◽  
Ole P. F. Clausen ◽  
Magne Bryne
Keyword(s):  
Squamous Cell ◽  
Antigen Expression ◽  
Squamous Cell Carcinomas ◽  
Tn Antigen ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

Serum Sialyl-Tn Antigen Levels in Patients with Digestive Cancers

Oncology ◽  
1991 ◽  
Vol 48 (4) ◽  
pp. 321-326 ◽  
Author(s):  
Yoshiharu Motoo ◽  
Hiroyasu Kawakami ◽  
Hiroyuki Watanabe ◽  
Yoshitake Satomura ◽  
Hideki Ohta ◽  
...  
Keyword(s):  
Tn Antigen ◽  
Digestive Cancers ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn

Blood-Group Sialyl-Tn Antigen Is More Specific than Tn as a Tumor Marker in the Pancreas

Pancreas ◽  
1994 ◽  
Vol 9 (6) ◽  
pp. 698-702 ◽  
Author(s):  
C. K. Ching ◽  
S. W. Holmes ◽  
G. K. T. Holmes ◽  
R. G. Long
Keyword(s):  
Tumor Marker ◽  
Blood Group ◽  
Tn Antigen ◽  
Sialyl Tn Antigen ◽  
Sialyl Tn
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