Determination of histamine in fish and fish products: validation of photometric method

2021 ◽  
pp. 58-61
Author(s):  
O.O. Kharchenko ◽  
◽  
M.P. Hulich ◽  
O.V. Yashchenko ◽  
I.Ye. Moiseienko ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Photometric Method ◽  
Inexpensive Method ◽  
Total Uncertainty ◽  
Fish Products ◽  
High Concentrations

Topicality: The content of histamine in high concentrations causes a number of diseases that even lead to death, it is one of the indicators that characterizes the safety of fish and fish products. Therefore, the choice or development of a simple and inexpensive method for the determination of histamine content in fish and fish products is relevant. Objective: We confirmed the effectiveness of photometric method for the determination of histamine in fish and fish products by obtaining and analyzing validation characteristics. Results: Based on the results of validation, it has been substantiated and experimentally proved that the photometric method of quantitative determination of histamine can be correctly reproduced and is suitable for the quality control of measurements. The most acceptable results were obtained for histamine concentrations in the range from 50.0 ± 0.1 to 200.0 ± 0.5 mg/kg. The lower histamine concentrations are characterized by a higher percentage of total uncertainty, although it is also within acceptable values. Conclusions: Evaluation of the obtained validation characteristics indicates that this technique can be used to quantify the content of histamine in fish and fish products and is not inferior in accuracy and correctness to the method of high performance liquid chromatography.

scholarly journals DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYTICAL METHOD FOR DETERMINATION OF AMOXICILLIN IN CAPSULES

2016 ◽  
Vol 13 (26) ◽  
pp. 78-87
Author(s):  
Tiago Hickman IGLIN ◽  
Flávia Nathiely Silveira FACHEL ◽  
Amanda Gonçalves GUWZINSKI ◽  
Airton Monza da SILVEIRA ◽  
Filipe de Medeiros ALBANO ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Forced Degradation ◽  
Semisynthetic Penicillin ◽  
The Stability ◽  
Development And Validation

Amoxicillin, substance-related to semisynthetic penicillin, has been widely used to treat infections caused by various microorganisms, however reports of suitable methods for the quantitative determination and indicative of the stability of formulations containing this substance are rare. Due to lack of studies on the forced degradation of the substance and on the need to monitor the quality of this type of formulation was proposed and validated a method for the determination of amoxicillin content in capsules by high-performance liquid chromatography - HPLC for the quality control of amoxicillin capsules, allowing the provision of useful information about the characteristics of this type of formulation and its stability. The method was validated for parameters of linearity, specificity, accuracy, precision, and robustness

scholarly journals Simultaneous Quantification of Two Flavonoids in Morus alba by High Performance Liquid Chromatography Coupled with Photodiode Array Detector

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301
Author(s):  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Morus Alba ◽  
Array Detector ◽  
Root Bark ◽  
Relative Standard ◽  
Photodiode Array

The root bark of Morus alba L. (Family: Moraceae) is an important medicinal herb in many countries and has long been used as a traditional medicine for the treatment of cough, fever, blood pressure reduction, and respiratory diseases. In the present study, the simultaneous determination of two flavonoids, kuwanon G and morusin, for quality control of M alba was conducted using high-performance liquid chromatography (HPLC) equipped with photodiode array (PDA) detector. The column used for separation of kuwanon G and morusin was a Gemini C18 analytical column maintained at 45°C. The mobile phase for efficient separation of two analytes was flowed 0.1% (v/v) aqueous formic acid-acetonitrile with gradient elution. The detection wavelength for quantification was set at 266 nm. The optimized method showed good linearity with coefficients of determination of 0.9998 within the tested concentration ranges. The limits of detection for the two flavonoids, kuwanon G and morusin, were 0.69 μg/mL and 0.35 μg/mL and the limits of quantification of kuwanon G and morusin, were 2.10 μ/mL and 1.07 μg/mL. The recoveries were 98.40–111.55% and the relative standard deviation (RSD) value was within 3.50%. The RSD values of intra- a g d interday precisions were 0.08–0.70% and 0.06-0.48%, respectively. The amounts of kuwanon G and morusin were 1.94-2.26 mg/g and 1.05–1.12 mg/g. The established HPLC-PDA method will help to improve the quality control of M. alba and related products.

Establishment and Evaluation of Determination of Cinnamaldehyde in Baoyuanqingxue Granules by HPLC

2014 ◽  
Vol 998-999 ◽  
pp. 372-377 ◽  
Author(s):  
Qiang Wu ◽  
Chang Hong Wang ◽  
Pu Wang ◽  
Xiang Rong Liu
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Extraction Method ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Column Temperature ◽  
Average Recovery

To examine the extraction method and chromatographic conditions that affect the determination of cinnamaldehyde in Baoyuanqingxue granules and make clinical evaluation about the determination of cinnamaldehyde.Ultrasonic methanol extraction was used before the detemination of cinnamaldehyde in Baoyuanqingxue granules. High Performance Liquid chromatography (HPLC) method was applied to detect samples. The SB-C18 column (Agilent, ZORBAX, 4.6×150mm, 5μm) was adopted, the mobile phase was acetonitrile-water (35:65) at the flow rate of 1.00mL•min-1 with DAD detection wavelength at 290nm, the volume of injection was 20μL and the column temperature was 30°C. The resolution between cinnamaldehyde and other peaks was good. The calibration curve was linear in the range of 0.5035~50.35μg•mL-1(r=0.99976). The average recovery (n=6) of cinnamaldehyde was 99.2% with RSD of 0.5%. The HPLC-DAD method to detect the content of cinnamaldehyde in Baoyuanqingxue granules is simple and accurate. It can be used for quality control of cinnamaldehyde in Baoyuanqingxue granules.

Matrine Determination in Compound Sophora Alopecuroides Suppositories by High-Performance Liquid Chromatography

2013 ◽  
Vol 634-638 ◽  
pp. 1001-1004
Author(s):  
Hui Xiang Hu ◽  
Xuan Zhang ◽  
Zhimin Zhang
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Average Recovery ◽  
Sophora Alopecuroides ◽  
Rp Hplc

The determination of matrine in compound sophora alopecuroides suppositories was carried out by reversed-phase high-performance liquid chromatography(RP-HPLC). Elite ODS C18(250mm×4.5mm, 5µm) column was used and acetonitrile -anhydrous ethanol-water-triethy lamine (15:22.5:62.5:0.01) was selected as the mobile phase. Meanwhile the detection wavelength 215nm was used. The linearity of matrine was in the range of 0.74-5.18µg，r=0.9991，and the average recovery was 98.52%，RSD was 1.54%. The method was proved to be simple, accurate, sensitive and reliable. It can be used for the quality control of compound sophora alopecuroides suppositories.

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