scholarly journals In Vivo Study of Tricomponent Resorbable Calcium Phosphate Bone Cement Based on Tricalcium Phosphate

2014 ◽  
pp. 72-77
Author(s):  
National Research Mamonov ◽  
National Research Chemis ◽  
National Research Drize ◽  
National Research Proskurina ◽  
I. I. Kryazhkov ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Tricalcium Phosphate ◽  
Bone Defects ◽  
Morphologic Study ◽  
Hemostatic Effect ◽  
Calcium Phosphate Bone Cement ◽  
Intertrabecular Space ◽  
Peripheral Bone

Results of experimental morphologic study of tricomponent resorbable calcium phosphate bone cement (CPhC), based on tricalcium phosphate for the filling of defect as a temporary bearing resorbable matrix are presented. Study was performed on soviet chinchilla rabbits weighting 3200-3500 g. The model of critical spongy bone defect was used. At different observation terms (6, 9 and 12 months) gradual substitution of biomaterial with newly formed bone tissue from periphery to the center was observed with complete cement resorption 12 months after surgery. By mechanic characteristics newly formed bone in the defect was stronger than the surrounding trabecular one. It was stated that material possessed hemostatic effect and moderate toxicity. Peripheral bone marrow maintained its cellularity at all terms, gradually filling intertrabecular space of newly formed bone. Achieved data enable to recommend wide used of CPhC for bone defects substitution.

scholarly journals In Vivo Study of Tricomponent Resorbable Calcium Phosphate Bone Cement Based on Tricalcium Phosphate

2014 ◽  
Vol 21 (1) ◽  
pp. 72-77
Author(s):  
National Research Center for Hematology, Moscow, RF Mamonov ◽  
National Research Center for Hematology, Moscow, RF Chemis ◽  
National Research Center for Hematology, Moscow, RF Drize ◽  
National Research Center for Hematology, Moscow, RF Proskurina ◽  
I. I Kryazhkov ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Tricalcium Phosphate ◽  
Bone Defects ◽  
Morphologic Study ◽  
Hemostatic Effect ◽  
Calcium Phosphate Bone Cement ◽  
Intertrabecular Space ◽  
Peripheral Bone

Results of experimental morphologic study of tricomponent resorbable calcium phosphate bone cement (CPhC), based on tricalcium phosphate for the filling of defect as a temporary bearing resorbable matrix are presented. Study was performed on soviet chinchilla rabbits weighting 3200-3500 g. The model of critical spongy bone defect was used. At different observation terms (6, 9 and 12 months) gradual substitution of biomaterial with newly formed bone tissue from periphery to the center was observed with complete cement resorption 12 months after surgery. By mechanic characteristics newly formed bone in the defect was stronger than the surrounding trabecular one. It was stated that material possessed hemostatic effect and moderate toxicity. Peripheral bone marrow maintained its cellularity at all terms, gradually filling intertrabecular space of newly formed bone. Achieved data enable to recommend wide used of CPhC for bone defects substitution.

scholarly journals Preliminary in Vivo Evaluation of Bone Healing in Critical Size Bone Defects Implanted with an Injectable Calcium Phosphate Bone Cement (Osteopaste)

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Zunariah Buyong ◽  
Mohd Shukrimi Awang ◽  
Ahmad Razali Md Ralib @ Md Raghib
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Healing Process ◽  
Bone Defects ◽  
Calcium Phosphate Bone Cement ◽  
Rabbit Tibia

Introduction: A novel injectable calcium phosphate bone cement (osteopaste) has been developed. Its potential application in orthopaedics as a filler of bone defects has been studied. The biomaterial was composed of tetra-calcium phosphate (TTCP) and tricalcium phosphate (TCP) powder. The aim of the present study was to evaluate the healing process of osteopaste in rabbit tibia. Materials and method: The implantation procedure was carried out on thirty-nine of New Zealand white rabbits. The in vivo bone formation was investigated by either implanting the Osteopaste, Jectos or MIIG – X3 into a critical size defect (CSD) model in the proximal tibial metaphysis. CSD without treatment served as negative control. After 1 day, 6 and 12 weeks, the rabbits were euthanized, the bone were harvested and subjected for analysis. Results: Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. There was direct contact between osteopaste material and host bone. The new bone was seen bridging the defect. Conclusion: The result showed that Osteopaste could be a new promising biomaterial for bone repair and has a potential in bone tissue engineering.

Development of injectable chitosan/biphasic calcium phosphate bone cement and in vitro and in vivo evaluation

2020 ◽  
Vol 15 (5) ◽  
pp. 055038
Author(s):  
Sirirat T. Rattanachan ◽  
Nuan La-ong Srakaew ◽  
Paritat Thaitalay ◽  
Oranich Thongsri ◽  
Rawee Dangviriyakul ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Biphasic Calcium Phosphate ◽  
In Vivo Evaluation ◽  
Calcium Phosphate Bone Cement

Improving osteogenesis of calcium phosphate bone cement by incorporating with manganese doped β-tricalcium phosphate

2020 ◽  
Vol 109 ◽  
pp. 110481 ◽  
Author(s):  
Tingting Wu ◽  
Haishan Shi ◽  
Yongyi Liang ◽  
Teliang Lu ◽  
Zefeng Lin ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Tricalcium Phosphate ◽  
Calcium Phosphate Bone Cement

In vitro and in vivo effectiveness of a novel injectable calcitonin-loaded collagen/ceramic bone substitute

2021 ◽  
pp. 088532822198998
Author(s):  
Karl Wu ◽  
Yu-Chun Chen ◽  
Shang M Lin ◽  
Chih-Hung Chang
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Degradation Rate ◽  
Rabbit Model ◽  
Type I ◽  
Calcium Phosphate Bone Cement ◽  
Osteogenic Effect ◽  
Composite Bone

This study aimed to evaluate the effectiveness of a novel calcitonin-loaded calcium phosphate composite bone cement in vitro and in vivo. The novel composite bone cements were composed of NuROs injectable bone graft substitute, type I collagen, and/or salmon calcitonin. The setting time, porosity, wettability, compressive strength, compressive modulus, and crystallographic structures of cement specimens were determined. Degradation rate, calcitonin release rate, and osteoinductivity were assessed in vitro. In addition, osteogenic effect was examined in a rabbit model of femoral defect. The results revealed that addition of collagen/calcitonin did not substantially alter physical properties and degradation rate of bone cement specimens. Calcitonin was released into culture medium in a two-phase manner. Osteogenic effect of conditioned medium derived from calcitonin containing bone cement was observed. Finally, de novo bone growth and bone mineralization across the bone defect area were observed in rabbits after implantation of composite bone cement specimens. In conclusion, this novel calcitonin-loaded composite calcium phosphate bone cement exhibits biocompatibility, bioresorbability, osteoinductivity, and osteoconductivity, which may be suitable for clinical use.

scholarly journals Early Results of the Study of Reparative Peculiarities of Various Osteoplastic Materials in Experimental Bone Defects

2017 ◽  
pp. 40-47
Author(s):  
K. A. Egiazaryan ◽  
G. D. Lazishvili ◽  
K. I. Akmataliev ◽  
A. P. Ettinger ◽  
A. P. Rat’Ev ◽  
...  
Keyword(s):  
Bone Cement ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Calcium Sulphate ◽  
Bone Defects ◽  
Spongy Bone ◽  
Early Results ◽  
Calcium Phosphate Bone Cement ◽  
Bone Trabeculae ◽  
Osteoplastic Material

Purpose. To determine the optimum osteoplastic material for activation of reparative osteogenesis and substitution of traumatic defects in metaepiphyseal spongy bone tissue.Material and methods.Comparative experimental morphological study was performed on 12 matured male Chinchilla rabbits with body weight 2500-2800g. A model of critical defects of spongy bone tissue was used. Bone defects were filled with 3 types of osteoplastic material: composite calcium sulphate bone cement; xenogenous hydroxyapatite-based material with granulated paste of synthetic peptides (P-15); β-tricalcium phosphate-based material with gauging liquid (calcium phosphate bone cement).Results.Implantation of calcium sulphate bone cement showed rapid osteogenesis stimulation at terms 1.5 months and reduction of newly formed bone tissue mass by 3rd month due to active resorption of the residual material. Implantation of hydroxyapatite-based material with P-15 resulted in osteogenesis stimulation on its surface however because of its mechanical instability and absence of active resorption of that material only a moderate formation of bone trabeculae was observed. At implantation of β-tricalcium phosphate-based material an active resorption of osteoplastic material, formation of trabecular system and its reorganization into trabecular network of femoral metaepiphyseal spongy bone accompanied by the formation of mature bone trabeculae was noted by month 3.Conclusion. Mechanical stability of osteoplastic material and subsequent gradual resorption as well as formation of mature bine trabeculae indicates the efficacy of β-tricalcium phosphate-based material.

scholarly journals In Vitro Evaluation of Calcium Phosphate Bone Cement Composite Hydrogel Beads of Cross-Linked Gelatin-Alginate with Gentamicin-Impregnated Porous Scaffold

2021 ◽  
Vol 14 (10) ◽  
pp. 1000
Author(s):  
Shih-Ming Liu ◽  
Wen-Cheng Chen ◽  
Chia-Ling Ko ◽  
Hsu-Ting Chang ◽  
Ya-Shun Chen ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Antibacterial Activity ◽  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Defects ◽  
Composite Hydrogel ◽  
Hydrogel Beads ◽  
Hydrogel Composite ◽  
Calcium Phosphate Bone Cement ◽  
Hydrogel Composites

Calcium phosphate bone cement (CPC) is in the form of a paste, and its special advantage is that it can repair small and complex bone defects. In the case of open wounds, tissue debridement is necessary before tissue repair and the subsequent control of wound infection; therefore, CPC composite hydrogel beads containing antibiotics provide an excellent option to fill bone defects and deliver antibiotics locally for a long period. In this study, CPC was composited with the millimeter-sized spherical beads of cross-linked gelatin–alginate hydrogels at the different ratios of 0 (control), 12.5, 25, and 50 vol.%. The hydrogel was impregnated with gentamicin and characterized before compositing with CPC. The physicochemical properties, gentamicin release, antibacterial activity, biocompatibility, and mineralization of the CPC/hydrogel composites were characterized. The compressive strength of the CPC/hydrogel composites gradually decreased as the hydrogel content increased, and the compressive strength of composites containing gentamicin had the largest decrease. The working time and setting time of each group can be adjusted to 8 and 16 min, respectively, using a hardening solution to make the composite suitable for clinical use. The release of gentamicin before the hydrogel beads was composited with CPC varied greatly with immersion time. However, a stable controlled release effect was obtained in the CPC/gentamicin-impregnated hydrogel composite. The 50 vol.% hydrogel/CPC composite had the best antibacterial effect and no cytotoxicity but had reduced cell mineralization. Therefore, the optimal hydrogel beads content can be 25 vol.% to obtain a CPC/gentamicin-impregnated hydrogel composite with adequate strength, antibacterial activity, and bio-reactivity. This CPC/hydrogel containing gentamicin is expected to be used in clinical surgery in the future to accelerate bone regeneration and prevent prosthesis infection after surgery.

scholarly journals Early Results of the Study of Reparative Peculiarities of Various Osteoplastic Materials in Experimental Bone Defects

2017 ◽  
Vol 24 (2) ◽  
pp. 40-47
Author(s):  
K. A Egiazaryan ◽  
G. D Lazishvili ◽  
K. I Akmataliev ◽  
A. P Ettinger ◽  
A. P Rat’ev ◽  
...  
Keyword(s):  
Bone Cement ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Calcium Sulphate ◽  
Bone Defects ◽  
Spongy Bone ◽  
Early Results ◽  
Calcium Phosphate Bone Cement ◽  
Bone Trabeculae ◽  
Osteoplastic Material

Purpose. To determine the optimum osteoplastic material for activation of reparative osteogenesis and substitution of traumatic defects in metaepiphyseal spongy bone tissue. Material and methods. Comparative experimental morphological study was performed on 12 matured male Chinchilla rabbits with body weight 2500-2800g. A model of critical defects of spongy bone tissue was used. Bone defects were filled with 3 types of osteoplastic material: composite calcium sulphate bone cement; xenogenous hydroxyapatite-based material with granulated paste of synthetic peptides (P-15); β-tricalcium phosphate-based material with gauging liquid (calcium phosphate bone cement). Results. Implantation of calcium sulphate bone cement showed rapid osteogenesis stimulation at terms 1.5 months and reduction of newly formed bone tissue mass by 3rd month due to active resorption of the residual material. Implantation of hydroxyapatite-based material with P-15 resulted in osteogenesis stimulation on its surface however because of its mechanical instability and absence of active resorption of that material only a moderate formation of bone trabeculae was observed. At implantation of β-tricalcium phosphate-based material an active resorption of osteoplastic material, formation of trabecular system and its reorganization into trabecular network of femoral metaepiphyseal spongy bone accompanied by the formation of mature bone trabeculae was noted by month 3. Conclusion. Mechanical stability of osteoplastic material and subsequent gradual resorption as well as formation of mature bine trabeculae indicates the efficacy of β-tricalcium phosphate-based material.

scholarly journals The Development of a Magnesium-Releasing and Long-Term Mechanically Stable Calcium Phosphate Bone Cement Possessing Osteogenic and Immunomodulation Effects for Promoting Bone Fracture Regeneration

2022 ◽  
Vol 9 ◽  
Author(s):  
Jun Wu ◽  
Feihong Liu ◽  
Zejin Wang ◽  
Yuan Liu ◽  
Xiaoli Zhao ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Mechanical Stability ◽  
Bone Grafts ◽  
Chemical Components ◽  
Allogeneic Bone ◽  
Bone Maturation ◽  
Calcium Phosphate Bone Cement

Bone grafts are commonly used for the treatment of critical sized bone defects. Since the supply of autologous bone is insufficient, allogeneic bone grafts have been used most of the time. However, the poor osteogenic property of allogeneic bone grafts after pretreatment results in delayed union, non-union, or even occasional deformity. Calcium phosphate cement (CPC) is one of the most promising bone filling materials due to its good biocompatibility and similar chemical components as natural bone. However, clinical applications of CPC were hampered by limited osteogenic effects, undesired immune response which results in resorption, and poor mechanical stability in vivo. Magnesium (Mg) has been proven to trigger bone regeneration through modulating cell behaviors of mesenchymal stem cells and macrophages significantly. Unfortunately, the degradation raters of pure Mg and Mg oxide are extremely fast, resulting in early collapse of Mg contained CPC. In this study, we developed a novel magnesium contained calcium phosphate bone cement (Mg-CPC), possessing long-term mechanical stability and osteogenic effects through sustained release of Mg. Furthermore, in vitro studies showed that Mg-CPC had no cytotoxic effects on hBMMSCs and macrophage RAW 264.7, and could enhance the osteogenic differentiation as determined by alkaline phosphate (ALP) activity and calcium nodule staining, as well as suppress the inflammatory as determined by expression of anti-inflammatory cytokine IL-1RA. We also found that Mg-CPC promoted new bone formation and bone maturation in vivo. These results suggest that Mg-CPC should be a good substitute material for bone grafts in clinical use.

Sign in / Sign up

Export Citation Format

Share Document