scholarly journals Hairy induction from Impatiens balsamina L. using fourteen Agrobacterium rhizogenes strains

2016 ◽  
Vol 19 (2) ◽  
pp. 38-47
Author(s):  
Hai Trung Phan ◽  
Phuong Ngo Diem Quach ◽  
Nhut Nhu Nguyen
Keyword(s):  
Hairy Root ◽  
Immersion Time ◽  
Root Formation ◽  
Biological Activities ◽  
Root Production ◽  
Root Cultures ◽  
Impatiens Balsamina ◽  
Strain Type ◽  
Basal Media ◽  
Agrobacterium Rhizogenes Strains

Impatiens balsamina L. is a plant commonly grown in Vietnam. It has long been used as traditional medicine. All of the secondary metabolites produced by Impatiens balsamina L. root possessed biological activities. The main advantage of using hairy root cultures is their ability of growing fast in defined basal media without supplementation of phytohormones. The aim of this research is to invest some factors that could affect on the hairy root production such as A. rhizogenes strain, type of tissue, OD concentration, immersion time and co-cultivation time. The results showed that 3 strain A. rhizogenes C02, C18 and C26 isolated from nature in Vietnam could induce the best hairy root formation at the 0.5–1.0 OD concentration bacteria with 5’ of immersion time and 72 hours of co-cultivating. Among them, C02 is the strain that could offer the best result in B5 medium

scholarly journals Production of Prenylated Stilbenoids in Hairy Root Cultures of Peanut (Arachis hypogaea) and Its Wild Relatives A. ipaensis and A. duranensis via an Optimized Elicitation Procedure

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 509 ◽  
Author(s):  
Lingling Fang ◽  
Tianhong Yang ◽  
Fabricio Medina-Bolivar
Keyword(s):  
Arachis Hypogaea ◽  
Hairy Root ◽  
Biological Activities ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Orthogonal Array Design ◽  
Biotic And Abiotic Stresses ◽  
Elicitor Treatment ◽  
Elicitation Procedure ◽  
Medium Supplementation

Prenylated stilbenoids are phenolic compounds produced in a small number of plants such as peanut (Arachis hypogaea) to counteract biotic and abiotic stresses. In addition to their role in plant defense, they exhibit biological activities with potential application in human health. Whereas non-prenylated stilbenoids such as resveratrol are commercially available, the availability of prenylated stilbenoids is limited. To this end, hairy root cultures of peanut were developed as an elicitor-controlled bioproduction platform for prenylated stilbenoids. An orthogonal array design approach led to the elucidation of an optimized elicitation procedure consisting of co-treatment of the hairy root cultures with 18 g/L methyl-β-cyclodextrin, 125 µM methyl jasmonate, 3 mM hydrogen peroxide (H2O2) and medium supplementation with additional 1 mM magnesium chloride. After 168-h of elicitor treatment, the combined yield of the prenylated stilbenoids arachidin-1, arachidin-2, arachidin-3 and arachidin-5 reached approximately 750 mg/L (equivalent to 107 mg/g DW). Moreover, hairy root cultures from the wild Arachis species A. duranensis and A. ipaensis were developed and shown to produce prenylated stilbenoids upon elicitor treatment. These wild Arachis hairy root lines may provide a platform to elucidate the biosynthetic origin of prenylated stilbenoids in peanut.

Establishment of Momordica charantia hairy root cultures for the production of phenolic compounds and determination of their biological activities

2014 ◽  
Vol 118 (3) ◽  
pp. 545-557 ◽  
Author(s):  
Muthu Thiruvengadam ◽  
Nagella Praveen ◽  
K. M. Maria John ◽  
Ye-Sul Yang ◽  
Seung-Hyun Kim ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Hairy Root ◽  
Biological Activities ◽  
Momordica Charantia ◽  
Hairy Root Cultures ◽  
Root Cultures

Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

2006 ◽  
Vol 61 (5-6) ◽  
pp. 351-356 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Aleksandra Królicka ◽  
Halina Wysokińska
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Rosmarinic Acid ◽  
Root Culture ◽  
Dry Weight ◽  
Salvia Officinalis ◽  
Root Cultures ◽  
Hairy Root Line ◽  
Salvia Officinalis L ◽  
Agrobacterium Rhizogenes Strains

Shoots of Salvia officinalis, a medicinally important plant, were infected with Agrobacterium rhizogenes strains ATCC 15834 and A4 which led to the induction of hairy roots in 57% and 37% of the explants, respectively. Seven lines of hairy roots were established in WP liquid medium under light and dark conditions. The transformed nature of the root lines was confirmed by polymerase chain reaction using rolB and rolC specific primers. Transformed root cultures of Salvia officinalis showed variations in biomass and rosmarinic acid production depending on the bacterial strain used for transformation and the root line analyzed. Both parameters (growth and rosmarinic acid content) of ATCC 15834-induced lines were significantly higher than the A4-induced lines. The maximum accumulation of rosmarinic acid (about 45 mg g-1 of dry weight) was achieved by hairy root line 1 (HR-1) at the end of the culture period (45D50 days). The level was significantly higher than that found in untransformed root culture (19 mg g-1 of dry wt).

scholarly journals Induction of Platycodon grandiflorum hairy roots through the mediation of four Agrobacterium rhizogenes strains

2016 ◽  
Vol 19 (4) ◽  
pp. 64-75
Author(s):  
Phuong Dong Tra ◽  
Phuong Thi Bach Vu ◽  
Phuong Ngo Diem Quach
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Biological Activities ◽  
Root Induction ◽  
Infection Frequency ◽  
Platycodon Grandiflorum ◽  
Metabolites Production ◽  
Valuable Compounds ◽  
Agrobacterium Rhizogenes Strains

Balloon flower (Platycodon grandiflorum (Jacq.) A. DC.), the only species in Platycodon genus (Campanulaceae), is mainly distributed in East Asia. The rhizomes of P. grandiflorum, a traditional herbal medicine, have been widely used for the treatment of cough, sore throat, asthma, tuberculosis and other diseases. Recently, pharmacological researches identified important biological activities compounds in the rhizomes. Thus, to study and extract valuable compounds, a hairy root induced technique was achieved on P. grandiflorum for stable material with fast growth rates (in hormone-free media) and metabolites production. To achieve this, the “natural genetic tool” Agrobacterium rhizogenes, which can transfer DNA segments into genome of plant, was exploited. The results suggested two (A. rhizogenes ATCC 15834 and C34) of four A. rhizogenes strains could induce hairy roots. RolB and rolC genes, which are responsible for the induction of hairy roots, were inserted into the genome of hairy roots. Leaves had the highest infection frequency of hairy root induction 100 %. The optimization of protocol, including time of immersion and co-culture, had the best results with 10 and 15 mins (10 mins for A. rhizogenes ATCC 15834 and 15 mins for A. rhizogenes C34) and 72 hours, respectively. In the future, this protocol, which was described in this paper, should be useful for studying and isolating valuable compounds from P. grandiflorum hairy root cultures.

Production of anthraquinones, phenolic compounds and biological activities from hairy root cultures of Polygonum multiflorum Thunb.

PROTOPLASMA ◽  
2013 ◽  
Vol 251 (3) ◽  
pp. 555-566 ◽  
Author(s):  
Muthu Thiruvengadam ◽  
Nagella Praveen ◽  
Eun-Hye Kim ◽  
Seung-Hyun Kim ◽  
Ill-Min Chung
Keyword(s):  
Phenolic Compounds ◽  
Hairy Root ◽  
Biological Activities ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Polygonum Multiflorum

scholarly journals Biological activities and hairy roots induction of Abutilon indicum (L.)

2016 ◽  
Vol 19 (4) ◽  
pp. 95-104
Author(s):  
Phuong Thi Bach Vu ◽  
Minh Thi Thanh Hoang ◽  
Hong Thi Anh Pham ◽  
Phuong Ngo Diem Quach
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Biological Activities ◽  
Reducing Power ◽  
Artemia Salina ◽  
Root Production ◽  
Pcr Analysis ◽  
Root Extract ◽  
Root Induction ◽  
Abutilon Indicum

Abutilon indicum L. belonging to Malvaceae is used as traditional medicinal herbs to treat syphilis, hypoglycemia, hepatic disorders, and malaria... Because of the medicinal value of A. indicum, the aims of this study are the evaluation of bioactivities of A. indicum and production of transformed hairy root for pharmaceutical production. In this study, the reducing power of ethanol root extract and stem extract are higher than that of leave. The root extract exhibited potent brine shrimp (Artemia salina) lethality with LC50 37.04 μg/mL. The α-glucosidase and acetylcholinesterase inhibitory activities of the root extract are the highest. These results showed that roots are higher bioactivities than stems and leaves. This study induced successfully hairy roots of A. indicum L. via Agrobacterium rhizogenes ATCC 15834 in the plant cells. The frequency of hairy root and number of hairy root induction from the wounded sites of leaves are the highest (88.66 % and 8.66 roots). The stable introduction of the rolB and rolC genes of A. rhizogenes strain 15834 into A. indicum plants was confirmed by PCR analysis. Besides, the absence of the virD gene confirmed hairy roots as a bacteria-free. Subsequently, these results demonstrated that A. indicum, particularly the roots, have great potential as pharmacological values and hairy root production maybe used for pharmaceutical sources.

Elicitor-enhanced Silymarin production in hairy root cultures of Silybum marianum L. Gaertn

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Hasanloo ◽  
H Rahnama ◽  
R Sepehrifar ◽  
MR Shams
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Silybum Marianum

Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Clone Selection ◽  
Selection Of
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